Gamma‐Ray‐Irradiated Red Blood Cells Stored in Mannitol‐Adenine‐Phosphate Medium: Rheological Evaluation and Susceptibility to Oxidative Stress

Cicha, Iwona; Suzuki, Yoshio; Tateishi, Norihiko; Shiba, Masayuki; Muraoka, Masako; Tadokoro, Kenji; Maeda, Nobuji

doi:10.1046/j.1423-0410.2000.7920075.x

Cited by 42 publications

(25 citation statements)

References 31 publications

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“…1A-C, the increase in the external potassium concentration of RBC preparations was markedly enhanced by the irradiation, which is consistent with a previous report. 16) DPM, which is widely used in the treatment of cardiovascular disease, binds noncovalently with high affinity (K d ϭ 1.2 mM) to band 3, which is the most abundant protein on the RBC membrane with about a million copies per cell. 14) It has been reported that dipyridamole exerts an inhibitory effect on lipid peroxidation 17) and acts as a scavenger of superoxide anion, hydroxyl radical and peroxyl radical.…”

Section: Resultsmentioning

confidence: 99%

Leakage of Potassium from Red Blood Cells following Gamma Ray Irradiation in the Presence of Dipyridamole, Trolox, Human Plasma or Mannitol

Hirayama

Abe

Azuma

et al. 2005

Biological & Pharmaceutical Bulletin

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Transfusion-associated graft-versus-host disease (TA-GVHD) is a complication of blood transfusion that results from the engraftment and clonal expansion of allogenic donor white cells.1) Although TA-GVHD is rare, patients respond poorly to treatment and the disorder is usually fatal. 2)Radiation treatment of blood components is currently used to prevent TA-GVHD. 3) This procedure is based on the large difference between the radiation sensitivities of T-lymphocytes and red blood cells (RBCs).3,4) Thus, one has to apply a dose high enough to destroy almost all the T-lymphocytes while causing as little damage as possible to RBCs. Recent studies indicate that the minimal dose required to fulfill this requirement is from 15 to 50 Gy. 5,6) Doses of this order of magnitude cause only minor changes in the essential constituents of RBCs, e.g. intracellular hemoglobin, ATP, membrane lipid and proteins. However, they promote the leakage of potassium from RBCs. 7,8) There have been concerns about possible side effects, such as hyperpotassemia and cardiac arrest, of the transfusion of blood components with an elevated potassium concentration. [9][10][11] The irradiation of aqueous solutions can lead to the generation of reactive oxygen species (ROS) such as the hydroxyl radical which is a powerful oxidant and can interact with lipids and proteins in the membranes of RBCs.12) It was reported that the increase in the extracellular potassium concentrations of RBC preparation on photosensitization resulted from the oxidative damage of RBC membranes by ROS produced by photosensitization, and furthermore, that the increase was sufficiently inhibited by the addition of an antioxidant such as dipyridamole (DPM) or Trolox before the photosensitization.13,14) Therefore, the leakage of potassium caused by the irradiation may also be due to the damage of RBC membranes by ROS. Although the use of an antioxidant could potentially minimize the leakage which may result from the irradiation, trials using antioxidants to prevent potassium leakage are rare. 15) Here we investigated the effect of antioxidants DPM, Trolox, human plasma or mannitol on the leakage of potassium ascribed to the irradiation of RBCs. MATERIALS AND METHODSPreparation of RBC Samples and Irradiation RBC in MAP solution (RC-MAP) were prepared according to the standard protocol of the Hokkaido Red Cross Blood Center. Briefly, after the centrifugation of donated human blood (400 ml) at 3000 g for 8 min, the supernatant was removed and 92 ml of MAP solution (1.34 g D-mannitol, 0.013 g adenin, 0.086 g sodium dihydrogenphosphate, 0.138 g sodium citrate dihydrate, 0.018g citric acid, 0.663 g D-glucose, and 0.457 g sodium chloride) was added (hematocrit: approximately 60%). RC-MAP stored for 7 d at 4°C was mixed with the same volume of MAP solution containing DPM, Trolox, human plasma or mannitol. RBC preparations (30 ml) in plastic tubes (50 ml centrifuge tube; IWAKI Inc., Tokyo, Japan) were agitated for 2 h at room temperature and subsequently exposed to 30 Gy of gamma ray i...

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Section: Resultsmentioning

confidence: 99%

Leakage of Potassium from Red Blood Cells following Gamma Ray Irradiation in the Presence of Dipyridamole, Trolox, Human Plasma or Mannitol

Hirayama

Abe

Azuma

et al. 2005

Biological & Pharmaceutical Bulletin

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“…As shown previously, the mechanical behavior of RBC is predominantly determined by cell shape, surface area-to-volume ratio, membrane and skeleton condition, and surface charge [1,2,3], properties that are altered during cold storage of RBC [4,5]. Cold storage of RBC in blood banks has been reported to be associated with changes in various RBC properties, including increased cell volume [6], oxidation of membrane protein and lipids [7], loss of cellular antioxidant capability [8], changes in K + and Na + concentration [9], changes in lipid in/out distribution on the RBC surface [8,10], and decreased cell membrane area [11,12]. Storage-induced damage to RBC, which can be noted as early as the second week of storage and progresses with storage duration [4], has been shown to impair the functionality of stored RBC (St-RBC) [5] and to facilitate their removal from circulation following transfusion [13,14,15,16].…”

Section: Introductionmentioning

confidence: 99%

Storage-Induced Damage to Red Blood Cell Mechanical Properties Can Be Only Partially Reversed by Rejuvenation

Barshtein

Gural²,

Manny³

et al. 2014

Transfus Med Hemother

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Background: The storage of red blood cells (RBC) is associated with impairment of their properties that can induce a circulatory risk to recipients. In a preceding study (2009), we reported that post-storage rejuvenation (RJ) of stored RBC (St-RBC) efficiently reduced the storage-induced RBC/endothelial cell interaction, while only partially reversing the level of intracellular Ca²⁺, reactive oxygen species, and surface phosphatidylserine. In the present study, we examined the RJ effectiveness in repairing St-RBC mechanical properties. Methods: RBC, stored in CPDA-1 without pre-storage leukoreduction, were subjected to post-storage RJ, and the deformability, osmotic fragility (OF), and mechanical fragility (MF) of the rejuvenated St-RBC (St-RBC_Rj) were compared to those of untreated St-RBC and of freshly-collected RBC (F-RBC). Results: 5-week storage considerably increased OF and MF, and reduced the deformability of St-RBC. All alterations were only partially (40-70%) reversed by RJ, depending on the extent of the damage: the greater the damage, the lesser the relative effect of RJ. Conclusion: The findings of the present and preceding studies suggest that different St-RBC properties are differentially reversed by RJ, implying that some of the changes occur during storage and are irreversible.

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“…Erythrocyte deformability has become a focus of interest for surgeons [70,76] and transplantologists [17,49]. In transfusion medicine, assessment of RBC deformability [19,33] is a part of quality control.…”

Section: Introductionmentioning

confidence: 99%

Red blood cell-deformability measurement: Review of techniques

Musielak¹

2009

Clinical Hemorheology and Microcirculation

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Cell-deformability characterization involves general measurement of highly complex relationships between cell biology and physical forces to which the cell is subjected. The review takes account of the modern technical solutions simulating the action of the force applied to the red blood cell in macro-and microcirculation. Diffraction ektacytometers and rheoscopes measure the mean deformability value for the total red blood cell population investigated and the deformation distribution index of individual cells, respectively. Deformation assays of a whole single cell are possible by means of optical tweezers. The single cell-measuring setups for micropipette aspiration and atomic force microscopy allow conducting a selective investigation of deformation parameters (e.g., cytoplasm viscosity, viscoelastic membrane properties). The distinction between instrument sensitivity to various RBC-rheological features as well as the influence of temperature on measurement are discussed. The reports quoted confront fascinating possibilities of the techniques with their medical applications since the RBC-deformability has the key position in the etiology of a wide range of conditions.

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Gamma‐Ray‐Irradiated Red Blood Cells Stored in Mannitol‐Adenine‐Phosphate Medium: Rheological Evaluation and Susceptibility to Oxidative Stress

Abstract: The decreased deformability of gamma-ray-irradiated RBCs in MAP medium was mainly induced by dehydration due to potassium loss, and the membrane lipids and proteins were stably preserved against oxidative stress.

Cited by 42 publications

References 31 publications

Leakage of Potassium from Red Blood Cells following Gamma Ray Irradiation in the Presence of Dipyridamole, Trolox, Human Plasma or Mannitol

Leakage of Potassium from Red Blood Cells following Gamma Ray Irradiation in the Presence of Dipyridamole, Trolox, Human Plasma or Mannitol

Storage-Induced Damage to Red Blood Cell Mechanical Properties Can Be Only Partially Reversed by Rejuvenation

Red blood cell-deformability measurement: Review of techniques

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