Gamma ray mutagenesis in a strain of Escherichia coli deficient in DNA polymerase I

Bridges, B.A.; Mottershead, R.P.

doi:10.1038/hdy.1972.84

Cited by 17 publications

(3 citation statements)

References 20 publications

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“…Therefore, the 2-induction potency and radiosensitivity are both higher than that for wild-type cells and parallel the LET dependence of `normal' ssb induction . As mentioned previously, the production of these ssb decreases slowly with increasing LET ; a plateau was observed at LET values lower than 100 keV Jµm (Bridges and Mottershead 1972) .…”

Section: the Role Of Genotype In 2-prophage Inductionmentioning

confidence: 64%

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λ-prophage Induction in Repair-deficient and Wild TypeE. ColiStrains by γ-rays and Heavy Ions

Bonev

Kozubek

Krasavin

et al. 1990

International Journal of Radiation Biology

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Lambda-prophage induction in repair-deficient and wild-type E. coli strains by heavy ions and gamma-rays has been investigated. The dose dependence of the fraction of induced cells has been measured and its initial slope (lambda-induction potency) determined. The induction by gamma-rays was found to be more efficient in a polA-repair-deficient strain; the value of lambda-induction potency is zero in lexA- and recA- strains. The lambda-induction potency increased with LET for wild-type cells but remained constant in the case of polA- mutant cells. It is suggested that the DNA damage triggering the lambda-prophage induction in the case of ionizing radiation could be a type of DNA single-strand break with complex structure which cannot be repaired by fast repair processes, and which requires a substantial level of energy deposition for induction in a DNA molecule.

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Section: the Role Of Genotype In 2-prophage Inductionmentioning

confidence: 64%

“…The latter class of DNA damage can arise in one strand of DNA as `complex' ssb, which are not repairable by a fast polAdependent repair system in E . coli cells (Bridges and Mottershead 1972) . It can also arise in both DNA strands as dsb, which is a lethal event in E .…”

Section: The Probability Of A-prophage Induction Per Killed Cell P(d)mentioning

confidence: 99%

λ-prophage Induction in Repair-deficient and Wild TypeE. ColiStrains by γ-rays and Heavy Ions

Bonev

Kozubek

Krasavin

et al. 1990

International Journal of Radiation Biology

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“…UV doses of 10-30 J/m 2 were chosen as these were similar to the UV doses used in previous studies [30,31]. X-irradiation doses were also chosen based on precedent in the literature and are cited in Gray (Gy) [32,33]. CAP dose is presented as function of treatment time.…”

Section: Cap-mediated Dna Damage Causes Mutations At Sublethal Dosesmentioning

confidence: 99%

Assessment of mutations induced by cold atmospheric plasma jet treatment relative to known mutagens in Escherichia coli

et al. 2021

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The main bactericidal components of cold atmospheric plasma (CAP) are thought to be reactive oxygen and nitrogen species (RONS) and UV radiation, both of which have the capacity to cause DNA damage and mutations. Here, the mutagenic effects of CAP on Escherichia coli were assessed in comparison to X- and UV-irradiation. DNA damage and mutagenesis were screened for using a diffusion-based DNA fragmentation assay and modified Ames test respectively. Mutant colonies obtained from the latter were quantitated and sequenced. CAP was found to elicit a similar mutation spectrum to X-irradiation, that did not resemble that for UV implying that CAP produced RONS are more likely the mutagenic component of CAP. CAP treatment was also shown to promote resistance to the antibiotic ciprofloxacin. Our data suggest that CAP treatment has mutagenic effects that may have important phenotypic consequences.

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Mutagenesis

Crueger

1992

Biotechnology

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Gamma ray mutagenesis in a strain of Escherichia coli deficient in DNA polymerase I

Cited by 17 publications

References 20 publications

λ-prophage Induction in Repair-deficient and Wild TypeE. ColiStrains by γ-rays and Heavy Ions

λ-prophage Induction in Repair-deficient and Wild TypeE. ColiStrains by γ-rays and Heavy Ions

Assessment of mutations induced by cold atmospheric plasma jet treatment relative to known mutagens in Escherichia coli

Mutagenesis

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