Gamma﻿ ray mutants of oleaginous microalga Chlorella sp. KM504965 with enhanced biomass and lipid for biofuel production

Senthamilselvi, Dhandapani

doi:10.1007/s13399-022-02400-9

Cited by 11 publications

(4 citation statements)

References 72 publications

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“…This is another issue to be resolved in the future, as more detailed differences will be revealed by clarifying the genome-wide mode of mutagenesis. Furthermore, another study breeding Chlorella (microalgae) by gamma-ray irradiation reported that a strain that produces a 1.2 to 2.1 times higher amount of lipid than wild-type was obtained through irradiation with 800 Gy of gamma rays 23 . However, under those irradiation conditions, only 10% of the colonies survived.…”

Section: Discussionmentioning

confidence: 99%

Optimal conditions of algal breeding using neutral beam and applying it to breed Euglena gracilis strains with improved lipid accumulation

Imamura,

Yamada,

Takebe

et al. 2024

Sci Rep

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Microalgae are considered to be more useful and effective to use in biomass production than other photosynthesis organisms. However, microalgae need to be altered to acquire more desirable traits for the relevant purpose. Although neutron radiation is known to induce DNA mutations, there have been few studies on its application to microalgae, and the optimal relationship between irradiation intensity and mutation occurrence has not been established. In this study, using the unicellular red alga Cyanidioschyzon merolae as a model, we analyzed the relationship between the absorbed dose of two types of neutrons, high-energy (above 1 MeV) and thermal (around 25 meV) neutrons, and mutation occurrence while monitoring mutations in URA5.3 gene encoding UMP synthase. As a result, the highest mutational occurrence was observed when the cells were irradiated with 20 Gy of high-energy neutrons and 13 Gy of thermal neutrons. Using these optimal neutron irradiation conditions, we next attempted to improve the lipid accumulation of Euglena gracilis, which is a candidate strain for biofuel feedstock production. As a result, we obtained several strains with a maximum 1.3-fold increase in lipid accumulation compared with the wild-type. These results indicate that microalgae breeding by neutron irradiation is effective.

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Section: Discussionmentioning

confidence: 99%

Optimal conditions of algal breeding using neutral beam and applying it to breed Euglena gracilis strains with improved lipid accumulation

Imamura,

Yamada,

Takebe

et al. 2024

Sci Rep

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“…Moreover, gamma radioresistance occurs by changing physiological and metabolic processes in cells to form primary defense mechanisms in microalgae species [ 41 , 42 ]. Senthamilselvi [ 43 ] used Chlorella sp. to obtain mutants for increasing biomass and lipid production.…”

Section: Discussionmentioning

confidence: 99%

Microalgae Diversity in Interim Wet Storage of Spent Nuclear Fuel in Serpong, Indonesia

Sugoro

Pikoli

Rahayu

et al. 2022

IJERPH

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The water quality in the interim wet storage of spent fuel (ISSF) needs to be monitored due to its function as a radiation shield. Water in ISSF pools must be free from microorganisms such as microalgae that live in a radioactive environment. Moreover, particular microalgae are capable of causing corrosion to stainless steel, which is a component of ISSF. Therefore, this study aims to determine the diversity of microalgae in the ISSF and those living in a radioactive environment, which cause corrosion. The microalgae were detected using the diversity and Palmer indices. The sampling of microalgae water was carried out by vertical filtration method at eight sites of ISSF. The results show that the diversity of microalgae (H′) was low due to radiation exposure in pool water, hence, only specific species can survive. The evenness (J′) of the microalgae was low, causing a high dominance index (C) value. Furthermore, the dominating species, namely, Chlorella sp. (Chlorophyceae), needs to be monitored because it has gamma radioresistance capabilities and can cause the corrosion of stainless steel.

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“…Moreover, Sarayloo et al [ 115 ] used a combination of UV radiation and EMS to mutate C. vulgaris and its isolated mutants exhibited 67% increased lipid content and 35% increased biomass than those of the wildtype ( Table 1 ). In a different study, physically induced random mutagenesis using gamma rays led to Chlorella mutants suitable for biodiesel production ( Table 1 ) [ 113 ].…”

Section: Strategies For Microalgal Strain Improvementmentioning

confidence: 99%

Random Mutagenesis as a Promising Tool for Microalgal Strain Improvement towards Industrial Production

Trovão

Schüler

Machado³

et al. 2022

Marine Drugs

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Microalgae have become a promising novel and sustainable feedstock for meeting the rising demand for food and feed. However, microalgae-based products are currently hindered by high production costs. One major reason for this is that commonly cultivated wildtype strains do not possess the robustness and productivity required for successful industrial production. Several strain improvement technologies have been developed towards creating more stress tolerant and productive strains. While classical methods of forward genetics have been extensively used to determine gene function of randomly generated mutants, reverse genetics has been explored to generate specific mutations and target phenotypes. Site-directed mutagenesis can be accomplished by employing different gene editing tools, which enable the generation of tailor-made genotypes. Nevertheless, strategies promoting the selection of randomly generated mutants avoid the introduction of foreign genetic material. In this paper, we review different microalgal strain improvement approaches and their applications, with a primary focus on random mutagenesis. Current challenges hampering strain improvement, selection, and commercialization will be discussed. The combination of these approaches with high-throughput technologies, such as fluorescence-activated cell sorting, as tools to select the most promising mutants, will also be discussed.

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Gamma ray mutants of oleaginous microalga Chlorella sp. KM504965 with enhanced biomass and lipid for biofuel production

Cited by 11 publications

References 72 publications

Optimal conditions of algal breeding using neutral beam and applying it to breed Euglena gracilis strains with improved lipid accumulation

Optimal conditions of algal breeding using neutral beam and applying it to breed Euglena gracilis strains with improved lipid accumulation

Microalgae Diversity in Interim Wet Storage of Spent Nuclear Fuel in Serpong, Indonesia

Random Mutagenesis as a Promising Tool for Microalgal Strain Improvement towards Industrial Production

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Gamma﻿ ray mutants of oleaginous microalga Chlorella sp. KM504965 with enhanced biomass and lipid for biofuel production

Cited by 11 publications

References 72 publications

Optimal conditions of algal breeding using neutral beam and applying it to breed Euglena gracilis strains with improved lipid accumulation

Optimal conditions of algal breeding using neutral beam and applying it to breed Euglena gracilis strains with improved lipid accumulation

Microalgae Diversity in Interim Wet Storage of Spent Nuclear Fuel in Serpong, Indonesia

Random Mutagenesis as a Promising Tool for Microalgal Strain Improvement towards Industrial Production

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Gamma ray mutants of oleaginous microalga Chlorella sp. KM504965 with enhanced biomass and lipid for biofuel production