Gancao Nurish-Yin Decoction medicated serum inhibits growth and migration of ovarian cancer cells: Network pharmacology-based analysis and biological validation

Pan, Rongbin; Chen, Yanjuan; Nandakumar, Kutty Selva; Chen, Yong

doi:10.1016/j.prmcm.2022.100062

Cited by 3 publications

(7 citation statements)

References 21 publications

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“…Gancao nourishing yin (GCNY) decoction is a Chinese herbal formula designed to tonify yin , while also promoting yang by body regulation. Previous studies validate its potential indications in age-related disorders such as constipation and ovarian cancer ( Pan et al, 2022a ), and the invention has been patented (ZL 2019 1 0843519.9 and ZL 2021 1 0515336.1) in China. Changes in metabolic profiles in the liver of hTNF-α transgenic mice were also observed ( Pan et al, 2022b ).…”

Section: Introductionmentioning

confidence: 77%

“…For example, Nrf2 is rather than promoted but declined synergistically to its multiple signaling pathway-related molecules. Another reason is hundreds of orally absorbable ingredients and the corresponding targets identified in the formula using the network pharmacology approach ( Pan et al, 2022a ). Much evidence believes that indirect and overall body regulation to the targets of a disease is a more reasonable explanation to how TCM works, and as being absorbed and circulated in vivo , the medicated serum contains the impact of a herb formula within the serum that provides accessibility for GCNY to verify its effects in vitro.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, medicated serum is currently a popular and recognized protocol to conduct in vitro study on TCM formula. Different to chemical drugs or a single extract from herbal medicine, the traditional formula such as GCNY is likely to possess hundreds of chemical composition with oral bioavailability (OB) ≥30% and drug-like properties (DL) ≥0.18 ( Pan et al, 2022a ). Compositions of the formula digested and absorbed by rats, then interacted with body to regulate neuro-endocrine immune system (NEI), and then applied to the cells.…”

Section: Discussionmentioning

confidence: 99%

“…The GCNY-medicated serum and its control serum were stored in nitrogen canister and applied to this research after rewarming in room temperature. The detailed serum preparation method was shown in our previously published paper ( Pan et al, 2022a ). Briefly, SPF grade, healthy SD male rats purchased from Animal Experimental Center of Southern Medical University [production license number: SCXK (Yue) 2016-0041] were assigned into 2 groups to produce GCNY-medicated serum and its control serum.…”

Section: Methodsmentioning

confidence: 99%

“…Changes in metabolic profiles in the liver of hTNF-α transgenic mice were also observed ( Pan et al, 2022b ). It not only shows anti-inflammatory functions, but can also improve the health status (reflected by kidney Qi and blood of TCM theories) via the presence of herbal ingredients of licorice, ginseng, dandelion, Monk fruit, polygonatum, jujube, and ginger ( Pan et al, 2022a ). Based on these TCM theories and experimental results observed, we believe that the oxidation process, which shows profound interactions with metabolism and inflammation ( Bekeschus et al, 2017 ), shall be proposed as the fundamental mechanism explaining how GCNY works.…”

Section: Introductionmentioning

confidence: 99%

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Chinese herbal formula (GCNY)-medicated serum alleviates peroxidation induced by H2O2 in human microglial cells

et al. 2022

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Traditional Chinese herbal medicine aiming at nourishing yin formed a distinctive school of thought in history to achieve anti-aging and longevity. In the formula Gancao nourishing yin (GCNY) decoction, all of the ingredients show antioxidant properties. However, in real clinical practice, extractions of herbs are rarely applied alone but are prescribed as the integrated formula. To investigate whether GCNY possesses anti-oxidation potential, we applied GCNY to treat rats to acquire medicated serum, which was then added on H2O2 (200 μM)-modeled human microglial cell line HMC-3 in comparison with its control serum. The results revealed that GCNY-medicated serum decreased reactive oxygen species (ROS) levels. Inflammatory cytokines such as pNF-κB p65 (ser536) and IL-6 were also decreased. Nrf2 and its pathway-related molecules, such as HO1, ABCC2, GLCM, ME1, NQO1, and TKT, were activated by H2O2 modeling while declined by treating with GCNY-medicated serum, which indicated attenuated oxidative stress of GCNY. Furthermore, mRNA-seq analysis showed 58 differential expressed genes (DEGs), which were enriched in pathways including antigen processing and presentation, longevity regulation, oxidative phosphorylation, and Parkinson’s disease progression. DEGs that were downregulated by H2O2 modeling but upregulated by GCNY treatment include CENPF, MKI67, PRR11, and TOP2A. Those targets were reported to be associated with the cell cycle and cell proliferation and belong to the category of growth factor genes. In conclusion, this study verified anti-oxidation effects of GCNY and indicated its promising application for cognitive degeneration and aging-related disorders.

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Section: Introductionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Chinese herbal formula (GCNY)-medicated serum alleviates peroxidation induced by H2O2 in human microglial cells

et al. 2022

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Gancao Nourishing-Yin decoction combined with methotrexate in treatment of aging CIA mice: a study based on DIA proteomic analysis

et al. 2023

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Background Elderly rheumatoid arthritis (ERA) population faces multiple treatment dilemma. Here we aim to investigate if Gancao Nourishing-Yin decoction (GCNY) added to methotrexate (MTX) exhibit better effects in an ERA mice model. Methods ERA mice model was established by adding D-galactose (Dgal) to collagen-induced arthritis (CIA) mice. The model was then assigned into control group (CIA + Dgal), MTX treatment group (MTX), GCNY treatment group (GCNY), and integrative treatment group (MTX + GCNY). Pathological scoring was performed to evaluate the severity between the groups. Proteomic analysis was applied to investigate the secretory phenotype of the ERA mouse model and the underlying mechanism of GCNY, MTX and their combination. Representative cytokines related to proteomic results were further validated by ELISAs. Results CIA + Dgal mice showed more aggressive joints damage than the CIA mice. Besides changes in the inflammatory pathway such as Pi3k-Akt signaling pathway in both model, differential expressed proteins (DEPs) indicated metabolism-related pathways were more obvious in CIA + Dgal mice. Low-dose MTX failed to show pathological improvement in CIA + Dgal mice, while GCNY improved joints damage significantly. Besides down-regulated inflammation-related targets, GCNY-regulated DEPs (such as Apoc1 ~ 3, Grk2 and Creb3l3) were broadly enriched in metabolism-related pathways. MTX + GCNY showed the best therapeutic effect, and the DEPs enriched in a variety of inflammatory,metabolism and osteoclast differentiation signaling pathway. Notably, MTX + GCNY treatment up-regulated Dhfr, Cbr1, Shmt1 involved in folic acid biosynthesis and anti-folate resistance pathways indicated a coincidence synergic action. ELISAs confirmed CPR and Akt that elevated in CIA + Dgal mice were significantly ameliorated by treatments, and adding on GCNY elevated folic acid levels and its regulator Dhfr. Conclusion Aging aggravated joints damage in CIA, which probably due to metabolic changes rather than more severe inflammation. GCNY showed significant effects in the ERA mice model especially when integrated with MTX to obtain a synergic action.

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α-Mangostin-phytosomes as a plausible nano-vesicular approach for enhancing cytotoxic activity on SKOV-3 ovarian cancer cells

Alamoudi,

Badr-Eldin,

Ahmed

et al. 2024

Futur J Pharm Sci

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Background α-Mangostin is a major xanthone in Garcinia mangostana L. (Clusiaceae) pericarps. It has promising anti-proliferative potential in different cancer cells; however, it has poor oral bioavailability. Phytosomes are used as a novel nano-based drug delivery system. The aim of this research was to enhance the anti-proliferative potency of α-mangostin by formulating it as α-mangostin-phytosome (α-M-PTMs) and assessing its impact on SKOV-3 ovarian cancer cells in comparison to pure α-mangostin. Results The size and entrapment efficiency of the proposed formulation were optimized using Box–Behnken statistics. The optimized formula was characterized using transmission electron microscope. The binding of α-mangostin to phospholipids was confirmed using Fourier-transform infrared (FTIR) spectroscopy. The optimized α-mangostin-phytosomes formula exhibited enhanced anti-proliferative activity with reference to raw α-mangostin. This was further substantiated by assessing the cell cycle phases that indicated an accumulation of SKOV-3 cells in the sub-G1 phase. Annexin-V staining revealed enhanced apoptotic activity in α-mangostin-phytosome-treated cells. This was associated with upregulation of CASP3 (Caspase-3), BAX (BCL2 Associated X, Apoptosis Regulator) and TP53 as well as down-regulation of BCL2 mRNA (B-Cell Leukemia/Lymphoma 2). Moreover, our data indicated enhanced ROS (Reactive oxygen species) production, cytochrome-C release, and disturbed MMP (mitochondrial membrane potential). Conclusion Encapsulation of α-mangostin in a phytosome nano-formula enhances its anti-proliferative effects in SKOV-3 cells via, at least in part, inducing mitochondrial apoptotic cell death.

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Gancao Nurish-Yin Decoction medicated serum inhibits growth and migration of ovarian cancer cells: Network pharmacology-based analysis and biological validation

Cited by 3 publications

References 21 publications

Chinese herbal formula (GCNY)-medicated serum alleviates peroxidation induced by H2O2 in human microglial cells

Chinese herbal formula (GCNY)-medicated serum alleviates peroxidation induced by H2O2 in human microglial cells

Gancao Nourishing-Yin decoction combined with methotrexate in treatment of aging CIA mice: a study based on DIA proteomic analysis

α-Mangostin-phytosomes as a plausible nano-vesicular approach for enhancing cytotoxic activity on SKOV-3 ovarian cancer cells

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