Ganglion cell types of the turtle retina that project to the optic tectum: Intracellular HRP injections of retrogradely, rhodamine-marked cell bodies

Guiloff, Gloria D.; Kolb, Helga

doi:10.1017/s0952523800005046

Cited by 13 publications

(22 citation statements)

References 49 publications

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“…The irregular, dichotomous branching pattern with spine-bearing major dendrites lying in 53 and the possession of axon-like processes coming from either the cell body or a major dendrite are all features of similarity between the SP-IR cell described in this paper and the thorny type 2 cells of Mariani (1990) and of Kolb et al (1992; Fig. 9).…”

Section: Discussionmentioning

confidence: 51%

“…SP stained only the cell body and partially into the major dendrites of these ganglion cells, so we could tell only that the dendrites most likely stratified in S3. Based on these incomplete morphological criteria, we suggest that the SP-IR ganglion cell revealed in this study most resembles G21 of the Golgi study (Kolb et al, 1992). This is a wide-field cell with a spindle-shaped cell body, approximately 22 pm in diameter, typically producing two major thick dendrites from either side of the cell body.…”

Section: Discussionmentioning

confidence: 56%

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Substance P‐immunoreactive neurons in the human retina

Cuenca

Juan

Kolb

1995

J of Comparative Neurology

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Substance P (SP) is a neuropeptide that acts as a neurotransmitter or a neuromodulator in the retina. The aim of this study was to identify the type(s) and the distribution of the SP-immunoreactive (SP-IR) cells in the human retina. We have used an antiserum to SP to immunostain neurons in postmortem human retinae. Immunostained retinae were processed with the avidin-biotin complex (ABC) to visualize the cells either whole mounted in glycerol or embedded in plastic. Some retinae were also sectioned at 20 microns in order to obtain radial views of stained cells. SP-IR amacrine cells stain intensely and appear to be of a single type in the human retina. They are large-field cells with large cell bodies (16 microns diameter) lying in normal or displaced positions on either side of the inner plexiform layer (IPL). Their sturdy, spiny, and appendage-bearing dendrites stratify in stratum 3 (S3) of the IPL, where many overlapping, fine dendrites intermingle to form a plexus of stained processes. Either cell bodies or primary dendrites emit an "axon-like" process that, typically, divides into two long, fine processes, which run in opposite directions for hundreds of micrometers in S5 and S3 before disappearing as distinct entities in the stained plexus in S3. Long, fine dendrites also pass from the dendritic plexus to run in S5 and down to the nerve fiber layer to end as large varicosities at blood vessel walls. In addition, fine processes are emitted from the dendritic plexus that runs in S1, and some pass up to the outer plexiform layer (OPL) to run therein for short distances. The SP-IR amacrine cell has many similarities to the thorny, type 2 amacrine cells described from Golgi studies. In addition to the SP-IR amacrine cells, a presumed ganglion cell type is faintly immunoreactive. Its 20-22 microns cell body gives rise to a radiate, sparsely branched, wide-spreading dendritic tree running in S3. Its dendrites and cell body become enveloped by the more intensely SP-IR processes and boutons from the SP-IR amacrine cell type. The SP-IR ganglion cell type most resembles G21 from a Golgi study.

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Section: Discussionmentioning

confidence: 51%

Section: Discussionmentioning

confidence: 56%

Substance P‐immunoreactive neurons in the human retina

Cuenca

Juan

Kolb

1995

J of Comparative Neurology

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“…

ABSTRACTTwo well-stained, horseradish peroxidase-filled varieties of putative ON-OFF directionally selective ganglion cells, G14a and G15, that project to the dorsolateral optic tectum (Guiloff and Kolb [1992a] Vis. Neurosci.

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confidence: 99%

“…Twenty-four different types of ganglion cell are found in the retinas of the two species of turtles Pseudemys scriptu and Mauremys caspica (Kolb, 1982;Kolb et al, 1988;Guiloff and Kolb, 1992a;Ammermiiller et al, 1991Ammermiiller et al, , 1994Ammermuller and Kolb, 1994). They have been classified on specific criteria such as soma size, dendritic field size, dendritic morphology, and stratification levels in the inner plexiform layer (IPL).…”

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confidence: 99%

“…Recently, we have shown by retrograde tracing studies combined with intracellular horseradish peroxidase (HRP) staining (Guiloff et al, 1989;Guiloff and Kolb, 1992a) that most morphological ganglion cell types in the turtle project to the dorsolateral optic tectum. Two putative directionally selective ganglion cell types amongst them have been chosen here to be analyzed by EM.…”

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confidence: 99%

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Ultrastructural and immunocytochemical analysis of the circuitry of two putative directionally selective ganglion cells in turtle retina

Guiloff

Kolb

1994

J of Comparative Neurology

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Two well-stained, horseradish peroxidase-filled varieties of putative ON-OFF directionally selective ganglion cells, G14a and G15, that project to the dorsolateral optic tectum (Guiloff and Kolb [1992a] Vis. Neurosci. 8:295-313) were studied qualitatively and quantitatively. Both were bistratified ganglion cells with one tier of dendrites in the OFF sublamina and the other in the ON sublamina of the inner plexiform layer (IPL). The cells were serially sectioned and examined for synaptic inputs by electron microscopy. Portions of the dendritic trees were also analyzed after postembedding immunocytochemistry for neurotransmitter candidates gamma aminobutyric acid (GABA), glycine, choline acetyltransferase (ChAT), and glutamate in presynaptic neurons. Both G14a and G15 are dominated by amacrine cell inputs and have only minor bipolar cell involvement. Probably at least two different types of bipolar cell are presynaptic. Both ganglion cells receive some GABA-positive (GABA+) amacrine inputs and G14a receives ChAT+ amacrine inputs. Glycine+ and glutamate+ inputs could not be detected in either cell. The GABA+ inputs appeared to be regionally arranged in the dendritic trees. The general distribution of amacrine and bipolar inputs to the two tiers of dendrites in both cell types appeared to be asymmetrical, both along the radial extent of the dendritic trees and within the depth of the IPL. Our data support some aspects of the current models for directional selectivity. We suggest candidate bipolar and amacrine cells that could have input to these ganglion cells. Since many of the putative presynaptic amacrine cells coincide with directionally selective types recorded and stained by other authors, we propose that in turtle retina directional selectivity arises in neurons presynaptic to the ganglion cells.

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Colocalization of choline acetyltransferase and ?-aminobutyric acid in the developing and adult turtle retinas

Nguyen

Grzywacz

2000

J. Comp. Neurol.

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Acetylcholine and ␥ -aminobutyric acid (GABA) are putative neurotransmitters in the adult vertebrate retina. In this study, cells that coexpress choline acetyltransferase (ChAT) and GABA or glutamic acid decarboxylase (GAD) were investigated in turtle retinas from stage 14 (S14) to adulthood by using a double-labeling immunofluorescence technique. ChAT immunoreactivity was observed at S15 and included not only the presumptive starburst cholinergic amacrine cells but also a population in the ganglion cell layer (GCL) that expressed ChAT transiently during the embryonic stages (see the accompanying paper:

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Ganglion cell types of the turtle retina that project to the optic tectum: Intracellular HRP injections of retrogradely, rhodamine-marked cell bodies

Cited by 13 publications

References 49 publications

Substance P‐immunoreactive neurons in the human retina

Substance P‐immunoreactive neurons in the human retina

Ultrastructural and immunocytochemical analysis of the circuitry of two putative directionally selective ganglion cells in turtle retina

Colocalization of choline acetyltransferase and ?-aminobutyric acid in the developing and adult turtle retinas

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