Ganglioside GM1 Elevation in DBA/2 Mouse Embryos

Bouvier, Joseph D.; Seyfried, Thomas N.

doi:10.1159/000111841

Cited by 7 publications

(2 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, it is thought that the GSL expression patterns in the developing mouse brains are regulated mainly by glycosyltransferases, but not by glycosidases. On the other hand, a previous study suggested that the GM1 amount increased in embryos of DBA/2 mice, exhibiting reduced β‐galactosidase activity (Bouvier and Seyfried 1990). We do not have a satisfactory explanation as to this anomaly.…”

Section: Discussionmentioning

confidence: 93%

Developmental changes of glycosphingolipids and expression of glycogenes in mouse brains

Ngamukote

Yanagisawa

Ariga

et al. 2007

Journal of Neurochemistry

186

203

View full text Add to dashboard Cite

Glycosphingolipids (GSLs) and their sialic acid-containing derivatives, gangliosides, are important cellular components and are abundant in the nervous system. They are known to undergo dramatic changes during brain development. However, knowledge on the mechanisms underlying their qualitative and qualitative changes is still fragmentary. In this investigation, we have provided a detailed study on the developmental changes of the expression patterns of GSLs, GM3, GM1, GD3, GD1a, GD2, GD1b, GT1b, GQ1b, A2B5 antigens (c-series gangliosides such as GT3 and GQ1c), Chol-1a (GT1aa and GQ1ba), glucosylceramide, galactosylceramide (O1 antigen), sulfatide (O4 antigen), stage-specific embryonic antigen-1 (Lewis x) glycolipids, and human natural killer-1 glycolipid (sulfoglucuronosyl paragloboside) in developing mouse brains [embryonic day 12 (E12) to adult]. In E12-E14 brains, GD3 was a predominant ganglioside. After E16, the concentrations of GD3 and GM3 markedly decreased, and the concentrations of a-series gangliosides, such as GD1a, increased. GT3, glucosylceramide, and stagespecific embryonic antigen-1 were expressed in embryonic brains. Human natural killer-1 glycolipid was expressed transiently in embryonic brains. On the other hand, Chol-1a, galactosylceramide, and sulfatide were exclusively found after birth. To provide a better understanding of the metabolic basis for these changes, we analyzed glycogene expression patterns in the developing brains and found that GSL expression is regulated primarily by glycosyltransferases, and not by glycosidases. In parallel studies using primary neural precursor cells in culture as a tool for studying developmental events, dramatic changes in ganglioside and glycosyltransferase gene expression were also detected in neurons induced to differentiate from neural precursor cells, including the expression of GD3, followed by up-regulation of complex a-and b-series gangliosides. These changes in cell culture systems resemble that occurring in brain. We conclude that the dramatic changes in GSL pattern and content can serve as useful markers in neural development and that these changes are regulated primarily at the level of glycosyltransferase gene expression.

show abstract

Section: Discussionmentioning

confidence: 93%

Developmental changes of glycosphingolipids and expression of glycogenes in mouse brains

Ngamukote

Yanagisawa

Ariga

et al. 2007

Journal of Neurochemistry

186

203

View full text Add to dashboard Cite

show abstract

“…In human and mouse, the two lysosomal ␤-galactosidases, galactosylceramidase (GALC) and acid ␤-galactosidase (BGAL), share substrate specificity to degrade lactosylceramide (Tanaka and Suzuki, 1976). It is known that tissue activities of acid ␤-galactosidase vary in different strains of mice (Felton et al, 1974;Bouvier and Seyfried, 1990). This factor might be responsible for lactosylceramide accumulation in the brain of twitcher mice on the mixed background.…”

Section: Discussionmentioning

confidence: 99%

Genetic background markedly influences vulnerability of the hippocampal neuronal organization in the “twitcher” mouse model of globoid cell leukodystrophy

Tominaga

Matsuda

Kido

et al. 2004

J of Neuroscience Research

View full text Add to dashboard Cite

The twitcher mouse is well known as a naturally occurring authentic mouse model of human globoid cell leukodystrophy (GLD; Krabbe disease) due to genetic deficiency of lysosomal galactosylceramidase. The twitcher mice used most commonly are on the C57BL/6J background. We generated twitcher mice that were on the mixed background of C57BL/6J and 129SvEv, the standard strain for production of targeted mutations. Twitcher mice on the mixed background were smaller and had a shorter lifespan than were those on the C57BL/6J background. Many twitcher mice on the mixed background developed generalized seizures around 30 days that were never seen in twitcher mice on the C57BL/6J background. Neuropathologically, although the degree of the typical demyelination with infiltration of macrophages was similar in the central and peripheral nervous systems, in both strains, marked neuronal cell death was observed only in twitcher mice on the mixed background. In the hippocampus, the neuronal cell death occurred prominently in the CA3 region in contrast to the relatively well-preserved CA1 and CA2 areas. This neuropathology has never been seen in twitcher mice on the C57BL/6J background. Biochemically, the brain of twitcher mice on the mixed background showed much greater accumulation of lactosylceramide. Genetic background must be carefully taken into consideration when phenotype of mutant mice is evaluated, particularly because most targeted mutants are initially on a mixed genetic background and gradually moved to a pure background. These findings also suggest an intriguing possibility of important function of some sphingolipids in the hippocampal neuronal organization and maintenance.

show abstract

Organization of the mouse acid β-galactosidase gene

Nanba¹,

Suzuki²

1991

Biochemical and Biophysical Research Communications

View full text Add to dashboard Cite

Ganglioside GM1 Elevation in DBA/2 Mouse Embryos

Cited by 7 publications

References 33 publications

Developmental changes of glycosphingolipids and expression of glycogenes in mouse brains

Developmental changes of glycosphingolipids and expression of glycogenes in mouse brains

Genetic background markedly influences vulnerability of the hippocampal neuronal organization in the “twitcher” mouse model of globoid cell leukodystrophy

Organization of the mouse acid β-galactosidase gene

Contact Info

Product

Resources

About