Ganglioside GM1 influences the proliferation rate of mouse induced pluripotent stem cells

Ryu, Jae‐Sung; Chang, Kyu‐Tae; Lee, Ju-Taek; Lim, Malg-Um; Min, Hyun-Ki; Lee, Su-Bin; Moussavou, Gislain; Kim, Sun-Uk; Kim, Jisu; Ko, Kinarm; Ko, Kisung; Hwang, Ki Won; Jeong, Eun-Jeong; Lee, Jeong-Woong; Choo, Young‐Kug

doi:10.5483/bmbrep.2012.45.12.138

Cited by 5 publications

(7 citation statements)

References 35 publications

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“…Ganglioside (GD3 d18:1/24:1 m/z 1592.881) was not observed in the differentiated stem cells, but is seen in the control cells. Gangliosides are generally responsible for proliferation and transmembrane signaling [61]. They reside in the lipid raft of the membrane [62] (Figure 7f) and are found to be lower with adipogenesis [63][64][65], which is consistent with our observation.…”

Section: Resultssupporting

confidence: 91%

Multimodal Label-free Monitoring of Adipogenic Stem Cell Differentiation using Endogenous Optical Biomarkers

Mehta

Shaik

Prasad

et al. 2020

Preprint

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Stem cell-based therapies carry significant promise for treating human diseases. However, clinical translation of stem cell transplants for effective therapy requires precise non-destructive evaluation of the purity of stem cells with high sensitivity (< 0.001% of the number of cells). Here, we report a novel methodology using hyperspectral imaging (HSI) combined with spectral angle mapping (SAM)-based machine learning analysis to distinguish differentiating human adipose derived stem cells (hASCs) from control stem cells. The spectral signature of adipogenesis generated by the HSI method enabled identification of differentiated cells at single cell resolution. The label-free HSI method was compared with the standard methods such as Oil Red O staining, fluorescence microscopy, and qPCR that are routinely used to evaluate adipogenic differentiation of hASCs. Further, we performed Raman microscopy and multiphoton-based metabolic imaging to provide complimentary information for the functional imaging of the hASCs. Finally, the HSI method was validated using matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) imaging of the stem cells. The study presented here demonstrates that multimodal imaging methods enable label-free identification of stem cell differentiation with high spatial and chemical resolution. This could provide a powerful tool to assess the safety and efficacy of stem cell-based regenerative therapies.

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Section: Resultssupporting

confidence: 91%

Multimodal Label-free Monitoring of Adipogenic Stem Cell Differentiation using Endogenous Optical Biomarkers

Mehta

Shaik

Prasad

et al. 2020

Preprint

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“…2). Other investigators also reported that exogenously added gangliosides exhibited stimulatory effects on cellular proliferation of induced pluripotent stem [47] and vascular smooth muscle cells [48]. We have reported NSCs express GD3 as a predominant ganglioside [26,49, 50] and it is co-localized with EGFR to regulate cellular proliferation induced by EGF (unpublished data).…”

Section: Discussionmentioning

confidence: 60%

Effects of Amyloid β-Peptides and Gangliosides on Mouse Neural Stem Cells

et al. 2013

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The interaction of amyloid β-proteins (Aβs) with membrane lipids has been postulated as an early event in Aβ fibril formation in Alzheimer’s disease. We evaluated the effects of several putative bioactive Aβs and gangliosides on neural stem cells (NSCs) isolated from embryonic mouse brains or the subventricular zone of adult mouse brains. Incubation of the isolated NSCs with soluble Aβ1–40 alone did not cause any change in the number of NSCs, but soluble Aβ1–42 increased their number. Aggregated Aβ1–40 and Aβ1–42 increased the number of NSCs but soluble and aggregated Aβ25–35 decreased the number. Soluble Aβ1–40 and Aβ1–42 did not affect the number of apoptotic cells but aggregated Aβ1–40 and Aβ1–42 did. When NSCs were treated with a combination of GM1 or GD3 and soluble Aβ1–42, cell proliferation was enhanced, indicating that both GM1 and GD3 as well as Aβs are involved in promoting cell proliferation and survival of NSCs. These observations suggest the potential of beneficial effects of using gangliosides and Aβs for promoting NSC proliferation.

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“…OG2 mouse embryonic fibroblast (MEF) and mESCs produce GM3, GM1, and GD1a, but GM1 is not found in OG2 MEF-derived iPSCs (Fig. 3B) (39). Analysis of the cell proliferation rate in OG2 mESCs and iPSCs revealed that iPSCs have a lower proliferation than that of mESCs.…”

Section: Expression Patterns Of Gangliosides In Mouse Pluripotent Stementioning

confidence: 96%

“…In E14 and Oct-4 promoter-EGFP (OG2) mESCs, small amounts of a-series gangliosides, GM3, GM1 and GD1a, were detected by high-performance thin-layer chromatography and immunocytochemistry analysis (38,39). Furthermore, in TC-1 mESCs, only glucosylceramide and lactosylceramide were present (40); however, J1 mESCs contained GM3, GM1, and GD3 ( Fig.…”

Section: Expression Patterns Of Gangliosides In Mouse Pluripotent Stementioning

confidence: 99%

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Roles of gangliosides in the differentiation of mouse pluripotent stem cells to neural stem cells and neural cells

Ryu

et al. 2017

Molecular Medicine Reports

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Glycosphingolipids are important components of the outer layer of the plasma membrane in the majority of eukaryotic cells. Specifically, gangliosides are sialic acid‑containing glycosphingolipids that participate in cell‑cell recognition, adhesion, proliferation, differentiation and signal transduction, and are integral components of cell surface microdomains and lipid rafts. Stem cells are defined functionally as cells that have the capacity to self‑renewal and differentiate to generate various cell types. Due to different synthesis patterns and locations of gangliosides, they have been used as molecular markers of stem cells. The current review describes the presence of gangliosides in various types of mouse stem cells, including pluripotent stem cells (embryonic stem cells and induced pluripotent stem cells) and neural stem cells, and the functional roles of gangliosides in various processes, including cell proliferation and neural differentiation. Thus, this review will aid the understanding of gangliosides patterns and functions in mouse stem cells, and outline markers for the identification of stem cells.

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Ganglioside GM1 influences the proliferation rate of mouse induced pluripotent stem cells

Cited by 5 publications

References 35 publications

Multimodal Label-free Monitoring of Adipogenic Stem Cell Differentiation using Endogenous Optical Biomarkers

Multimodal Label-free Monitoring of Adipogenic Stem Cell Differentiation using Endogenous Optical Biomarkers

Effects of Amyloid β-Peptides and Gangliosides on Mouse Neural Stem Cells

Roles of gangliosides in the differentiation of mouse pluripotent stem cells to neural stem cells and neural cells

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