Ganglioside Profiles in Human Gliomas: Quantification by Microbore High Performance Liquid Chromatography and Correlation to Histomorphology and Grading

Wagener, Raimund; Röhn, Gabriele; Schillinger, Gerhard; Schröder, R.; Kobbe, Birgit; Ernestus, Ralf‐Ingo

doi:10.1007/s007010050439

Cited by 32 publications

(27 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…GBM tumors are known to either overexpress or synthesize modified forms of GD3, GD2, GM1, GM2, 3Viso-LM1, and 3V6V-isoLD1 (8,10,53,60). Others have reported that gangliosides (mostly GD3 and GM3) are shed into the serum of GBM patients (8,61).…”

Section: Discussionmentioning

confidence: 99%

Glioblastomas Induce T-Lymphocyte Death by Two Distinct Pathways Involving Gangliosides and CD70

Chahlavi¹,

et al. 2005

View full text Add to dashboard Cite

Here we report that glioblastoma multiforme (GBM) mediates immunosuppression by promoting T-cell death via tumorassociated CD70 and gangliosides that act through receptordependent and receptor-independent pathways, respectively. GBM lines cocultured with T cells induced lymphocyte death. The GBM lines were characterized for their expression of CD70, Fas ligand (FasL), and tumor necrosis factor-A (TNF-A), and the possible participation of those molecules in T-cell killing was assessed by doing GBM/T cell cocultures in the presence of anti-CD70 antibodies, Fas fusion proteins, or anti-TNF-A antibodies. CD70 but not TNF-A or FasL is responsible for initiating T-cell death via the receptor-dependent pathway. Of the four GBM cell lines that induced T-cell death, three highly expressed CD70. Two nonapoptogenic GBM lines (CCF3 and U138), on the other hand, had only minimally detectable CD70 expression. Blocking experiments with the anti-CD70 antibody confirmed that elevated CD70 levels were involved in the apoptogenicity of the three GBM lines expressing that molecule. Gangliosides were found to participate in the induction of T-cell apoptosis, because the glucosylceramide synthase inhibitor (PPPP) significantly reduced the abilities of all four apoptogenic lines to kill the lymphocytes. Highperformance liquid chromatography (HPLC) and mass spectroscopy revealed that GM2, GM2-like gangliosides, and GD1a were synthesized in abundance by all four apoptogenic GBM lines but not by the two GBMs lacking activity. Furthermore, gangliosides isolated from GBM lines as well as HPLC fractions containing GM2 and GD1a were directly apoptogenic for T cells. Our results indicate that CD70 and gangliosides are both products synthesized by GBMs that may be key mediators of T-cell apoptosis and likely contribute to the T-cell dysfunction observed within the tumor microenvironment. (Cancer Res 2005; 65(12): 5428-38)

show abstract

Section: Discussionmentioning

confidence: 99%

Glioblastomas Induce T-Lymphocyte Death by Two Distinct Pathways Involving Gangliosides and CD70

Chahlavi¹,

et al. 2005

View full text Add to dashboard Cite

show abstract

“…In turn, neuroectoderm-derived tumors such as neuroblastomas and melanomas and T-cell leukemias express ganglioside GD3 and/or GD2 that have been known as cancerassociated antigens (6,7). Although several reports showed expression of GD3 and GD2 in human gliomas, the roles of these gangliosides have not been demonstrated to date (8,9).…”

mentioning

confidence: 97%

Ganglioside GD3 Enhances Invasiveness of Gliomas by Forming a Complex with Platelet-derived Growth Factor Receptor α and Yes Kinase

Ohkawa

Momota

Kato

et al. 2015

Journal of Biological Chemistry

View full text Add to dashboard Cite

Background: Roles of GD3 in gliomas are not well understood. Results: PDGF receptor ␣ was identified as a GD3-associated molecule by enzyme-mediated activation of radical sources and mass spectrometry, and its association with GD3 and Yes leads to increased invasiveness. Conclusion: GD3 enhances invasiveness by forming a molecular complex. Significance: GD3/PDGF receptor ␣⅐Yes complex is a potential target for glioma therapy.

show abstract

“…Moreover, the results are validated by previous reports on the characterization of lipids from gliomas, each involving initial lipid extraction followed by either HPLC or HPTLC separation. [16,19,20] A follow up biomarker discovery phase will require increasing the sample size to derive and validate classification models. Multiple sampling sites with varying tumor cell density would also contribute to minimizing the effect of a lack of true non-tumor controls inherent to the study of brain diseases.…”

mentioning

confidence: 99%

Discrimination of Human Astrocytoma Subtypes by Lipid Analysis Using Desorption Electrospray Ionization Imaging Mass Spectrometry

et al. 2010

View full text Add to dashboard Cite

Ganglioside Profiles in Human Gliomas: Quantification by Microbore High Performance Liquid Chromatography and Correlation to Histomorphology and Grading

Cited by 32 publications

References 23 publications

Glioblastomas Induce T-Lymphocyte Death by Two Distinct Pathways Involving Gangliosides and CD70

Glioblastomas Induce T-Lymphocyte Death by Two Distinct Pathways Involving Gangliosides and CD70

Ganglioside GD3 Enhances Invasiveness of Gliomas by Forming a Complex with Platelet-derived Growth Factor Receptor α and Yes Kinase

Discrimination of Human Astrocytoma Subtypes by Lipid Analysis Using Desorption Electrospray Ionization Imaging Mass Spectrometry

Contact Info

Product

Resources

About