Gankyrin is essential for hypoxia enhanced metastatic potential in breast cancer cells

Gao, Liucun; Xie, Huahong; Dong, Lei; Jia, Zhenquan; Fu, Jie; Gao, Xin; Ou, Lun; Xiang, Song; Song, Haifeng

doi:10.3892/mmr.2013.1860

Cited by 26 publications

(14 citation statements)

References 20 publications

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“…According to the literature, E‐cadherin is upregulated by ISG15 as well as by CLDN3 and FERMT2 shown in Table , which were all detected in MCS cells with higher LFQs than in controls. In addition, E‐cadherin is downregulated by MAPK8 and BCAR1, which are accumulated to a higher level in AD than in MCS cells, as well as by PSMD10 and SRC, which are absent in AD but present in MCS cells (Table ) . As our measurements clearly indicated a reduction of E‐cadherin in MCS cells, it may be concluded that the proteins effecting lower amounts of E‐cadherin are dominant.…”

Section: Resultssupporting

confidence: 49%

Decreased E‐Cadherin in MCF7 Human Breast Cancer Cells Forming Multicellular Spheroids Exposed to Simulated Microgravity

et al. 2018

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MCF7 human breast cancer cells were cultured under normal gravity (1 g) and on a random positioning machine (RPM) preventing sedimentation. After 2 weeks, adherent 1 g-control and adherent RPM cells (AD) as well as multicellular spheroids (MCS) were harvested. AD and MCS had been exposed to the RPM in the same culture flask. In a subsequent proteome analysis, the majority of the proteins detected showed similar label-free quantification (LFQ) scores in each of the respective subpopulations, but in both AD or MCS cultures, proteins were also found whose LFQs deviated at least twofold from their counterparts in the 1 g-control cells. They included the cell junction protein E-cadherin, which was diminished in MCS cells, where proteins of the E-cadherin autodegradation pathway were enhanced and c-Src (proto-oncogene tyrosine-protein kinase c-Src) was detected. Spheroid formation was prevented by inhibition of c-Src but promoted by antibodies blocking E-cadherin activity. An interaction analysis of the detected proteins that are involved in forming and regulating junctions or adhesion complexes and in E-cadherin autodegradation indicated connections between the two protein groups. This suggests that the balance of proteins that up- or downregulate E-cadherin mediates the tendency of MCF7 cells to form MCS during RPM exposure.

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Section: Resultssupporting

confidence: 49%

Decreased E‐Cadherin in MCF7 Human Breast Cancer Cells Forming Multicellular Spheroids Exposed to Simulated Microgravity

et al. 2018

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“…The aberrant expression of PSMD10 has been found in colorectal cancer, breast cancer, and liver cancer [4,20–22]. Aberrant PSMD10 expression has a function in tumor metastasis, proliferation, and drug resistance [4,6,22–24]. The results of the present study suggest that PSMD10 was directly targeted by miR‐605.…”

Section: Discussionmentioning

confidence: 53%

MiR‐605 represses PSMD10/Gankyrin and inhibits intrahepatic cholangiocarcinoma cell progression

Tian

et al. 2014

FEBS Letters

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a b s t r a c tThe aberrant expression of PSMD10 has important functions in various malignancies. This study showed that PSMD10 was highly expressed and inversely correlated with the expression of miR-605 in intrahepatic cholangiocarcinoma (ICC) specimens. MiR-605 directly targeted and repressed PSMD10 expression. In addition, over-expression of miR-605 inhibited ICC cell progression both in vitro and in vivo. This effect of miR-605 on ICC cells was similar to that of PSMD10 knock-down by RNAi. Moreover, restoration of PSMD10 could reverse the phenotypic alteration caused by miR-605 in ICC cells. These results suggest a new therapeutic strategy in ICC by restoring miR-605, which is regulated by p53.

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“…In addition, gankyrin deletion abrogated the increased metastatic potential of breast cancer cells under hypoxic conditions, partly through regulating E-cadherin [27]. Gankyrin was also found to maintain the stemness of colorectal cancer and control stem cell behavior by regulating the expression of stemness factors.…”

Section: Cellular Physiology and Biochemistrymentioning

confidence: 90%

Association Between Functional PSMD10 Rs111638916 Variant Regulated by MiR-505 and Gastric Cancer Risk in a Chinese Population

Liu

Jiang

et al. 2015

Cell Physiol Biochem

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Background/Aims: Gankyrin is an oncoprotein involved in regulating the cell cycle through protein-protein interactions with cyclin-dependent kinase 4 and p53. However, its association with gastric cancer (GC) risk has not yet been determined. In this study, we investigated micro RNA (miRNA)-associated single nucleotide polymorphisms (SNPs) in the 3′-untranslated region (UTR) of the gankyrin gene PSMD10 to clarify the relationship between these SNPs and miRNAs in Chinese patients with GC. Methods: We performed a case-control study including 857 GC patients and 748 cancer-free controls. PSMD10 expression was investigated using genotyping, real-time polymerase chain reaction, cell transfection, and dual luciferase reporter assays. Results: Patients with histories of smoking, alcohol consumption, and cancer were more susceptible to GC than controls. The SNP rs111638916 in the PSMD10 3′-UTR was identified as a risk factor for GC and acted as a tumor promoting factor. SNP rs111638916 was also regulated by miR-505, resulting in up-regulation of gankyrin expression in patients with GA and AA genotypes. Carriers of the GA and AA genotypes also presented with larger tumors and had a higher risk of metastasis. Conclusion: The PSMD10 rs111638916 SNP is highly associated with an increased risk of GC in Chinese patients, and could serve as a novel biomarker for this disease.

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Gankyrin is essential for hypoxia enhanced metastatic potential in breast cancer cells

Cited by 26 publications

References 20 publications

Decreased E‐Cadherin in MCF7 Human Breast Cancer Cells Forming Multicellular Spheroids Exposed to Simulated Microgravity

Decreased E‐Cadherin in MCF7 Human Breast Cancer Cells Forming Multicellular Spheroids Exposed to Simulated Microgravity

MiR‐605 represses PSMD10/Gankyrin and inhibits intrahepatic cholangiocarcinoma cell progression

Association Between Functional PSMD10 Rs111638916 Variant Regulated by MiR-505 and Gastric Cancer Risk in a Chinese Population

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