Ganoderic acid T from Ganoderma lucidum mycelia induces mitochondria mediated apoptosis in lung cancer cells

Tang, Wen; Liu, Jianwen; Zhao, Weiming; Wei, Dongzhi; Zhong, Jian‐Jiang

doi:10.1016/j.lfs.2006.09.001

Cited by 232 publications

(145 citation statements)

References 21 publications

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“…They are a group of wood-degrading mushrooms with hard fruiting bodies comprising a pileus, spore, and stipe [1]. For over a century, Ganoderma species extracts have been traditionally used as a Chinese medicinal mushroom for the treatment of hepatitis [2,3], cancer-related fatigue and immune functions [4,5], neurasthenia [6], and cancer [7], where Ganoderma extract has been shown to have anticancer activity against MCF-7 and MDA-MB-231 breast cancer cells [8,9], 95-D lung cancer cells [10], PC-3 prostate cancer cells [9], and HUC-PC and MTC-11 bladder cancer cells [11]. To date, more than 100 ganoderic acids, a kind of highly oxygenated C30 lanostanetype triterpenoid, have been isolated from Ganoderma species [1] and they were active forms that exert various pharmacological activities, as mentioned above.…”

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Detection of Ganoderic Acid A in Ganoderma lingzhi by an Indirect Competitive Enzyme-Linked Immunosorbent Assay

et al. 2016

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Ganoderma is a genus of medicinal mushroom traditionally used for treating various diseases. Ganoderic acid A is one of the major bioactive Ganoderma triterpenoids isolated from Ganoderma species. Herein, we produced a highly specific monoclonal antibody against ganoderic acid A (MAb 12?A) and developed an indirect competitive ELISA for the highly sensitive detection of ganoderic acid A in Ganoderma lingzhi, with a limit of detection of 6.10?ng/mL. Several validation analyses support the accuracy and reliability of the developed indirect competitive ELISA for use in the quality control of Ganoderma based on ganoderic acid A content. Furthermore, quantitative analysis of ganoderic acid A in G. lingzhi revealed that the pileus exhibits the highest ganoderic acid A content compared with the stipe and spore of the fruiting body; the best extraction efficiency was found when 50?% ethanol was used, which suggests the use of a strong liquor to completely harness the potential of Ganoderma triterpenoids in daily life.

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Detection of Ganoderic Acid A in Ganoderma lingzhi by an Indirect Competitive Enzyme-Linked Immunosorbent Assay

et al. 2016

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“…The structure of the triterpenoid ganoderic acid from fungi is similar to FPOA, the primary difference being the carboxyl position. Previous studies have revealed that ganoderic acid induces apoptosis in numerous tumor cells, including Bel-7402, HepG2 and HeLa cells (23,24). Additionally, various molecular mechanisms for this effect have been reported, including apoptosis-associated proteins, death receptors, oxidative stress and immunomodulation (23,24).…”

Section: Discussionmentioning

confidence: 99%

FPOA induces the apoptosis of HepG2 cells

2018

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Abstract. The triterpenoid 3-acetoxylanosta-8,24-dien-21-oic acid (FPOA) is isolated from the fruiting body of Fomitopsis pinicola. The present study reports that FPOA exerts cytotoxic activity and describes the molecular mechanism of FPOA-induced apoptosis on human HepG2 hepatoma cells. FPOA exhibited significant cytotoxic effects against HepG2, MCF-7 and HeLa cells. However, FPOA was particularly cytotoxic towards HepG2 cells, with a half maximal inhibitory concentration value of 42.10 µM, thus these cells were taken forward for further analysis. Flow cytometry results demonstrated that FPOA significantly increased the apoptotic rate of HepG2 cells in a dose-dependent manner, explaining its potent cytotoxicity. In addition, western blot analysis revealed that FPOA significantly increased the B-cell lymphoma 2 (Bcl-2)-associated X/Bcl-2 ratio, and cytochrome c, caspase-9 and caspase-3 release, in addition to significantly decreasing poly(ADP) ribose polymerase levels. These observations indicate that FPOA induces the apoptosis of HepG2 cells by activating members of the caspase protein family and triggering the mitochondrial apoptosis signaling pathway. Based on these results, FPOA is a potential agent for the treatment of cancer.

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“…We have previously demonstrated that other triterpenes, ganoderic acid A and H, suppressed growth of breast cancer cells through the inhibition of expression of Cdk-4 (31). Moreover, ganoderic acid DM suppressed proliferation of prostate cancer cells and inhibited 5·-reductase activity (32), whereas ganoderic acid T suppressed proliferation and tumor growth in mice by the up-regulation of expression of tumor suppressor p53 and by the induction of apoptosis (33), and ganoderic acid X suppressed proliferation of hepatocellular carcinoma cells through the inhibition of topoisomerase I and II (34). In addition, lucidenic acid A, C and N induced cell cycle arrest at G1 phase, whereas lucidenic acid B induced apoptosis of leukemia cells (35).…”

Section: Discussionmentioning

confidence: 99%

Ganodermanontriol, a lanostanoid triterpene from Ganoderma lucidum, suppresses growth of colon cancer cells through ß-catenin signaling

Jedinak

Thyagarajan‐Sahu²,

Jiang³

et al. 2011

Int J Oncol

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Abstract. Colorectal cancer is one of the most common cancers in men and women in the world. Previous molecular studies have revealed that deregulation of the ß-catenin signaling pathway plays a crucial role in the progression of colorectal cancer. Therefore, modulation of the ß-catenin pathway offers a strategy to control colorectal cancer progression. The medicinal mushroom Ganoderma lucidum (GL) is a rich source of triterpenes with anticancer properties. Here, we show that ganodermanontriol (GNDT), a purified triterpene from GL, inhibited proliferation of HCT-116 and HT-29 colon cancer cells without a significant effect on cell viability. Moreover, GNDT inhibited transcriptional activity of ß-catenin and protein expression of its target gene cyclin D1 in a dose-dependent manner. A marked inhibition effect was also seen on Cdk-4 and PCNA expression, whereas expression of Cdk-2, p21 and cyclin E was not affected by the GNDT treatment. In addition, GNDT caused a dosedependent increase in protein expression of E-cadherin and ß-catenin in HT-29 cells. Finally, GNDT suppressed tumor growth in a xenograft model of human colon adenocarcinoma cells HT-29 implanted in nude mice without any sideeffects and inhibited expression of cyclin D1 in tumors. In conclusion, our data suggest that ganodermanontriol might be a potential chemotherapeutic agent for the treatment of cancer.

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Ganoderic acid T from Ganoderma lucidum mycelia induces mitochondria mediated apoptosis in lung cancer cells

Cited by 232 publications

References 21 publications

Detection of Ganoderic Acid A in Ganoderma lingzhi by an Indirect Competitive Enzyme-Linked Immunosorbent Assay

Detection of Ganoderic Acid A in Ganoderma lingzhi by an Indirect Competitive Enzyme-Linked Immunosorbent Assay

FPOA induces the apoptosis of HepG2 cells

Ganodermanontriol, a lanostanoid triterpene from Ganoderma lucidum, suppresses growth of colon cancer cells through ß-catenin signaling

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