Ganoderma lucidum polysaccharides enhance the function of immunological effector cells in immunosuppressed mice

Optimization of fermentation media composition of ganoderma lucidum for improved production of antioxidant and antimicrobial compounds

“…Results of experiment shows that modified MCM medium containing maltose and NH 4 Cl as chief carbon and nitrogen source was suitable to achieved enhanced production of exopolysaccharide (shown in table 2) and antimicrobial compound as compared to standard MCM production in previous reports. From the aforementioned results, indicated that modified MCM (medium 8) could be used for large scale production of these two compounds as it is more cost effective than MCM.…”

“…The samples demonstrate antioxidant capacity in dose dependent manner in all assays. Scavenging activity of hydroxyl ion (52.7±1.55%) was found potent in medium contained maltose and NH 4 Cl, which is above than ascorbic acid at same concentration (300 μg/mL). Chloroform: methanol extract shows inhibitory effect to all five human pathogenic strains, potent zone of inhibition was seen against Shigella dysenteriae (16 to grow into the fruiting bodies on solid substrates.…”

“…As carbon sources dextrose, lactose and maltose were tested and supplemented to the MCM in same concentrations. As nitrogen sources, peptone powder, soyapeptone, NH 4 Cl, NH 4 NO 3 , and (NH 4 ) 2 SO 4 were tested. Fifteen different medium compositions were prepared represented as medium (1 to 15) shown in table 1.…”

“…[2] It has been well documented that polysaccharides from G. lucidum are one of the major sources which possess many bioactivities including antitumor, [3] immunomodulation [4] and antioxidation. [5] In the last three decades the search for new therapeutic bioactive compounds that can serve as antioxidant and antimicrobial agents had increased tremendously due to multiple drug resistance in human pathogenic microorganisms.…”

“…The antioxidant capacity was determined by three methods: Hydrogen peroxide assay, reducing power assay and hydroxyl radical scavenging assay in vitro while potential of antibacterial compound was determined by Agar well diffusion method. Results: High productivity were seen in medium contained maltose as carbon and soyapeptone, NH 4 Cl, NH 4 NO 3 as nitrogen source for both exopolysaccharide and antimicrobial compound. The samples demonstrate antioxidant capacity in dose dependent manner in all assays.…”