Ganoderma Lucidum Protects Rat Brain Tissue Against Trauma-Induced Oxidative Stress

Özevren, Hüseyin; İrtegün, Sevgi; Deveci, Engin; Aşır, Fırat; Pektanc, Gulsum; Deveci, Senay

doi:10.13004/kjnt.2017.13.2.76

Cited by 9 publications

(6 citation statements)

References 38 publications

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“…This result indicated that GLTs could potentially exert a protective effect on AD. Similar to our results, Özevren et al provide evidence for G. lucidum protects rat brain tissue against traumainduced oxidative stress [37]. So, we concluded that GLTs relieved oxidative damage and inactivated the ROCK signaling pathway to play a protective role in AD mice and the multitarget effects of GLTs may have desirable advantages for the treatment of multifactorial neurodegenerative diseases such as AD.…”

Section: Discussionsupporting

confidence: 91%

Ganoderma lucidum Triterpenoids (GLTs) Reduce Neuronal Apoptosis via Inhibition of ROCK Signal Pathway in APP/PS1 Transgenic Alzheimer’s Disease Mice

Yu¹,

Huang

Jiang³

et al. 2020

Oxidative Medicine and Cellular Longevity

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Alzheimer’s disease (AD) is the most common cause of dementia among senior citizen. Ganoderma lucidum triterpenoids (GLTs) have nutritional health benefits and has been shown to promote health and longevity, but a protective effect of GLTs on AD damage has not yet been reported. The objective of this research was to elucidate the phylactic effect of GLTs on AD model mice and cells and to explore its underlying mechanisms. Morris water maze (MWM) test was conducted to detect changes in the cognitive function of mice. Hematoxylin-eosin (HE) staining was applied to observe pathological changes in the hippocampus. Silver nitrate staining was applied to observe the hippocampal neuronal tangles (NFTs). Apoptosis of the hippocampal neurons in mouse brain tissue was determined by TUNEL staining. The expression levels of apoptosis-related protein Bcl2, Bax, and caspase 3/cleaved caspase 3; antioxidative protein Nrf2, NQO1, and HO1; and ROCK signaling pathway-associated proteins ROCK2 and ROCK1 were measured by western blot. In vivo experiments show that 5-month-old APP/PS1 mice appeared to have impaired acquisition of spatial learning and GLTs could reduce cognitive impairment in AD mice. Compared to normal mice, the hippocampus of APP/PS1 mouse’s brains was severely damaged, while GLTs could alleviate this symptom by inhibiting apoptosis, relieving oxidative damage, and inactivating the ROCK signaling pathway. In in vitro cell experiments, Aβ25-35 was applied to induce hippocampal neurons into AD model cells. GLTs promoted cell proliferation, facilitated superoxide dismutase (SOD) expression, and inhibited malondialdehyde (MDA) and lactic dehydrogenase (LDH) expression of neurons. Our study highlights that GLTs improve cognitive impairment, alleviate neuronal damage, and inhibit apoptosis in the hippocampus tissues and cells in AD through inhibiting the ROCK signaling pathway.

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Section: Discussionsupporting

confidence: 91%

Ganoderma lucidum Triterpenoids (GLTs) Reduce Neuronal Apoptosis via Inhibition of ROCK Signal Pathway in APP/PS1 Transgenic Alzheimer’s Disease Mice

Yu¹,

Huang

Jiang³

et al. 2020

Oxidative Medicine and Cellular Longevity

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“…Cisplatin was given as a single dose of 12 mg/kg intraperitoneally according to previous studies [ 41 ]. Also, GL doses were chosen according to previous studies [ 42 – 44 ]. Also, pilot experimental trials were carried out for the same purpose.…”

Section: Methodsmentioning

confidence: 99%

“…(4) CDDP+GL daily group: rats received oral GL (500 mg/kg/day) in distilled water daily for 10 days starting 3 days before CDDP injection (12 mg/kg b.w, i.p) Cisplatin was given as a single dose of 12 mg/kg intraperitoneally according to previous studies [41]. Also, GL doses were chosen according to previous studies [42][43][44]. Also, pilot experimental trials were carried out for the same purpose.…”

Section: Assessment Of Total Phenolic Content Total Flavonoidmentioning

confidence: 99%

Ganoderma lucidum Prevents Cisplatin-Induced Nephrotoxicity through Inhibition of Epidermal Growth Factor Receptor Signaling and Autophagy-Mediated Apoptosis

Mahran

Hassan

2020

Oxidative Medicine and Cellular Longevity

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Background. Cisplatin (cis-diaminedichloroplatinum, CDDP) is a broad-spectrum antineoplastic agent. However, CDDP has been blamed for its nephrotoxicity, which is the main dose-limiting adverse effect. Ganoderma lucidum (GL), a medicinal mushroom, has antioxidant and inflammatory activities. Therefore, this study is aimed at finding out the potential nephroprotection of GL against CDDP-induced nephrotoxicity in rats and the possible molecular mechanisms including the EGFR downstream signaling, apoptosis, and autophagy. Methods. Rats were given GL (500 mg/kg) for 10 days and a single injection of CDDP (12 mg/kg, i.p). Results. Nephrotoxicity was evidenced by a significant increase in renal indices and oxidative stress markers. Additionally, CDDP showed a plethora of inflammatory and apoptotic responses as evidenced by a profound increase of HMGB-1, NF-κB, and caspase-3 expressions, whereas administration of GL significantly improved all these indices as well as the histopathological insults. Renal expression of EGFR showed a similar trend after GL administration. Furthermore, activation of autophagy protein, LC3 II, was found to be involved in GL-mediated nephroprotection correlated with the downregulation of apoptotic signaling, caspase-3 and terminal deoxynucleotidyl transferase (TDT) renal expressions. Conclusion. These results suggest that GL might have improved CDDP-induced nephrotoxicity through antioxidant, anti-inflammatory, and autophagy-mediated apoptosis mechanisms and that inhibition of EGFR signaling might be involved in nephroprotection.

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“…In degenerative gland cells after endometriosis, NF-KB expression areas were observed in blood vessels, endothelium and surrounding inflammatory cells (Figure 2). As a result of accelerating endometriosis, NF-kB suppression monocytes/macrophages enhance M2 macrophage polarization and inhibit M1 macrophage polarization, developing a pro-repair environment causes neovascularization in ectopic lesions 21,26 .…”

Section: Discussionmentioning

confidence: 99%

NF-ĸB expression in endometriosis induced rat uterine tissue

Ermiş

Deveci

Aşır

2023

J. Drug Delivery Ther.

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Objective: Our aim in this study is to examine the effects of endometriosis on uterine tissue. Materials and methods: Two groups were formed, with eight rats in each group. Sham group was subjected only saline solution for 7 days. The endometriosis (EM) model was induced with estradiol benzoate (EB). In the EM group, EB was given to rats for 7 days. At the end of the experiment, blood was taken from the animals and serum total antioxidant status (TAS) and total oxidant status (TOS) values were studied. Uterine tissues were fixed in formalin and embedded in paraffin blocks. NF-ĸB 3 immune staining were performed on uterine sections. The results were examined under the microscope. Results: Compared to sham group, TAS values were significantly decreased and TOS values were significantly increased in serum of rats belonging to EM group. In the analysis of NF-ĸB expression in uterine tissue of sham group, the expression was slight and found only in connective tissue cells and inflammatory cells. NF-ĸB expression was mainly negative. After EM induction, NF-ĸB expression was increased in degenerated epithelial cells and gland cells, inflammatory cells around the glands and blood vessel endothelial cells and lamina propria. Conclusion: We think that NF-κB signal may be a determinant in the treatment of endometriosis. Keywords: endometriosis, immunohistochemistry, NF-κB, biochemistry, rat

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Ganoderma Lucidum Protects Rat Brain Tissue Against Trauma-Induced Oxidative Stress

Cited by 9 publications

References 38 publications

Ganoderma lucidum Triterpenoids (GLTs) Reduce Neuronal Apoptosis via Inhibition of ROCK Signal Pathway in APP/PS1 Transgenic Alzheimer’s Disease Mice

Ganoderma lucidum Triterpenoids (GLTs) Reduce Neuronal Apoptosis via Inhibition of ROCK Signal Pathway in APP/PS1 Transgenic Alzheimer’s Disease Mice

Ganoderma lucidum Prevents Cisplatin-Induced Nephrotoxicity through Inhibition of Epidermal Growth Factor Receptor Signaling and Autophagy-Mediated Apoptosis

NF-ĸB expression in endometriosis induced rat uterine tissue

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