Ganoderma lucidum Rhodiola compound preparation prevent d-galactose-induced immune impairment and oxidative stress in aging rat model

Abstract

Aging is an irreversible process. This research aims to study the anti-aging effects of GRCP, a compound preparation made by Ganoderma lucidum and Rhodiola rosen, in aging rats. Rats were subcutaneously injected with 400 mg/kg of d-galactose daily, and aging could be induced after 8 weeks. The aging rats were treated with GRCP. This experiment was divided into 6 groups. Rats were randomly divided into the model group, positive control group, low-dose GRCP group (25 mg/kg body weight), medium-dose GRCP group (50 mg/kg body weight), and high-dose GRCP group (100 mg/kg body weight), healthy and normal rats were used as blank controls. After the end, the results show that the use of GRCP at a dose of 100 mg/kg is the best treatment for improving aging rats. Rats gained weight, spleen and thymus indexes, and splenocyte proliferation improved, and inflammatory cytokine levels decreased. Besides, biochemical indicators show that GRCP can improve the antioxidant enzyme activity and reduce the content of lipofuscin and TGF-β in aging rats (P < 0.05). GRCP can also inhibit the activation of the MyD88/NF-κB pathway in rat hippocampus. These results seem to suggest that GRCP can be used as a potential natural supplement or functional food to prevent aging.

Metabolomics and biochemical insights on the regulation of aging-related diabetes by a low-molecular-weight polysaccharide from green microalga Chlorella pyrenoidosa

“…The average weight of normal mouse and drug-induced mice before treatment appeared to show significant differences ( p < 0.05). This could be attributable to the effects of STZ and d -gal; furthermore, mice weight loss has been considered as one of the most common indicators associated with aging and diabetes ( Suthagar et al, 2009 , Yuan et al, 2020 ). After treatment with CPP and DMBG, mice body weight appeared to recover significantly ( p < 0.05).…”

Effects of Rosa roxburghii Tratt glycosides and quercetin on D‐galactose‐induced aging mice model

“…Wuweizi syrup was found to improve free radical metabolism disorder and reduce lipid peroxidation damage, resulting in an anti‐aging effect by increasing the levels of CAT, GSH‐Px, and SOD (Peng et al, 2020). Yuan et al demonstrated that subcutaneous D‐galactose injection decreased the levels of CAT, GSH‐Px, and SOD while increasing MDA content in aging rats, which was significantly reversed by the intervention of Ganoderma lucidum Rhodiola compound preparation (GRCP) (Yuan et al, 2020). The aging model for this study was successfully constructed based on the experimental results.…”

“…Therefore, delaying the aging process and developing novel anti‐aging drugs have become critical. Aging is characterized by physical changes and dysfunction of many biological systems caused by a variety of factors, including free radicals and chronic inflammation (Schöttker et al, 2015; Yuan et al, 2020). It has been reported that excessive oxidative stress and lipid peroxides aggravate tissue and organ function decline and enter aging.…”

“…Flavonoids, which are glycosides derived from plants' major secondary metabolites, include isoquercitrin, hyperin, quercimeritrin, and quercitrin. As a natural antioxidant, the flavonoid quercetin was found in many plants and was used as an anti‐aging agent for free radical scavenging and anti‐lipid peroxidation (Li et al, 2013; Niu et al, 2021; Yuan et al, 2020). Furthermore, glycosides and quercetin inhibited oxidative, nitrosative, and inflammatory stress through the regulation of sulfotransferases (SULTS) activity.…”

Celastrol Alleviates Autoimmune Hepatitis Through the PI3K/AKT Signaling Pathway Based on Network Pharmacology and Experiments

“…Herbal medicines harbor various bioactive ingredients acting against multiple targets rather than a single target ( Yuan et al, 2020 ). In 2008, the concept of “network pharmacology” was proposed by Hopkins and has so far evolved as an emerging field of pharmacology.…”