Ganoderma tsugae extracts inhibit colorectal cancer cell growth via G2/M cell cycle arrest

Hsu, Shih Chung; Ou, Chien Chih; Li, Jhy Wei; Chuang, Tzu Chao; Kuo, Han Pin; Liu, Jah Yao; Chen, Chin Shiang; Lin, Song; Su, Ching Hua; Kao, Ming‐Ching

doi:10.1016/j.jep.2008.09.025

Cited by 59 publications

(44 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most cancer drugs induce cancerous DNA damage, block mitosis, and arrest cells at the G 2 /M phase. 18,19 However, when apoptotic cells have lost sufficient DNA, the sub-G1 peak will appear in a DNA histogram. 20 Therefore, the apoptosis and cell death induced by the A. debilis extract may have been related to sub-G 1 arrest in HT-29 cells.…”

Section: Discussionmentioning

confidence: 99%

Aristolochia debilis Sieb. et Zucc. Induces Apoptosis and Reactive Oxygen Species in the HT-29 Human Colon Cancer Cell Line

Wang²

2013

Cancer Biotherapy and Radiopharmaceuticals

View full text Add to dashboard Cite

In this study, we investigated the potential anticancer effect and mechanisms of action of a methanol extract from Aristolochia debilis Sieb. et Zucc (A. debilis) stems on human colon cancer cells (HT-29). A. debilis inhibited proliferation of HT-29 cells in dose-dependent and time-dependent manners as detected by the 1-(4,5-dimethylthiazol-2-yl)-3,5-diphenylformazan assay. A cell cycle analysis was performed via propidium iodide staining and flow cytometry. The A. debilis extract affected cell cycle progression by inducing sub-G1 arrest. The reactive oxygen species (ROS) level was determined by dichlorofluorescin diacetate. A decrease in the mitochondrial membrane potential and inhibition of manganese superoxide dismutase in combination with the accumulation of ROS induced apoptosis. Reverse transcription-polymerase chain reaction and Western blot analyses were used to determine changes in the expression of mitochondria-dependent apoptosis markers (Bax and Bcl-2). Upregulation of Bax and corresponding downregulation of Bcl-2 expression as well as ROS production may be the critical mechanism through which A. debilis induced apoptosis in HT-29 cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Aristolochia debilis Sieb. et Zucc. Induces Apoptosis and Reactive Oxygen Species in the HT-29 Human Colon Cancer Cell Line

Wang²

2013

Cancer Biotherapy and Radiopharmaceuticals

View full text Add to dashboard Cite

show abstract

“…The extract of GT (GTE) was prepared as described previously [15]. Briefly, the powder of the GT fruiting body (5 g) was soaked in 99.9% methanol (200 mL), mixed, and shaken for 24 h on a rotating shaker.…”

Section: Methodsmentioning

confidence: 99%

“…The animal experiments were performed as described previously [15] with slight modifications. Briefly, 5 × 10 6 SKOV-3 cells were subcutaneously implanted into the flank region of female BALB/c nude mice (BALB/cAnN.Cg- Foxn1 nu /CrlNarl).…”

Section: Methodsmentioning

confidence: 99%

“…In addition, Ganoderma tsugae (GT), another well-cultivated species of Ganoderma , has been shown to have many biological and pharmacological properties, such as antiautoantibody formation [10], antifibrosis [11], antiinflammation [12], and antioxidation characteristics [13]. A number of reports show that GT has growth-inhibitory effects in a variety of human cancer cells, such as MDA-MB-231 and MCF-7 breast cancer cells [14], COLO 205 colorectal cancer cells [15], A431 epidermoid carcinoma cells [16], Hep3B hepatoma cells [17], and H23 and H23/0.3 lung adenocarcinoma cells [18]. Although GT has antitumor activity in many human cancer cells, the mechanisms that underlie its growth-inhibitory effect on HER2-overexpressing cancer cells remain unclear.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ganoderma tsugaeExtract Inhibits Growth of HER2-Overexpressing Cancer Cells via Modulation of HER2/PI3K/Akt Signaling Pathway

Kuo

Hsu

Ou³

et al. 2013

Evidence-Based Complementary and Alternative Medicine

Self Cite

View full text Add to dashboard Cite

Ganoderma, also known as Lingzhi or Reishi, has been used for medicinal purposes in Asian countries for centuries. It is a medicinal fungus with a variety of biological properties including immunomodulatory and antitumor activities. In this study, we investigated the molecular mechanisms by which Ganoderma tsugae (GT), one of the most common species of Ganoderma, inhibits the proliferation of HER2-overexpressing cancer cells. Here, we show that a quality assured extract of GT (GTE) inhibited the growth of HER2-overexpressing cancer cells in vitro and in vivo and enhanced the growth-inhibitory effect of antitumor drugs (e.g., taxol and cisplatin) in these cells. We also demonstrate that GTE induced cell cycle arrest by interfering with the HER2/PI3K/Akt signaling pathway. Furthermore, GTE curtailed the expression of the HER2 protein by modulating the transcriptional activity of the HER2 gene and the stability/degradation of the HER2 protein. In conclusion, this study suggests that GTE may be a useful adjuvant therapeutic agent in the treatment of cancer cells that highly express HER2.

show abstract

“…lucidum biometabolites flavonoids, terpenoids, phenolics and alkaloids showed anti-human papillomavirus 16 (HPV 16) E6 oncoprotein activity.Dichloromethane extract from G.lucidum inhibited the HPV 16 E6 production in epidermoid cervical carcinoma (CaSki) cells 38 . Tumorigenic in vitro and in vivo study of colorectal adenocarcinoma cells in nude mice revealed that Ganoderma tsugae extracts inhibited the proliferation of colorectal adenocarcinoma cells, possibly due to down regulation of cyclin A and B1 and up regulation of p21 and p27 that caused arrest of G2/M cell cycle 39 .…”

Section: Pleurotus Spmentioning

confidence: 99%

Untitled

2013

IJPSR

View full text Add to dashboard Cite

Ganoderma tsugae extracts inhibit colorectal cancer cell growth via G2/M cell cycle arrest

Cited by 59 publications

References 24 publications

Aristolochia debilis Sieb. et Zucc. Induces Apoptosis and Reactive Oxygen Species in the HT-29 Human Colon Cancer Cell Line

Aristolochia debilis Sieb. et Zucc. Induces Apoptosis and Reactive Oxygen Species in the HT-29 Human Colon Cancer Cell Line

Ganoderma tsugaeExtract Inhibits Growth of HER2-Overexpressing Cancer Cells via Modulation of HER2/PI3K/Akt Signaling Pathway

Untitled

Contact Info

Product

Resources

About