Gantrez® AN nanoparticles as an adjuvant for oral immunotherapy with allergens

Gómez, Sara; Gamazo, Carlos; Román, Beatriz San; Ferrer, Marta; Sánz, M L; Irache, Juan M.

doi:10.1016/j.vaccine.2007.05.020

Cited by 75 publications

(41 citation statements)

References 42 publications

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“…These nanoparticles demonstrated their ability to initiate a strong and balanced mucosal immune response and then, to efficiently induce Th-1 subset [5]. In addition, these nanoparticles loaded with different antigens have showed to be effective against experimental challenges with Salmonella or Brucella [6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Mucosal immunization with Shigella flexneri outer membrane vesicles induced protection in mice

Camacho

Souza

Sánchez-Gómez

et al. 2011

Vaccine

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Section: Introductionmentioning

confidence: 99%

Mucosal immunization with Shigella flexneri outer membrane vesicles induced protection in mice

Camacho

Souza

Sánchez-Gómez

et al. 2011

Vaccine

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“…The liposomes were then formulated in HEC based freeze-dried rods. Additionally, a mixture of HEC and Gantrez was also used to develop freeze-dried rods because Gantrez is reported to enhance both Th1 and Th2 markers [7], and may result in effective HIV-1 elimination. The freeze-dried liposome rods were investigated for moisture content which was found to be less in case of freeze-dried liposomes as compared to freeze-dried liposomes rods (Fig.…”

Section: Resultsmentioning

confidence: 99%

Development of liposome-based freeze-dried rods for vaginal vaccine delivery against HIV-1

Gupta¹,

Pattani²,

Malcolm³

et al. 2010

Journal of Controlled Release

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The aminolysis effect on thermal behaviour of PLLA sc was investigated by DSC analysis (Fig. 2). Pristine PLLA sc thermogram shows no glass transition and a complex thermal behaviour between 150 and 190°C, due to the fusion of the original crystalline phase at 168°C, followed by recrystallization at 172°C and melting of the just-formed thicker crystals at 183 C. As far as the determination of the protein absorbed onto the APLLA sc surface is concerned, increasing amounts of the conjugated PLLA-E7 were solubilised in SDS-loading buffer (50 mM Tris-HCl pH 6.8, 3% SDS, 5% b-Mercaptoethanol, 10% glycerol), heated at 95°C for 5 min and loaded on a 15% polyacrylamide gel. After the electrophoresis the protein was stained on the gel by Coomassie blue and the amount of E7 was determined by comparing the bands in the gel with standard quantity of BSA (Fig. 3). A value of 0.1 mg of E7 per mg of APLLA sc was obtained. In order to verify the immunogenic activity of PLLA-E7 system, in vivo experiment on mice is in progress. ConclusionThe E7 protein of the Human Papillomavirus 16 (HPV16) was adsorbed on 2D-micro-1D-nano-sized poly(l-lactide) lamellar single crystals grown from dilute solution. In order to increase the interaction of the protein with polymer substrate, amino groups were introduced on the lamellae surface by aminolysis reaction. The high adsorbed protein amount opens interesting perspectives in a possible use of the developed system as single-dose vaccine. References Abstract summaryThe present investigation deals with development and characterization of the liposomes-based freeze-dried rods for the vaginal delivery of gp140 antigen in mice. Positively charged, negatively charged and neutral liposomes were prepared and characterized for various parameters e.g. morphology, size, polydispersity index, zeta potential and antigen encapsulation efficiency. To further improve the efficacy of vaccine delivery, antigen encapsulated liposomes were formulated as polymer gel-based freeze-dried rods, which were then characterized for moisture content. The redispersibility of the liposomes-based freezedried rods was determined in simulated vaginal fluid and liposome gel was investigated for mucoadhesion. The developed liposome-based freeze-dried rods systems could offer potential as stable and practical dosage form for the mucosal immunization against HIV-1 infection. IntroductionThe HIV epidemic has become one of the main global health problems. Vaginal vaccination approaches are well documented in the literature; most involve the administration of antigen in simple buffer solutions rather than delivery modalities specifically designed for vaginal administration [1][2].From a formulation perspective, inducing effective antigen specific immune responses by cervicovaginal instillation of buffer solution containing solubilized antigen is far from ideal owing to the potential for leakage at the administration site, rapid enzymatic degradation of the antigen, the influence of the menstrual cycle, and inadequate exposure of a...

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“…These findings suggest that PVA-SPIONs possess an intrinsic capacity for immune-modulation and may potentially provide a form of tolerance induction by affecting DC function. There are several lines of evidence in the literature demonstrating that other types of nanoparticles may induce a degree of desirable immune-suppression: synthetic peptide dendrimers inhibited experimental allergic encephalomyelitis, allergen-loaded nanoparticles and dendrimers downregulated allergic reactions to environmental and food allergens, and a fullerene derivative polyhydroxy C60 prevented type I hypersensitivity reactions (Scholl et al 2004;Balenga et al 2006;Gomez et al 2007Gomez et al , 2008Wegmann et al 2008). Size, surface charge and coating may confer to a nanoparticle its intrinsic properties that may lead to immune-suppression.…”

Section: Discussionmentioning

confidence: 99%

Biomedical nanoparticles modulate specific CD4⁺T cell stimulation by inhibition of antigen processing in dendritic cells

et al. 2011

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Understanding how nanoparticles may affect immune responses is an essential prerequisite to developing novel clinical applications. To investigate nanoparticle-dependent outcomes on immune responses, dendritic cells (DCs) were treated with model biomedical poly(vinylalcohol)-coated super-paramagnetic iron oxide nanoparticles (PVA-SPIONs). PVA-SPIONs uptake by human monocyte-derived DCs (MDDCs) was analyzed by flow cytometry (FACS) and advanced imaging techniques. Viability, activation, function, and stimulatory capacity of MDDCs were assessed by FACS and an in vitro CD4 + T cell assay. PVA-SPION uptake was dose-dependent, decreased by lipopolysaccharide (LPS)-induced MDDC maturation at higher particle concentrations, and was inhibited by cytochalasin D pre-treatment. PVA-SPIONs did not alter surface marker expression (CD80, CD83, CD86, myeloid/plasmacytoid DC markers) or antigen-uptake, but decreased the capacity of MDDCs to process antigen, stimulate CD4 + T cells, and induce cytokines. The decreased antigen processing and CD4 + T cell stimulation capability of MDDCs following PVA-SPION treatment suggests that MDDCs may revert to a more functionally immature state following particle exposure.

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Gantrez® AN nanoparticles as an adjuvant for oral immunotherapy with allergens

Cited by 75 publications

References 42 publications

Mucosal immunization with Shigella flexneri outer membrane vesicles induced protection in mice

Mucosal immunization with Shigella flexneri outer membrane vesicles induced protection in mice

Development of liposome-based freeze-dried rods for vaginal vaccine delivery against HIV-1

Biomedical nanoparticles modulate specific CD4⁺T cell stimulation by inhibition of antigen processing in dendritic cells

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Gantrez® AN nanoparticles as an adjuvant for oral immunotherapy with allergens

Cited by 75 publications

References 42 publications

Mucosal immunization with Shigella flexneri outer membrane vesicles induced protection in mice

Mucosal immunization with Shigella flexneri outer membrane vesicles induced protection in mice

Development of liposome-based freeze-dried rods for vaginal vaccine delivery against HIV-1

Biomedical nanoparticles modulate specific CD4+T cell stimulation by inhibition of antigen processing in dendritic cells

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Product

Resources

About

Biomedical nanoparticles modulate specific CD4⁺T cell stimulation by inhibition of antigen processing in dendritic cells