Ganzzell‐ISPR‐Prozessentwicklung: Chancen und Risiken

Takors, R.

doi:10.1002/cite.200407040

Cited by 13 publications

(4 citation statements)

References 19 publications

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“…On the other hand, most in situ removed products are organic substances themselves and therefore easy to extract using an organic phase. Besides these advantages, ISPR has some disadvantages like higher investment, the coupling of reaction and separation conditions thus limiting individual optimization, limited knowledge on scaleup, and a lack of experience [14]. To seriously consider ISPR in the process design for new production systems, a systematic collection of data is needed.…”

Section: Introductionmentioning

confidence: 99%

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Process Design Aspects for Reaction‐Integrated Adsorption in Multi‐Enzymatic Catalysis*

Waluga

Scholl

2015

Chem Eng & Technol

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This work deals with aspects of process design for the in situ product removal via adsorption in a tri‐enzymatic synthesis of laminaribiose. Regarding the reaction, mass transfer properties of the reactants in chitosan as a potential immobilization matrix are discussed. For all three enzymes, three significantly different Thiele moduli were calculated. Also, the laminaribiose‐specific properties of mass transfer into two different adsorbents are presented. While the diffusion coefficient for adsorption in a powder zeolite is smaller than the apparent coefficient in an extrudate, the total adsorption time in extrudates is significantly longer. Experimental single‐component adsorption equilibrium data for all reactants and products are presented. Simulation is used to discuss the influence and impact of co‐adsorption and the amount of enzyme used on the yield for continuous in situ adsorption. No significant effects were observed at high residence times of adsorbent within the reaction. However, the yield can be increased significantly at short residence times of the adsorbent, depending on the adsorption properties.

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Section: Introductionmentioning

confidence: 99%

“…To seriously consider ISPR in the process design for new production systems, a systematic collection of data is needed. It is a current research topic to evaluate biotechnological processes in order to identify and estimate the potential of ISPR in white biotechnology [14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Process Design Aspects for Reaction‐Integrated Adsorption in Multi‐Enzymatic Catalysis*

Waluga

Scholl

2015

Chem Eng & Technol

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“…Adequately addressing all these points may lead to prolonged process development times and an extended time-to-market for a new product. In consequence, a less elaborated but faster process may be preferred [91]. As one of the few publications on industrial-scale isoprenoid bioprocesses the biological production of isoprene illustrates the aforementioned challenges [105].…”

Section: Challenges Of Ispr Implementationmentioning

confidence: 99%

Bioprocess Engineering for Microbial Synthesis and Conversion of Isoprenoids

Schewe

Mirata²,

Schrader

2015

Biotechnology of Isoprenoids

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Isoprenoids represent a natural product class essential to living organisms. Moreover, industrially relevant isoprenoid molecules cover a wide range of products such as pharmaceuticals, flavors and fragrances, or even biofuels. Their often complex structure makes chemical synthesis a difficult and expensive task and extraction from natural sources is typically low yielding. This has led to intense research for biotechnological production of isoprenoids by microbial de novo synthesis or biotransformation. Here, metabolic engineering, including synthetic biology approaches, is the key technology to develop efficient production strains in the first place. Bioprocess engineering, particularly in situ product removal (ISPR), is the second essential technology for the development of industrial-scale bioprocesses. A number of elaborate bioreactor and ISPR designs have been published to target the problems of isoprenoid synthesis and conversion, such as toxicity and product inhibition. However, despite the many exciting applications of isoprenoids, research on isoprenoid-specific bioprocesses has mostly been, and still is, limited to small-scale proof-of-concept approaches. This review presents and categorizes different ISPR solutions for biotechnological isoprenoid production and also addresses the main challenges en route towards industrial application.

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“…There are several publications in which the use of in situ magnetic separation is described for either the purification of peptides and enzymes [13 -15] or cells [16,17]. Furthermore, the in situ product removal from whole cell fermentations in general has been published also in reviews, in which the technology is discussed from the industrial point of view [18,19]. The general procedure for high-gradient magnetic fishing (HGMF) is as follows.…”

Section: Introductionmentioning

confidence: 99%

Novel In Situ Magnetic Separator for Magnetic Particle‐Based Enzyme Purification or Biocatalysis

Shaikh

Seibert

Kampeis

2018

Chemie Ingenieur Technik

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A novel in situ magnetic separator is presented in which the separation unit is directly mounted on the bioreactor. Therefore, it is possible to collect the magnetic particles directly from the reaction liquid and transfer them in one step sterile out of the reactor. By consecutive flushing, the particles are concentrated and separated from the rest. The operating parameters were determined and evaluated experimentally and by computational fluid dynamics. The separator was tested in combination with a 5‐L bioreactor with glass flask. Hereby, the accumulation kinetics of the particles were analyzed and used for a simple theoretical model.

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Ganzzell‐ISPR‐Prozessentwicklung: Chancen und Risiken

Cited by 13 publications

References 19 publications

Process Design Aspects for Reaction‐Integrated Adsorption in Multi‐Enzymatic Catalysis*

Process Design Aspects for Reaction‐Integrated Adsorption in Multi‐Enzymatic Catalysis*

Bioprocess Engineering for Microbial Synthesis and Conversion of Isoprenoids

Novel In Situ Magnetic Separator for Magnetic Particle‐Based Enzyme Purification or Biocatalysis

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