“…Second, the width of the welding seam is typically a few tens of micrometers, keeping the welded lines as close as possible in a micrometer scale to the microfluidic channel, thus extending the useful space of the substrate [16]. Third, it has higher bonding strength than other bonding methods [16,18,20,21]. Although there is a lack of standardization and evaluation protocol of welding strength measurements thus far, a number of prior studies have already proven that femtosecond laser direct welding shows superior bonding strength compared with conventional bonding methods (e.g., shear stress measurement, three-point bending test, fracture strength, and simple leak check) [19,21,22,23,24].…”