“Gap guidance” of fibroblasts and epithelial cells by discontinuous edged surfaces

Hamilton, David A.; Brunette, D. M.

doi:10.1016/j.yexcr.2005.06.015

Cited by 38 publications

(35 citation statements)

References 27 publications

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“…The probability of the success of a cell protrusion may depend upon stabilization and maturity of focal adhesions. This is further suggested by previous studies of cell response to discontinuous edged topographies, that demonstrated focal adhesion stability precedes cytoskeletal and morphological alignment [Hamilton and Brunette, 2005]. Furthermore, [Hamilton et al, 2005], observed that chondrocytes can migrate rapidly without overt morphological or cytoskeletal rearrangement [Hamilton et al, 2005b].…”

Section: Discussionsupporting

confidence: 56%

“…This is further suggested by previous studies of cell response to discontinuous edged topographies, that demonstrated focal adhesion stability precedes cytoskeletal and morphological alignment [Hamilton and Brunette, 2005]. Furthermore, [Hamilton et al, 2005], observed that chondrocytes can migrate rapidly without overt morphological or cytoskeletal rearrangement [Hamilton et al, 2005b]. Recently, it has been shown that paxillin is a molecular switch that can determine whether a focal adhesion will persist [ZaidelBar et al, 2007], and ultimately be stabilized by the cytoskeletal components.…”

Section: Discussionmentioning

confidence: 76%

“…Preparation for SEM analysis was performed as previously described [Hamilton and Brunette, 2005;. The samples were viewed using a Cambridge 260 stereo scanning electron microscope at 4 kV accelerating voltage.…”

Section: Scanning Electron Microscopymentioning

confidence: 99%

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Directional change produced by perpendicularly‐oriented microgrooves is microtubule‐dependent for fibroblasts and epithelium

Hamilton

Oakley

Jaeger

et al. 2009

Cell Motil. Cytoskeleton

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Anisotropic substrata such as micromachined grooves can control cell shape, orientation, and the direction of cell movement, a phenomena termed topographic guidance. Although many types of cells exhibit topographic guidance, little is known regarding cell responses to conflicting topographic cues. We employed a substratum with intersecting grooves in order to present fibroblasts and epithelial cells with conflicting topographic cues. Using time-lapse and confocal microscopy, we examined cell behavior at groove intersections. Migrating fibroblasts and epithelial cells typically extended a cell process into the intersection ahead of the cell body. After travelling along the "X" groove to enter the intersection, the leading lamellipodia of the cell body encountered the perpendicular "Y" groove, and spread latterly along the "Y" groove. The formation of lateral lamellipodia resulted in cells forming "T" or "L" morphologies, which were characterized by the formation of phosphotyrosine-rich focal adhesions at the leading edges. The "Y" groove did not prove an absolute barrier to cell migration, particularly for epithelial cells. Analysis of cytoskeletal distribution revealed that F-actin bundles did not adapt closely to the groove patterns, but typically did align to either the "X" or "Y" grooves. In contrast microtubules (MT) adapted closely to the walls. Inhibition of microtubule nucleation attenuated fibroblast and epithelial cell orientation within the intersection of the perpendicular grooves. We conclude that MT may be the prime determinant of fibroblast and epithelial cell conformation to conflicting topographies.

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Section: Discussionsupporting

confidence: 56%

Section: Discussionmentioning

confidence: 76%

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Directional change produced by perpendicularly‐oriented microgrooves is microtubule‐dependent for fibroblasts and epithelium

Hamilton

Oakley

Jaeger

et al. 2009

Cell Motil. Cytoskeleton

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“…Their applications include optical gratings, 1 photonic crystals, 2 biosensors, [3][4][5] and fabrication of surface topographies for microbiological studies. [6][7][8][9][10] There are several ways to create nanoscale pattern, but most of them, e.g., electron beam lithography (EBL) and focused ion beam lithography (FIB), have low fabrication speed and typically require complex instrumentation. Laser interference lithography (LIL) is a simple and relatively inexpensive technique to create periodic structures over large areas.…”

Section: Introductionmentioning

confidence: 99%

Large area periodic, systematically changing, multishape nanostructures by laser interference lithography and cell response to these topographies

et al. 2013

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Hamilton, Douglas W.; and Mittler, Silvia, "Large area periodic, systematically changing, multishape nanostructures by laser interference lithography and cell response to these topographies" (2013 Abstract. The fabrication details to form large area systematically changing multishape nanoscale structures on a chip by laser interference lithography (LIL) are described. The feasibility of fabricating different geometries including dots, ellipses, holes, and elliptical holes in both x-and y-directions on a single substrate is shown by implementing a Lloyd's interferometer. The fabricated structures at different substrate positions with respect to exposure time, exposure angle and associated light intensity profile are analyzed. Experimental details related to the fabrication of symmetric and biaxial periodic nanostructures on photoresist, silicon surfaces, and ion milled glass substrates are presented. Primary rat calvarial osteoblasts were grown on ion-milled glass substrates with nanotopography with a periodicity of 1200 nm. Fluorescent microscopy revealed that cells formed adhesions sites coincident with the nanotopography after 24 h of growth on the substrates. The results suggest that laser LIL is an easy and inexpensive method to fabricate systematically changing nanostructures for cell adhesion studies. The effect of the different periodicities and transition structures can be studied on a single substrate to reduce the number of samples significantly.

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“…8,15 Another approach for surface modification is microfabrication that creates geometrically defined microstructures typically used for the study of single cells in the laboratory. [15][16][17] However, some of the techniques stated previously can alter surface charge, surface energy, or surface chemistry. 11 Massaro et al, for example, have found in the case of commercially manufactured titanium dental implants a variety of elements and chemical compounds not related to the metal composition.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of TiO₂‐coated epoxy replicas with identical dual‐type surface topographies used in cell culture assays

Schüler

Künzler

Wild

et al. 2008

J Biomedical Materials Res

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The goal of this study was to reproducibly generate samples with complex surface topographies and chemistries identical to a "master surface" and to test their response in cell culture using rat calvarial cells. Negative replicas of dual-type topography were fabricated using dental impression material with half of the surface exhibiting smooth and rough topography, respectively. Positive epoxy resin replicas were cast from the same negative replica eight times consecutively and coated with a 60-nm thin film of titanium dioxide using a vapor deposition technique. Atomic force microscopy, scanning electron microscopy, confocal white light microscopy, and X-ray photoelectron spectroscopy indicated that TiO(2)-coated epoxy replicas had surface topographical features and surface compositions nearly indistinguishable from the original titanium master surfaces. The described technique showed high reproducibility over at least eight generations of replication using the same negative replica. Rat calvarial osteoblasts proliferated just as well on dual topography surfaces as on single topography surfaces. The advantage of the dual-type substrates is that they facilitate comparison within a single culture dish, thus eliminating dish-to-dish variation as well as saving material, time and costs compared to the usual method of evaluating surfaces in separate dishes.

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“Gap guidance” of fibroblasts and epithelial cells by discontinuous edged surfaces

Cited by 38 publications

References 27 publications

Directional change produced by perpendicularly‐oriented microgrooves is microtubule‐dependent for fibroblasts and epithelium

Directional change produced by perpendicularly‐oriented microgrooves is microtubule‐dependent for fibroblasts and epithelium

Large area periodic, systematically changing, multishape nanostructures by laser interference lithography and cell response to these topographies

Fabrication of TiO₂‐coated epoxy replicas with identical dual‐type surface topographies used in cell culture assays

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“Gap guidance” of fibroblasts and epithelial cells by discontinuous edged surfaces

Cited by 38 publications

References 27 publications

Directional change produced by perpendicularly‐oriented microgrooves is microtubule‐dependent for fibroblasts and epithelium

Directional change produced by perpendicularly‐oriented microgrooves is microtubule‐dependent for fibroblasts and epithelium

Large area periodic, systematically changing, multishape nanostructures by laser interference lithography and cell response to these topographies

Fabrication of TiO2‐coated epoxy replicas with identical dual‐type surface topographies used in cell culture assays

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Product

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Fabrication of TiO₂‐coated epoxy replicas with identical dual‐type surface topographies used in cell culture assays