Gap Junction-Mediated Coupling in the Postnatal Anterior Subventricular Zone

Menezes, João R.L.; Fróes, Maira Monteiro; Moura‐Neto, Vivaldo; Lent, Roberto

doi:10.1159/000017425

Cited by 24 publications

(25 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These junctions have been detected in the SVZ/RMS by electron microscopy and imunohistochemistry (Yamamoto et al, 1990;Miragall et al, 1997). We have shown previously, in acute in situ experiments, that GJ-mediated dye coupling in the SVZ/RMS presents a bistratified spatial distribution suggesting distinct roles in the physiology of this pathway (Menezes et al, 2000).…”

Section: Introductionmentioning

confidence: 83%

Gap junctions are involved in cell migration in the early postnatal subventricular zone

Marins

Xavier

Viana

et al. 2009

Developmental Neurobiology

Self Cite

View full text Add to dashboard Cite

The massive migration of neuroblasts and young neurons through the anterior extension of the postnatal subventricular zone (SVZ), known as the rostral migratory stream (RMS) is still poorly understood on its molecular basis. In this work, we investigated the involvement of gap junctional communication (GJC) in the robust centrifugal migration from SVZ/RMS explants obtained from early postnatal (P4) rats. Cells were dye-coupled in homocellular and heterocellular pairings and expressed at least two connexins, Cx 43 and 45. Treatment with the uncoupler agent carbenoxolone (CBX, 10-100 microM) reversibly reduced outgrowth from SVZ explants, while its inactive analog, glycyrhizinic acid (GZA), had no effect. Consistent with a direct effect on cell migration, time-lapse video microscopy show that different pharmacological uncouplers cause an abrupt and reversible arrest of cell movement in explants. Our results indicate that GJC is positively involved in the migration of neuroblasts within the SVZ/RMS.

show abstract

Section: Introductionmentioning

confidence: 83%

Gap junctions are involved in cell migration in the early postnatal subventricular zone

Marins

Xavier

Viana

et al. 2009

Developmental Neurobiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Electron microscopy clearly showed that glial processes progressively assemble to form thicker septa, which eventually join septa of mature parenchyma (mainly axons) to ensheath chains during the third to fourth postnatal week. Immunoreactivity for Cx43, a protein heavily expressed in astrocytic gap junctions (Yamamoto et al, 1990;Theis et al, 2003), although present in the CNS since early stages (see also Nadarajah et al, 1997;Menezes et al, 2000), was extensively detected in sharp coincidence with astrocytic tube assembly. The massive appearance of Cx-43 astrocytic gap junctions in the SVZ around P21 supports the idea of a further stabilization of the glial compartment in the perspective of isolating the neurogenetic area from mature parenchyma.…”

Section: Subsequent Postnatal Period: Assembly Of Glial Tubes and Chamentioning

confidence: 97%

Chain formation and glial tube assembly in the shift from neonatal to adult subventricular zone of the rodent forebrain

Peretto

Giachino

Aimar

et al. 2005

J of Comparative Neurology

143

195

View full text Add to dashboard Cite

The subventricular zone (SVZ) is regarded as an embryonic germinal layer persisting at the end of cerebral cortex neurogenesis and capable of generating neuronal precursors throughout life. The two distinct compartments of the adult rodent forebrain SVZ, astrocytic glial tubes and chains of migrating cells, are not distinguishable in the embryonic and early postnatal counterpart. In this study we analyzed the SVZ of mice and rats around birth and throughout different postnatal stages, describing molecular and morphological changes which lead to the typical structural arrangement of adult SVZ. In both species studied, most changes occurred during the first month of life, the transition being slightly delayed in mice, in spite of their earlier development. Important modifications affected the glial cells, eventually leading to glial tube assembly. These changes involved an overall reorganization of glial processes and their mutual relationships, as well as gliogenesis occurring within the SVZ which gives rise to glial cell subpopulations. The neuroblast cell population remained qualitatively quite homogeneous throughout all the stages investigated, changes being restricted to the relationships among cells and consequent formation of chains at about the third postnatal week. Electron microscopy showed that chain formation is not directly linked to glial tube assembly, generally preceding the occurrence of complete glial ensheathment. Moreover, chain and glial tube formation is asymmetric in the medial/lateral aspect of the SVZ, being inversely related. The attainment of an adult SVZ compartmentalization, on the other hand, seems linked to the pattern of expression of adhesion and extracellular matrix molecules.

show abstract

“…12 In vivo, CBX was administered intraperitoneally twice after intrauterine hypoxia-ischemia (HI), a loading dose (75 mg/kg; Ϸ150 mol/L) immediately after recovery of respiration, and a second (30 mg/kg) 12 hours later. Similar in vivo dosing regimens desynchronize spinal motor neuron firing 13 and directly block dye coupling of anterior subventricular cells 14 and retinal neurons. 15 …”

Section: Carbenoxolone Applicationmentioning

confidence: 99%

Blockade of Gap Junctions In Vivo Provides Neuroprotection After Perinatal Global Ischemia

Pina-Benabou

Szostak

Kyrozis

et al. 2005

Stroke

123

101

View full text Add to dashboard Cite

Background and Purpose-We investigated the contribution of gap junctions to brain damage and delayed neuronal death produced by oxygen-glucose deprivation (OGD). Methods-Histopathology, molecular biology, and electrophysiological and fluorescence cell death assays in slice cultures after OGD and in developing rats after intrauterine hypoxia-ischemia (HI). Results-OGD persistently increased gap junction coupling and strongly activated the apoptosis marker caspase-3 in slice cultures. The gap junction blocker carbenoxolone applied to hippocampal slice cultures before, during, or 60 minutes after OGD markedly reduced delayed neuronal death. Administration of carbenoxolone to ischemic pups immediately after intrauterine HI prevented caspase-3 activation and dramatically reduced long-term neuronal damage. Conclusions-Gap

show abstract

Gap Junction-Mediated Coupling in the Postnatal Anterior Subventricular Zone

Cited by 24 publications

References 44 publications

Gap junctions are involved in cell migration in the early postnatal subventricular zone

Gap junctions are involved in cell migration in the early postnatal subventricular zone

Chain formation and glial tube assembly in the shift from neonatal to adult subventricular zone of the rodent forebrain

Blockade of Gap Junctions In Vivo Provides Neuroprotection After Perinatal Global Ischemia

Contact Info

Product

Resources

About