“…Samples were obtained from broth (0.5 ml from each infection model), through the injection port, at 0.5, 1, 2, 4, 8, 24, 32, 48, and 72 h for determination of the antibiotic concentrations based on a pharmacodynamic model (SEVM or SBM). Samples were also taken from the homogenized SEVs obtained at 0, 8,24,32,48, and 72 h for determination of the antibiotic concentration within the vegetation. Samples were stored at Ϫ70°C until analysis.…”