Gas Chromatographic Detection of Diacetyl in Orange Juice

Abstract

A GLC method was developed for detection of diacetyl(2,3-butanedione) and acetoin (3-hydroxy-2-butanone) in orange juice. Retention times for diacetyl and acetoin were 2.8 + 0.03 and 6.8 + 0.02 min, respectively. Concentration by distillation was essential to detect small quantities by GLC of diacetyl but was ineffective for acetoin. A linear response of diacetyl concentration to peak area was observed between 0.05 and 0.5 ppm. The peak areas at 0.05 and 0.5 ppm diacetyl were 1242 t 203 and 20142 ? 1120, respectively. Elevating the column temperature and decreasing the carrier gas Jlow rate, increased the limit of detection for acetoin from 2100 ppm to a10 ppm, but diminished the resolution of the diacetyl peak from juice volatiles.

A Fluorescent Probe for Diacetyl Detection

“…Diacetyl reacts comparatively faster than acetoin, and based on this property, diacetyl was determined successfully in presence of acetoin [ 8 , 10 , 11 ]. Later, when GC was introduced, it became the most frequently used method for diacetyl determination [ 12 – 14 ], and sometimes HPLC is also used [ 15 ]. By virtue of high sensitivity and the strong spatial resolution of fluorescence measurements, much interest has been shown in pre-column fluorescent derivatization methods in chromatography [ 16 ].…”

D-Erythrulose Reductase Can Also Reduce Diacetyl: Further Purification and Characterization of D-Erythrulose Reductase from Chicken Liver

“…This shows that D-erythrulose reductase also plays an important role in the liver. Dicarbonyl compounds such as diacetyl, a good substrate of the enzyme, are present in many everyday foods and beverages, such as alcoholic drinks (9, JO), milk (11), butter (12), buttermilk (13), cheese (14,15), and orange juice (16). Borders and Riordan reported that about 1 mM diacetyl modified arginine residues in proteins (17), which suggests that a high concentration of diacetyl may be toxic for animals.…”

Effect of Working Atmospheres on the Detection of Diacetyl by Resistive SnO2 Sensor

“…Due to their widespread use in a variety of applications, in the recent years timeconsuming, complex, and expensive traditional techniques have been substituted by these types of sensors. It has been especially evident in the field of volatile organic compound (VOC) detection, where traditional techniques, such as chromatography-mass spectrometry [18][19][20][21], generally lack ease of use, need specialized personnel and elaborate protocols, and are characterized by high cost of the devices and insufficient flexibility. When early warning and quality monitoring applications are requested, resistive sensors are valuable alternatives.…”