Gas Chromatographic Determination of Benalaxyl Residues in Different Crops and Water

Crisippi, Teodoro; Zini, Guido; Fabbrini, R.

doi:10.1093/jaoac/76.3.650

Cited by 8 publications

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“…The activity of BX is mainly attributed to the (−)‐R‐enantiomer 11. A number of methods have been reported for the determination of BX residues in various samples, such as enzyme‐linked immunosorbent assay in water and wine12, 13 and gas chromatography in vegetables and fruits 14. The stereoselective degradation of BX in rabbit plasma,11 soils, and cucumber plants15 were investigated by chiral high‐performance liquid chromatography.…”

Section: Introductionmentioning

confidence: 99%

Stereoselective metabolism of benalaxyl in liver microsomes from rat and rabbit

et al. 2011

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Benalaxyl (BX), methyl-N-phenylacetyl-N-2,6-xylyl alaninate, is a potent acylanilide fungicide and consist of a pair of enantiomers. The stereoselective metabolism of BX was investigated in rat and rabbit microsomes in vitro. The degradation kinetics and the enantiomer fraction (EF) were determined using normal high-performance liquid chromatography with diode array detection and a cellulose-tris-(3,5-dimethylphenylcarbamate)-based chiral stationary phase (CDMPC-CSP). The t(1/2) of (-)-R-BX and (+)-S-BX in rat liver microsomes were 22.35 and 10.66 min of rac-BX and 5.42 and 4.03 of BX enantiomers. However, the t(1/2) of (-)-R-BX and (+)-S-BX in rabbit liver microsomes were 11.75 and 15.26 min of rac-BX and 5.66 and 9.63 of BX enantiomers. The consequence was consistent with the stereoselective toxicokinetics of BX in vitro. There was no chiral inversion from the (-)-R-BX to (+)-S-BX or inversion from (+)-S-BX to (-)-R-BX in both rabbit and rat microsomes. These results suggested metabolism of BX enantiomers was stereoselective in rat and rabbit liver microsomes.

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Section: Introductionmentioning

confidence: 99%

Stereoselective metabolism of benalaxyl in liver microsomes from rat and rabbit

et al. 2011

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“…Instrumental methods usually employed for benalaxyl analysissmainly RP-HPLC (Cabras et al, 1987) or GC-NPD (Crisippi et al, 1993)swork well but are timeconsuming and involve laborious extraction and cleanup procedures. Competitive enzyme-linked immunosorbent assays (ELISAs) are becoming either alternative or complementary analytical tools to conventional methods because of their desirable analytical features such as rapidity, sensitivity, selectivity, and low cost.…”

Section: Introductionmentioning

confidence: 99%

Application of an ELISA to the Determination of Benalaxyl in Red Wines

Rosso

Giraudi

Gamberini

et al. 1999

J. Agric. Food Chem.

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The applicability of an ELISA for detection and quantification of benalaxyl in red wine samples is described. The study of the influence of this matrix on the reliability of the assay indicates that red wine samples require a rapid and simple cleanup step before ELISA assay. Recovery and precision of the method were evaluated by spiking red wine samples with benalaxyl in the 0.5-24 ng/mL range. Benalaxyl can be determined with good accuracy and precision up to 0. 5 ng/mL in starting red wine samples (detection limit of 0.13 ng/mL). No false negative or positive results were obtained. Authentic red wine samples were analyzed by ELISA and by RP-HPLC. The amounts of benalaxyl found by ELISA were in good agreement with RP-HPLC analysis.

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Stereoselective determination of benalaxyl in plasma by chiral high‐performance liquid chromatography with diode array detector and application to pharmacokinetic study in rabbits

et al. 2006

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A chiral high-performance liquid chromatography method with diode array detector was developed and validated for stereoselective determination of benalaxyl (BX) in rabbit plasma. Good separation was achieved at 20 degrees C using cellulose tris-(3,5-dimethylphenylcarbamate) as chiral stationary phase, a mixture of n-hexane and 2-propanol (97:3) as mobile phase at a flow rate of 1.0 ml/min. The assay method was linear over a range of concentrations (0.25-25 microg/ml) in plasma and the mean recovery was greater than 90% for both enantiomers. The limits of quantification and detection for both enantiomers in plasma were 0.25 and 0.1 microg/ml, respectively. Intra- and interday relative standard deviations (RSDs) did not exceed 10% for three-tested concentrations. The method was successfully applied to pharmacokinetic studies of BX enantiomers in rabbits. The result suggested that the pharmacokinetics of BX enantiomers was stereoselective in rabbits.

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Gas Chromatographic Determination of Benalaxyl Residues in Different Crops and Water

Cited by 8 publications

References 0 publications

Stereoselective metabolism of benalaxyl in liver microsomes from rat and rabbit

Stereoselective metabolism of benalaxyl in liver microsomes from rat and rabbit

Application of an ELISA to the Determination of Benalaxyl in Red Wines

Stereoselective determination of benalaxyl in plasma by chiral high‐performance liquid chromatography with diode array detector and application to pharmacokinetic study in rabbits

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