Gas chromatographic determination of free and solvalysable dehydroepiandrosterone and androsterone in blood

Fehér, T; Bodrogi, L.; Fehér, Katalin G.; Poteczin, E

doi:10.1007/bf02274465

Cited by 5 publications

(6 citation statements)

References 13 publications

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“…This concentration exceeds the physiological serum level of DEA with about one order of magnitude [20]. Recently, how ever, it was demonstrated [21] that DEA is present in various tissues at a concentration of 1 pg/ml or more.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of the Complement Activation by an Adrenal Androgen, Dehydroepiandrosterone

Hidvegi

Fehér

et al. 1984

Complement

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The effect of dehydroepiandrosterone (DEA), an adrenal androgen successfully used for preventing attacks in hereditary angioneurotic edema (HANE) patients was studied on the activation of classical and alternative complement pathway. The steroid inhibited both the spontaneous and immune activation of the classical complement pathway (CP), the former effect, however, was found to be more marked than the latter one. DEA exerted its inhibiting effect most probably by interfering with the internal activation of C1. Because DEA rendered HANE patients symptom free but induced only a slight increase in their serum C1 - INH level, our present findings suggest that inhibition of CP activation may have a significance in the therapeutic effect of DEA and possibly of other androgens as well.

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Section: Discussionmentioning

confidence: 99%

Inhibition of the Complement Activation by an Adrenal Androgen, Dehydroepiandrosterone

Hidvegi

Fehér

et al. 1984

Complement

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“…The extracted steroid represents both protein-bound and unbound fractions since organic solvents denature the binding proteins. Preferred solvents for extracting nonpolar, hydrophobic steroids (e.g., androgens, oestrogens) are diethylether or benzene (Cook et al, 1982;Feher et al, 1977;Kling and diZerega, 1982;Moneti et al, 1987) whereas more polar solvents (dichloromethane, chloroform, ethylacetate) are useful for a broader range of steroids including corticosteroids (Allenmark ei al., 1981;Barron et al, 1979;HalperinWalega and Greene, 1985;Wiebe et al, 1980). The recovery of polar steroids and steroid conjugates is improved by addition of inorganic salts (e.g., sodium chloride, ammonium sulphate) to samples (Barron et al, 1979;Leuvering et al, 1983).…”

Section: Extractionmentioning

confidence: 98%

“…Steroid recovery during these manipulations can be assessed by the addition of 14C ( h r d y et al, 1980), deuterium (Reiffsteck et al, 1982) or tritium labelled steroids to samples (Kuelpmann and Breuer, 1977;Feher et al, 1977;Fotsis, 1987;Fotsis and Adlercreutz, 1987;Moneti et al, 1987). The addition of unlabelled steroids (Cook et al, 1982;Darney et al, 1983;Halperin-Walega and Greene, 1985;Numazawa et al, 1985;Shimada et al, 1982b;Shinohara et al, 1980) and n-alkanes (Barron et al, 1979) although less precise has also been used.…”

Section: Recovery Proceduresmentioning

confidence: 99%

“…Despite pioneering work on steroid determination in saliva in 1959 (Shannon et al, 1959), clinical chemists ignored saliva as a useful sample until the late 1970s (Vining and McGinley, 1987). Serum and plasma (heparin or EDTA) (Feher et al, 1977) are both appropriate for clinical investigations where rapid and repeated analyses are required. Erythrocytes should be separated rapidly by centrifugation in order to minimize steroid metabolism following sampling of blood.…”

Section: Samples Sampling and Storagementioning

confidence: 99%

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Chromatography as a reference technique for the determination of clinically important steroids

Robards

Towers

1990

Biomedical Chromatography

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Chromatographic methods (paper chromatography, thin layer chromatography, high performance liquid chromatography, gas chromatography and gas chromatography-mass spectrometry) for the determination of clinically important steroids in biological specimens are reviewed. The emphasis is on the use of gas chromatography, gas chromatography-mass spectrometry and high performance liquid chromatography as reference rather than routine techniques. Chromatographic methods are compared with colorimetric, fluorimetric and radioimmunoassay procedures in terms of simplicity of operation, cost and ability to analyse large numbers of specimens. The importance of correct specimen collection and storage are discussed. Sample preparation techniques for the various analytical methods are described. These include extraction of free and conjugated steroids from serum, plasma, urine and saliva by solvent partition, with polymer-based resins such as Amberlite XAD-2, DEAE-Sephadex and Sephadex resins bonded with various other function groups and, more recently, with chemically bonded reversed-phase silicas.

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“…The gonads, adrenals and thymuses were weighed. The heart blood was pooled two by two and the androsterone in the serum was determined by gas chromatography (10). The data were analyzed by Student's t test and analysis of variance, wherever possible.…”

Section: Methodsmentioning

confidence: 99%

Long-Lasting Effect of Single Ecdysone Injections in Newborn Rats

Csaba¹,

Darvas²,

László³

et al. 1978

Neonatology

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Gas chromatographic determination of free and solvalysable dehydroepiandrosterone and androsterone in blood

Cited by 5 publications

References 13 publications

Inhibition of the Complement Activation by an Adrenal Androgen, Dehydroepiandrosterone

Inhibition of the Complement Activation by an Adrenal Androgen, Dehydroepiandrosterone

Chromatography as a reference technique for the determination of clinically important steroids

Long-Lasting Effect of Single Ecdysone Injections in Newborn Rats

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