Gas chromatographic determination of poly-β-hydroxybutyric acid in microbial biomass after hydrochloric acid propanolysis

Riis, V.; Mai, Weimei

doi:10.1016/s0021-9673(01)84535-0

Cited by 323 publications

(158 citation statements)

References 4 publications

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“…The correlation factor was calculated from slope of the plot and 1 g L -1 CDM correlated to 2.476 OD. PHB content inside the cells was analyzed by gas chromatography (GC 2010 Shimadzu Co., Japan), and benzoic acid was used as an internal standard 23 . Residual sucrose concentration was determined by the Di-Nitro Salicylic acid (DNS) method 24 .…”

Section: Methodsmentioning

confidence: 99%

Enhancing the Production of Polyhydroxyalkanoate Biopolymer by Azohydromonas Australica Using a Simple Empty and Fill Bioreactor Cultivation Strategy

Gahlawat¹,

Srivastava²

2018

Chem.Biochem.Eng.Q.

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Polyhydroxyalkanoates (PHAs) are biodegradable polymers which are considered as an effective alternative for conventional plastics due to their mechanical properties similar to the latter. However, widespread use of these polymers is still hampered due to their high cost of production. This shortcoming could partly be resolved by obtaining high yields and productivity. In the present study, a drain-and-fill strategy of repeated-batch cultivation was adopted for the enhanced production of polyhydroxybutyrate PHB using Azohydromonas australica. In this strategy, 20 % (v/v) of the culture broth was removed from the reactor and supplemented with an equal volume of fresh medium. This strategy demonstrated a 3.3 fold and 1.8 fold increase in PHB concentration and productivity, respectively, as compared to batch cultivation. Repeated cultivation had also the benefit of avoiding non-productive time required for cleaning, refilling and sterilization of bioreactor during batch, thereby increasing the overall volumetric productivity and industrial importance of the process.

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Section: Methodsmentioning

confidence: 99%

Enhancing the Production of Polyhydroxyalkanoate Biopolymer by Azohydromonas Australica Using a Simple Empty and Fill Bioreactor Cultivation Strategy

Gahlawat¹,

Srivastava²

2018

Chem.Biochem.Eng.Q.

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“…The method of esterification of PHB for GC analysis was followed according to the protocol described by Riis and Mai (RIIS;MAI, 1988). 20-40 mg of freeze dried bacterial cells was weighed and put into a 10 mL tightly sealable vial.…”

Section: Methodsmentioning

confidence: 99%

A purple non-sulfur bacterium producing polyhydroxybutyrate and the conserved region of pha synthase gene

2016

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ABSTRACT:This study aimed to screen purple non-sulfur bacteria capable of accumulating granules or polyhydroxybutyrate (PHB) inside the cells, identify the potent strain, assay the enzyme or PHA synthase, and compare the PHB synthase gene with that of related strains. A total of 58 strains of purple non-sulfur bacteria were isolated from 108 samples of chicken feces in the chicken-egg farm of the Department of Animal Science, Faculty of Natural Resources at Prince of Songkla University, Hat Yai, Thailand. After cultivating the bacteria in glutamate malate (GM) medium without added glutamic acid under light (3,000 Lux) at 35 o C for 5 days, the intracellular biopolymer granules of the bacteria were observed by using a Confocal Laser Scanning Microscope (CLSM) with excitation and emission wavelength of 530 and 605 nm, respectively. Gas chromatography (GC) was carried out for quantitative analysis of PHB. There were five strains, CH12, CH52, CH72, CH90 and CH92, showed biopolymer granules under CLSM, and accumulated PHB 5, 1.7, 1.5, 1.4 and 1.8% (w w -1 ) of the cell dry weight (CDW), respectively. The 16S rDNA sequence analysis of CH12 strain showed a high homology of 100% correlation to that of Rhodopseudomonas palustris strain NCIB8288. Regarding the taxonomic characteristics and 16S rDNA sequence analysis, CH12 strain was identified as Rps. palustris NCIB8288. The PHA synthase activity of the crude extract from CH12 strain was 25 units/mL. The conserved regions could be aligned and selected among 5 strains of Rhodopseudomonas palustris (strains BisA53, TIE-1, CGA009, HaA2 and BisB18). The purified PCR product was obtained for further studies.

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“…The Dubois method was applied for carbohydrate determination (Dubois et al 1956) and phospholipids were determined according to a procedure developed for biofilm (Michaud et al 2003). Polyhydroxybutyrate was determined using the gas-chromatographic method (Riis and Mai, 1988). Ammonium, nitrite and nitrate were measured by ionic chromatography (Shimadzu, HIC-6A, Japan).…”

Section: Methodsmentioning

confidence: 99%

Macromolecular composition and anaerobic degradation of the sludge produced in a sequencing batch reactor

Chelme¹,

Fonseca²,

Mercado³

et al. 2008

Electron. J. Biotechnol.

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Gas chromatographic determination of poly-β-hydroxybutyric acid in microbial biomass after hydrochloric acid propanolysis

Cited by 323 publications

References 4 publications

Enhancing the Production of Polyhydroxyalkanoate Biopolymer by Azohydromonas Australica Using a Simple Empty and Fill Bioreactor Cultivation Strategy

Enhancing the Production of Polyhydroxyalkanoate Biopolymer by Azohydromonas Australica Using a Simple Empty and Fill Bioreactor Cultivation Strategy

A purple non-sulfur bacterium producing polyhydroxybutyrate and the conserved region of pha synthase gene

Macromolecular composition and anaerobic degradation of the sludge produced in a sequencing batch reactor

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