Gas chromatographic determination of thymol

Noall, Matthew W.; Knight, Vernon; Hargrove, William W.; Elledge, Barry

doi:10.1016/0003-2697(75)90559-x

“…So, the recommended method is well suited for the assay and evaluation of Thymol in mouth wash preparation and can also be considered as a general method for the quantification of Thymol. In comparison of the other methods [2][3][4][5][6][7][8][9][10][11][12] with FIA procedure, the later is more convenient than the former method because of its speed (sample through-put of 65 injection h −1 ) and wider linear range of the calibration graph. F-and t-test at 95% confidence level [16] showed that there was no significant difference between the proposed method and the standard Bp method during its application to the analysis of mouth washes samples Table (2 and 3).…”

Section: Discussionmentioning

confidence: 99%

“…It is used as an antiseptic lotion and mouth wash (1 in 1000) [1]. A number of analytical methods have been reported for the determination of Thymol, these included high pressure liquid chromatography [2][3][4], liquid chromatography with electrochemical detection [5], gas chromatography [6][7][8][9], differential-pulse voltammetry [10], ultraviolet spectrometry [11] and colorimetric analysis [12].…”

Section: Scheme(1): Structure Of Thymolmentioning

confidence: 99%

Flow Injection Spectrophotometric Determination of Thymol using 4-Aminoantipyrine and Copper(II) Nitrate

Al-Abachi¹,

Al-Najjar²

2015

Baghdad Sci.J

1

0

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Flow-injection (FI) spectrophotometric method has been developed for the analysis of thymol in pharmaceutical preparations. The method is based on organic coupling reaction between thymol and 4-amino antipyrine in the presence of alkaline medium to form an intense stable red color complex with copper nitrate that has a maximum absorption at 490 nm. Optimum conditions for determination of the drug was investigated .The calibration graph was linear over the range of 5-500 µg.ml-1 of thymol . The limit of detection (LOD) and limit of quantification (LOQ) were 1.81 ?g mL-1 and 3.60 ?g mL-1 respectively .The proposed method was applied satisfactorily to the determination of thymol in mouth wash preparations. The procedure is characterized by its simplicity, accuracy and precision.

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“…So, the recommended method is well suited for the assay and evaluation of Thymol in mouth wash preparation and can also be considered as a general method for the quantification of Thymol. In comparison of the other methods [2][3][4][5][6][7][8][9][10][11][12] with FIA procedure, the later is more convenient than the former method because of its speed (sample through-put of 65 injection h −1 ) and wider linear range of the calibration graph. F-and t-test at 95% confidence level [16] showed that there was no significant difference between the proposed method and the standard…”

Section: Discussionmentioning

confidence: 99%

“…It is used as an antiseptic lotion and mouth wash (1 in 1000) [1]. A number of analytical methods have been reported for the determination of Thymol, these included high pressure liquid chromatography [2][3][4], liquid chromatography with electrochemical detection [5], gas chromatography [6][7][8][9], differential-pulse voltammetry [10], ultraviolet spectrometry [11] and colorimetric analysis [12].…”

Section: Scheme(1): Structure Of Thymolmentioning

confidence: 99%

Flow Injection Spectrophotometric Determination of Thymol using 4-Aminoantipyrine and Copper(II) Nitrate

Al-Abachi,

Al-Najjar

2015

Baghdad Sci.J

1

0

View full text Add to dashboard Cite

Flow-injection (FI) spectrophotometric method has been developed for the analysis of thymol in pharmaceutical preparations. The method is based on organic coupling reaction between thymol and 4-amino antipyrine in the presence of alkaline medium to form an intense stable red color complex with copper nitrate that has a maximum absorption at 490 nm. Optimum conditions for determination of the drug was investigated .The calibration graph was linear over the range of 5-500 µg.ml-1 of thymol . The limit of detection (LOD) and limit of quantification (LOQ) were 1.81 ?g mL-1 and 3.60 ?g mL-1 respectively .The proposed method was applied satisfactorily to the determination of thymol in mouth wash preparations. The procedure is characterized by its simplicity, accuracy and precision.

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“…Various "high-performance thin-layer chromatography (HPTLC)" approaches have also been utilized for determining thymol either alone or in combination with other phenolic pharmaceuticals in Unani polyherbal formulations, essential oils, and plant extracts [26][27][28][29]. For the determination of thymol in essential oils, dosage forms, and plant extracts, various "gas-chromatography mass-spectrometry (GC-MS)" technique was used [30][31][32][33]. The determination of thymol in conjunction with eucalyptol, camphor, and menthol in Greek thyme honey was done using a GC-flame ionization detection (GC-FID) method [34].…”

Section: Introductionmentioning

confidence: 99%

Determination of Thymol in Commercial Formulation, Essential Oils, Traditional, and Ultrasound-Based Extracts of Thymus vulgaris and Origanum vulgare Using a Greener HPTLC Approach

Foudah

¹

,

Shakeel

²

,

Alqarni

³

et al. 2022

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In the literature, greener analytical approaches for determining thymol in its commercial formulations, plant-based phytopharmaceuticals, and biological fluids are scarce. As a result, the goal of this study is to develop and validate a normal-phase “high-performance thin-layer chromatography (HPTLC)” method for determining thymol in commercial formulations, essential oils, traditional extracts (TE), and ultrasound-based extracts (UBE) of Thymus vulgaris and Origanum vulgare obtained from various geographical regions. The greener mobile phase for thymol analysis was a binary combination of cyclohexane and ethyl acetate (85:15, v/v). The derivatized densitometric analysis of thymol was carried out under visible mode at 530 nm utilizing anisaldehyde-sulfuric acid as a derivatizing/visualizing agent. In the 10–2000 ng/band range, the greener normal-phase HPTLC method was linear. Furthermore, for thymol analysis, the proposed analytical approach was simple, quick, inexpensive, accurate, precise, robust, sensitive, and greener. The thymol contents in commercial formulation were computed as 7.61% w/w. In general, the thymol contents were maximum in essential oils of T. vulgaris and O. vulgare compared to the other sample matrices studied. The thymol contents of TE of T. vulgaris and O. vulgare of different geographical regions were significantly low compared to their UBE extract. Using 12 distinct components of green analytical chemistry, the overall “analytical GREEnness (AGREE)” scale for the proposed analytical approach was computed 0.79, showing the good greener nature of the proposed analytical approach. Overall, the greener normal-phase HPTLC technique was found to be reliable for determining thymol in commercial formulations and plant-based phytopharmaceuticals.

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Gas chromatographic determination of thymol

Cited by 5 publications

References 4 publications

Flow Injection Spectrophotometric Determination of Thymol using 4-Aminoantipyrine and Copper(II) Nitrate

Flow Injection Spectrophotometric Determination of Thymol using 4-Aminoantipyrine and Copper(II) Nitrate

Flow Injection Spectrophotometric Determination of Thymol using 4-Aminoantipyrine and Copper(II) Nitrate

Determination of Thymol in Commercial Formulation, Essential Oils, Traditional, and Ultrasound-Based Extracts of Thymus vulgaris and Origanum vulgare Using a Greener HPTLC Approach

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