Gas Chromatographic Measurement of Plasma Estrogens Using an Electron Capture Detector

Wotiz, Herbert H.; Charransol, G.; Smith, Irmeli N.

doi:10.1016/0039-128x(67)90076-1

Cited by 62 publications

(6 citation statements)

References 6 publications

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“…Other authors who have used different methods (35)(36)(37)(38)(39)(40) have reported E 2 levels in the same range. However, the concentrations of Ei in peripheral plasma measured by other methods are all considerably higher (35,37,41) than those found in this study. There are so few reported values of estrogens in ovarian vein blood that comparisons are not justified (1,3,7,9).…”

Section: Comparison Of Ovarian and Peripheral Vein Steroids (Tables 3-6mentioning

confidence: 75%

Concentration of Unconjugated Estrogens, Androgens and Gestagens in Ovarian and Peripheral Venous Plasma of Women: The Normal Menstrual Cycle¹

Lloyd

Lobotsky

Baird

et al. 1971

The Journal of Clinical Endocrinology & Metabolism

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The concentrations of unconjugated estrone (Ei), estradiol (E 2 ), testosterone (T), androstenedione (A) and progesterone (P) were measured in peripheral and ovarian venous plasma collected from 18 healthy women on days 7-30 of the menstrual cycle at the time of elective tubal ligation. An additional 4 steroids, pregnenolone, 17a-hydroxyprogesterone (17-OHP), 20«-dihydroxyprogesterone (20-OHP) and dehydroepiandrosterone (DHEA), were measured in ovarian venous plasma. In 2 subjects, one in the follicular phase and one in the luteal phase, blood was obtained from both ovaries. There was evidence of secretion by the ovary of all the steroids measured. Of the Cis and Cio steroids, E2 and A, respectively, were secreted by the ovary in largest amounts. There was a wide range of concentration of each steroid in ovarian venous plasma collected at the same time of the cycle from different subjects. High concentrations of E 2 were associated with high levels of Ei, T and A. DHEA appeared to be higher in plasma from the less actively secreting ovary. Equally high values of all these steroids were found in specimens from both phases of the cycle. There clearly was secretion of progesterone in the luteal phase from the ovary containing the corpus luteum. In the subject where bilateral sampling was performed, the ovary that did not contain the corpus luteum secreted lesser, though still significant, amounts of P than the one containing the corpus luteum. In the follicular phase, there was a suggestion of secretion of P in 2 cases. In 3 of 4 subjects in the follicular phase in which 17-OHP was measured, it was increased; in one of these, 17-OHP was higher in blood from the ovary secreting the smaller amount of e3trogen. In the luteal phase, high levels of 17-OHP were found in some subjects. The relationship between 17-OHP and P or E 2 was not consistent. The range of concentrations of all the steroids in the peripheral plasma was much less than in the ovarian plasma. The ratios of A/T and E2/Ei in ovarian vein plasma were 4-6 times higher than in peripheral vein plasma. (J Clin Endocr 32: 155, 1971) M OST of the concepts concerning the endocrine events in the menstrual cycle have been deiived from observations

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Section: Comparison Of Ovarian and Peripheral Vein Steroids (Tables 3-6mentioning

confidence: 75%

Concentration of Unconjugated Estrogens, Androgens and Gestagens in Ovarian and Peripheral Venous Plasma of Women: The Normal Menstrual Cycle¹

Lloyd

Lobotsky

Baird

et al. 1971

The Journal of Clinical Endocrinology & Metabolism

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“…The first attempts to apply urinary techniques to blood, and in particular a spectrophotometric endpoint, were not notably success¬ ful. Some advance was registered by the use ofradioactive internal standards combined with fluorimetry (Nachtigall, Bassett, Hogsander, Slagle «fe Levitz, 1966) and both sensitivity and specificity were improved by gas chromatography (Wotiz, Charransol & Smith, 1967) but it was not until competitive protein-binding techniques were introduced (Corker «fe Naftolin, 1971) that reliable clinical studies became feasible.…”

Section: Introductionmentioning

confidence: 99%

Variability of Total Plasma Oestriol in Late Human Pregnancy

Masson¹,

Wilson²

1972

Journal of Endocrinology

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“…Because of their low specificity, color reactions lead to unclear or even contradictory results, especially during the period of low estrogen excretion before puberty. Methods developed later for measuring plasma estrogens, gas chromatography [2,19] or double isotope derivative technique [3], are not applicable in children and adolescents because of the large quantities of plasma necessary. The introduction of radioimmunologic methods in steroid analysis, using highly specified antibodies against estrogens, made it possible to determine these hormones on the picogram range in large series.…”

Section: Introductionmentioning

confidence: 99%

Plasma Estrogens in Childhood and Puberty under Physiologic and Pathologic Conditions

Bidlingmaier¹,

Wagner-Barnack²,

Butenandt³

et al. 1973

Pediatr Res

136

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Plasma concentrations of estrone and estradiol were determined by radioimmunoassay of cord blood from 17 neonates and of venous blood of 15 neonates, 61 boys and 69 girls of all developmental stages, 27 healthy women, 20 healthy men, and 89 male and female adolescents with different disorders of sexual development. In cord blood both estrone and estradiol averaged 15,000 pg/ml. Blood drawn from neonates shortly after birth showed levels between 300 and 500 pg/ml. These levels fell to 5-15 pg/ml within a few days after birth, which is the normal range for both sexes up to 7 years. Before puberty, estrone rises earlier in girls than in boys. The increase of estradiol follows the onset of puberty. Boys reach adult levels in stage IV, whereas girls in stage V still have lower estrogen levels than mature women. We did not find higher levels in 29 adolescents with gynecomastia than in normal adolescents at the same stage of puberty. We observed abnormally high estrogens in children with congenital adrenal hyperplasia, but, in spite of some very high levels, we did not observe premature thelarche or gynecomastia in children with this disease. Six females and 10 males with panhypopituitarism, aged from 10 to 19 years, revealed low values within the infantile range. Six boys between 5 and 14 years who suffered from anorchia had very low levels of estrogen, especially estradiol (which was nearly undetectable). In contrast to patients with functioning testicle tissue, the patients with anorchia did not show any increase of estrogens after stimulation with human chorionic gonadotropin. Abnormally low levels were found in females with Turner's syndrome, but only in those with 45, XO karyotype. Patients with 45, XO/46, XX, or 46, XXp~ karyotype had high levels of estrogen which corresponded to clinical signs of advanced puberty. Speculation As demonstrated by our results, the radioimmunologic method enables us to measure the low estrogen levels which are found in children. This will permit a more intensive examination of the pituitary-gonadal and pituitary-adrenal interrelation in the future.

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Gas Chromatographic Measurement of Plasma Estrogens Using an Electron Capture Detector

Cited by 62 publications

References 6 publications

Concentration of Unconjugated Estrogens, Androgens and Gestagens in Ovarian and Peripheral Venous Plasma of Women: The Normal Menstrual Cycle¹

Concentration of Unconjugated Estrogens, Androgens and Gestagens in Ovarian and Peripheral Venous Plasma of Women: The Normal Menstrual Cycle¹

Variability of Total Plasma Oestriol in Late Human Pregnancy

Plasma Estrogens in Childhood and Puberty under Physiologic and Pathologic Conditions

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Gas Chromatographic Measurement of Plasma Estrogens Using an Electron Capture Detector

Cited by 62 publications

References 6 publications

Concentration of Unconjugated Estrogens, Androgens and Gestagens in Ovarian and Peripheral Venous Plasma of Women: The Normal Menstrual Cycle1

Concentration of Unconjugated Estrogens, Androgens and Gestagens in Ovarian and Peripheral Venous Plasma of Women: The Normal Menstrual Cycle1

Variability of Total Plasma Oestriol in Late Human Pregnancy

Plasma Estrogens in Childhood and Puberty under Physiologic and Pathologic Conditions

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Product

Resources

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Concentration of Unconjugated Estrogens, Androgens and Gestagens in Ovarian and Peripheral Venous Plasma of Women: The Normal Menstrual Cycle¹

Concentration of Unconjugated Estrogens, Androgens and Gestagens in Ovarian and Peripheral Venous Plasma of Women: The Normal Menstrual Cycle¹