Gas chromatographic method for the determination of fluoride ion in biological samples. I. Fluoride level in monkey.

Ikenishi, Rikio; Kitagawa, Takayasu; Nishiuchi, Masanori; K, Takehara; Yamada, Hideo; Nishino, Ikuko; Umeda, Tsuneji; Iwatani, Kouji; Nakagawa, Yuzo; Sawai, Moriharu; Yamashita, Takeo

doi:10.1248/cpb.36.662

Cited by 10 publications

(2 citation statements)

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“…Comparing the developed procedure with other chromatographic methods, GC‐FID procedures exhibit derivatization and measurement times either shorter or at a comparable time scale and comparable LODs 29, 48. Conventional IC may suffer from frequently emphasized overlapping of fluoride and void volume peaks.…”

Section: Discussionmentioning

confidence: 99%

Determination of fluoride as fluorosilane derivative using reversed‐phase HPLC with UV detection for determination of total organic fluorine

Musijowski

Szostek

Koc

et al. 2010

J of Separation Science

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A quantitative method for fluoride determination using RP HPLC and UV detection was developed. The method is based on the reaction of fluoride with triphenylhydroxysilane in highly acidic conditions and extraction of the reaction product into n-heptane. Chromatographic conditions as well as the derivatization parameters were optimized. LOQ for fluoride ion was evaluated as 0.25 μM (4.75 ppb) and linear range of response up to 75 μM (1.42 ppm) was obtained. The method was developed as a part of a procedure designed for the determination of total organic fluorine in natural waters, using SPE on a carbon sorbent and sodium biphenyl reagent for the defluorination reaction. LOD for a model compound, perfluorooctanoic acid, calculated for the complete procedure with 2000-fold preconcentration, is 20 ppt (n=4). Initial results show feasibility of total organic fluorine determination for environmental purposes.

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Section: Discussionmentioning

confidence: 99%

Determination of fluoride as fluorosilane derivative using reversed‐phase HPLC with UV detection for determination of total organic fluorine

Musijowski

Szostek

Koc

et al. 2010

J of Separation Science

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“…The in vivo catabolism of radiolabeled compounds is well documented; for example, many [ 18 F]-labeled compounds are known to be catabolized in vivo into smaller species, including [ 18 F]fluoride [6, 7]. Only a few positron-emitting radiopharmaceuticals are not catabolized.…”

Section: Introductionmentioning

confidence: 99%

Improved detection and measurement of low levels of [18F]fluoride metabolized from [18F]-labeled pyrimidine nucleoside analogues in biological samples

Paolillo

Yeh

Mukhopadhyay

et al. 2011

Nuclear Medicine and Biology

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Introduction It is important to identify all circulating metabolites, including free fluoride, for accurate pharmacokinetic modeling of [18F]-labeled radiotracers. We sought to determine the most efficient method to detect and quantify low levels of free [18F]fluoride in biological samples. Methods Low levels of [18F]fluoride were analyzed using two methods: A) an ion-exchange cartridge and gamma counting; and B) radio-HPLC, to compare the detection limits of these two analytical methods. Twenty microliters of [18F]fluoride solution was loaded onto an ion-exchange cartridge then eluted with 20% MeCN/water (5 mL) and radioactivity trapped in the cartridge counted on a gamma counter. [18F]-Fluoride was also determined in plasma and urine from mice injected with [18F]-labeled thymidine analogues using method A. Results The detection sensitivity of method A was 9.4-fold higher than that of method B (0.075±0.004 nCi vs. 0.71±0.02 nCi). Using method A, [18F]fluoride was determined in plasma for [18F]FLT, [18F]FMAU, [18F]FEAU and N3-[18F]FPrT as 1.4±0.31% (n=4), 0.17±0.49% (n=3), 4.88±1.62% (n=3) and 12.94±0.48% (n=4), respectively. The amount of [18F]fluoride determined in the urine was 11.49±1.60% (n=4) from [18F]FLT, 5.36±2.34% (n=3) from [18F]FMAU, 13.57±1.96% (n=3) from [18F]FEAU, and 11.19±1.98% (n=4) from N3-[18F]FPrT. Conclusion Low levels of [18F]fluoride in biological samples can be detected and quantified using an ion-exchange cartridge and gamma counting. This methodology is simple, accurate and superior to the standard use of radio-HPLC on a C18 column for metabolite analysis; and it should be useful in pharmacokinetic modeling for animal imaging studies using an [18F]-labeled radiotracer and PET.

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Determination of urinary fluoride by ion chromatography

Michigami¹,

Kuroda²,

Ueda³

et al. 1993

Analytica Chimica Acta

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Gas chromatographic method for the determination of fluoride ion in biological samples. I. Fluoride level in monkey.

Cited by 10 publications

References 0 publications

Determination of fluoride as fluorosilane derivative using reversed‐phase HPLC with UV detection for determination of total organic fluorine

Determination of fluoride as fluorosilane derivative using reversed‐phase HPLC with UV detection for determination of total organic fluorine

Improved detection and measurement of low levels of [18F]fluoride metabolized from [18F]-labeled pyrimidine nucleoside analogues in biological samples

Determination of urinary fluoride by ion chromatography

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