Gas Chromatographie Determination of Subnanogram Amounts of the Juvenile Hormone Methyl (2E, 6E)-10,ll-epoxy-3,7,ll-tri- methyl-2,6-dodecadienoate (JH III) in Insect Material by Electron Capture Detection

Broekhoven, Laura Van; Kerk, A Van Der; Hoof, V O Van; Salemink, C. A.

doi:10.1515/znc-1975-11-1205

Cited by 11 publications

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“…Clearly it will not be possible to interpret the effects of these differing levels of CA activity on the various target tissues with any conviction until accurate measurements of the JH titres in the haemolymph have been made. Precise chemical measurement of JH titres in the haemolymph is now possible (van Broekhoven et al, 1975;Lanzrein et al, 1975;Bergot et al, 1976) and would be most helpful in the present context because of our finding that the effect of mating on the activity of cockroach CA is far from 'all or none'. The patterns of activity of the CA are essentially the same in females reared in the presence or absence of males up until day 6 of adult life, that is to say until 1-2 days after the females first become receptive to males.…”

Section: Relationship Between Corpus Allatum Activity and Reproductivmentioning

confidence: 99%

The effect of enforced virginity and subsequent mating on the activity of the corpus allatum of Periplaneta americana measured in vitro, as related to changes in the rate of ovarian maturation

Weaver

Pratt

1977

Physiological Entomology

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ABSTRACT. Female P. americana, reared with males from the time of adult emergence, mated on the 4th–5th day after metamorphosis, produced the first ootheca on the 8th or 9th day, and then produced successive oothecae at intervals of 3.0 days, whereas, only 50% of virgin females had produced their first ootheca by the 28th day after adult emergence. Examination of the ovaries indicated that oocyte development is normal in virgins until shortly after the time when they first become receptive to males. When mating was not allowed there was a dramatic reduction in the rate of vitellogenic growth of the terminal batch of oocytes which persisted until mating was allowed, and was often accompanied by resorption of a percentage of the oocytes. Short‐term, in vitro, radiochemical assay of juvenile hormone (JH III) biosynthesis by corpora allata (CA) showed that, in females reared with males, the cycles of ovarian development are accompanied by regular pulses of CA activity. There is a small, possibly preparatory peak of JH III biosynthesis before vitellogenesis of the first wave of oocytes, followed by a larger peak of JH III production during vitellogenesis of this batch of eggs and one peak of CA activity between ovulation of each subsequent wave of oocytes. Activities as low as 0.25 pmol C16JH/CA pair/h and as high as 48.38 pmol/CA pair/h were observed in CA from mated females after the onset of cyclic activity. Stimuli received during mating are somehow responsible for the cyclic activity of the CA, for when females were subjected to enforced virginity the first small peak was normal but the second peak was not fully realized and there was then a gradual decline in CA activity until approximately 2 weeks post‐emergence. Thereafter the glands exhibited a more or less constant rate of JH biosynthesis (mean = 3.45 ± 0.32 pmol/CA pair/h.) When females were mated after 21 days of enforced virginity the activity of the CA was enhanced. By 48 h after mating the mean glandular activity was at least four times that found in virgins of the same age, and by 72 h rates as high as 40 pmol/CA pair/h were observed. This was followed by normal cyclic activity of the CA. The increase in rate of JH biosynthesis appears to result in a recommencement of oocyte development in these ‘delayed‐mated’ females.

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Section: Relationship Between Corpus Allatum Activity and Reproductivmentioning

confidence: 99%

The effect of enforced virginity and subsequent mating on the activity of the corpus allatum of Periplaneta americana measured in vitro, as related to changes in the rate of ovarian maturation

Weaver

Pratt

1977

Physiological Entomology

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Analysis of insect juvenile hormones by gas chromatography mass spectrometry: Problems of sample preparation and choice of detection procedure

Mauchamp

Lafont

Hardy

et al. 1979

Biol. Mass Spectrom.

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In this paper we introduce a novel procedure for the analysis of insect juvenile hormones and describe the principal steps involved in this method. Due to the low concentration of these hormones in insects, careful purification of samples before analysis is necessary. Biological samples are purified by solvent partition and reversed phase high pressure liquid chromatography, then analysed by gas chromatography mass spectrometry using a spectrometer equipped with a data acquisition system. By this procedure lOOpg of insect juvenile hormone could be detected in biological extracts.

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A method for the quantitative determination of juvenile hormones in insects

Huibregtse-Minderhoud

Hoof

Wijkens

et al. 1979

Recl. Trav. Chim. Pays‐Bas

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Red. Trav. Chim. Pays-Bas 98 371 -424 (1979)/RTCPA3-98 (6) 371-424 (1979)/june 1979 A m e t h o d f o r t h e q u a n t i t a t i v e d e t e r m i n a t i o n o f j u v e n i l e h o r m o n e s i n i n s e c t s * Laborat,or-of 6rg(wic ,'hc.w:s$ry, 6+nte L'nigersYty, P. 0. BOX 5 P 5 5 , 350," c7.? L t r t 9 r F t , The Net herlands (RPiaeiiwd May 1 2 t h 1379) Abstract. A method to determine the content of the juvenile hormones in insects a s low as 0.2 nanogram per sample was developed. The juvenile hormones were transformed in their 10-hepta-' f l u o r o h u t y r a t e -l l -m e t h o x y -d e r i v a t i v e s and simultaneously determined by capillary gas chromato-

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Gas Chromatographie Determination of Subnanogram Amounts of the Juvenile Hormone Methyl (2E, 6E)-10,ll-epoxy-3,7,ll-tri- methyl-2,6-dodecadienoate (JH III) in Insect Material by Electron Capture Detection

Cited by 11 publications

References 0 publications

The effect of enforced virginity and subsequent mating on the activity of the corpus allatum of Periplaneta americana measured in vitro, as related to changes in the rate of ovarian maturation

The effect of enforced virginity and subsequent mating on the activity of the corpus allatum of Periplaneta americana measured in vitro, as related to changes in the rate of ovarian maturation

Analysis of insect juvenile hormones by gas chromatography mass spectrometry: Problems of sample preparation and choice of detection procedure

A method for the quantitative determination of juvenile hormones in insects

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