Gas Chromatography as a Potential Means of Diagnosing Arthritis. I. Differentiation between Staphylococcal, Streptococcal, Gonococcal, and Traumatic Arthritis

Brooks, John B.; Kellogg, Douglas S.; Alley, C C; Short, Helen B.; Handsfield, H. Hunter; Huff, Baker

doi:10.1093/infdis/129.6.660

Cited by 51 publications

(10 citation statements)

References 9 publications

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“…GLC techniques are used routinely in the speciation of anaerobic organisms (2) and have been reported to be useful in the detection of bacteria in blood cultures (3) and other body fluids (4), including septic synovial fluid (5). The identification of bacteria is made possible by the presence of characteristic bacterial metabolites, including short-chain fatty acids (SCFA), which can be detected and quantified by GLC.…”

Section: Gas-liquid Chromatographic Analysis Of Synovial Fluidmentioning

confidence: 99%

Gas‐liquid chromatographic analysis of synovial fluid. Succinic acid and lactic acid as markers for septic arthritis

Borenstein

Gibbs

Jacobs

1982

Arthritis & Rheumatism

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Nonvolatile short-chain fatty acids from 80 synovial fluids were quantified by gas-liquid chromatography. Succinic acid was detectable in all 23 septic synovia1 fluids infected with either gram-positive or gramnegative organisms and in only 5 of 57 nonseptic synovial fluids. Lactic acid was present in all of the effusions but was correlated with septic arthritis only when present in concentrations greater than 250 mg% .Neither short-chain fatty acid was more sensitive than high white blood cell counts (>50,000 mm3) or depressed glucose concentration (<40 mg/dl) in diagnosing septic arthritis before antibiotic therapy; however, the detection of succinic acid was helpful in identifying patients with septic arthritis who had been given antibiotic treatment before arthrocentesis. Thus, gas-liquid chromatography, a rapid and sensitive method for the detection of short-chain fatty acids, may complement the currently available methods used to diagnose septic arthritis.

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Section: Gas-liquid Chromatographic Analysis Of Synovial Fluidmentioning

confidence: 99%

Gas‐liquid chromatographic analysis of synovial fluid. Succinic acid and lactic acid as markers for septic arthritis

Borenstein

Gibbs

Jacobs

1982

Arthritis & Rheumatism

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“…Mitruka and co-workers (16)(17)(18)(19)(20) have analyzed serum samples with GLC to diagnose experimental viral and bacterial infections. A recent report by Brooks et al (21) has suggested that GLC might be useful in the differential diagnosis of arthritis.…”

Section: Resultsmentioning

confidence: 99%

Rapid diagnosis of anaerobic infections by direct gas-liquid chromatography of clinical speciments.

Gorbach¹,

Mayhew²,

Bartlett³

et al. 1976

J. Clin. Invest.

165

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“…Furthermore, to trust the test results, one requires the technician to have a great deal of experience. The diagnosis of tuberculosis by culture studies is time consuming, the culturing time required for isolation of M. tuberculosis being [3][4][5][6] wk (23).…”

Section: Discussionmentioning

confidence: 99%

Demonstration of tuberculostearic acid in sputum from patients with pulmonary tuberculosis by selected ion monitoring.

Odham¹,

Larsson²,

Mårdh³

1979

J. Clin. Invest.

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A B S T R A C T Selected ion monitoring was used to d-etect tuberculostearic acid (1O-methyloctadecanoic acid) in sputum from patients with pulmonary tuberculosis. The specimens were autoclaved, lyophilized, extracted, and methanolysed before being subjected to thin-layer chromatography and injected into the gas chromatograph/mass spectromieter. Tuberculostearic acid could be detected in five of six tuberculous sputum specime'ns containing acid-fast rods detectable by light microscopy of Ziehl-Neelsen stained smears. After the sputum specimens had been cultured for five days on Lowenstein-Jensen medium, when still no colonies could be observed visually, the presence of tuberculostearic acid was demonstrated in a'll six cases of tuberculosis.In corresponding analyses of sputum from eight patients with non-tuberculous pneumonia, tuberculostearic acid was not fou'nd. This fatty acid, the presence of which was also demonstrated in cultures of various mycobacterial and nocardial species, is characteristic of organisms of the order Actinomycetales. The demonstration of tuberculostearic acid in sputum specimens may constitute a rapid and sensitive way of diagnosing pulmonary tuberculosis.

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Gas Chromatography as a Potential Means of Diagnosing Arthritis. I. Differentiation between Staphylococcal, Streptococcal, Gonococcal, and Traumatic Arthritis

Cited by 51 publications

References 9 publications

Gas‐liquid chromatographic analysis of synovial fluid. Succinic acid and lactic acid as markers for septic arthritis

Gas‐liquid chromatographic analysis of synovial fluid. Succinic acid and lactic acid as markers for septic arthritis

Rapid diagnosis of anaerobic infections by direct gas-liquid chromatography of clinical speciments.

Demonstration of tuberculostearic acid in sputum from patients with pulmonary tuberculosis by selected ion monitoring.

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