Gas Chromatography-Mass Spectrometry Analysis and in Vitro Antimicrobial Screening of Wedelia Glauca (Ortega) O. Hoffm. Ex Hicken

Abstract

Objective: Continued resistance toward the antibiotics urges us to explore newer antibiotics. Plants are being the safer source of antibiotics with lesser or no side effects. This study was designed to study the presence of phytochemical constituents and antibacterial activity of leaf and flower extracts of Wedelia glauca against urinary tract infection causing pathogens. Methods:The plant leaves were extracted with five different solvents based on the polarity. The extraction was done using soxhalation. Antimicrobial activity was determined by agar well diffusion method for both the sample and standard. The acetone plant extract was subjected to gas chromatography-mass spectrometry (GC-MS) analysis for screening phytoconstituents.Results: Preliminary phytochemical screening revealed the presence of diverse phytoconstituents in the plant. The different extracts exhibited a considerable antimicrobial potential. Among the solvents used acetone extract showed comparably better antimicrobial activity with 100% of inhibition rate with the maximum zone of inhibition of 1.6±0.77 mm against Staphylococcus sp. and Aspergillus sp. at the concentration of 5 mg. GC-MS analysis provided 8 major peaks which revealed the existence of a variety of bioactive compounds which may attribute to the efficacy of the plant. Conclusion:W. glauca leaf and flower extracts displayed a broad spectrum of antibacterial and antifungal activity and can be considered as a potential source of newer antibiotic compounds.

Bioactivity and metabolomics fingerprinting characterization of different organic solvents extracts of Padina pavonica collected from Abu Qir Bay, Egypt

“…The acetone showed the highest percentage as an antioxidant (80.58%), followed by ethanol (74.51%) and then ethyl acetate (69.66%). Because the bioactive compounds in acetone and ethanol extracts (phenols, flavonoids, carotenoids, and tannins), are higher than those in ethyl acetate extract responsible for the antioxidant activity, and that in Agreement With Several published Reports For Examples [57][58] Pigments such as carotenoids and chlorophyll, and vitamin precursors including carotene, ascorbic acid, thiamine, niacin, and phenolic substances such as hydroquinone, polyphenols, and flavonoids may contribute to the antioxidant activity of marine algae. Phospholipids, especially peptides, terpenoids, phosphatidylcholine, and other antioxidant compounds played a role in inhibiting or suppressing oxidation processes, either indirectly or directly [18].…”