Gas chromatography/mass spectrometry and gas chromatography/mass spectrometry/mass spectrometry of methyl ester/methoxime/trimethylsilyl ether derivatives of thromboxanes

Abstract

Electron impact mass spectra of methyl ester/methoxime/trimethylsilyl ether derivatives of thromboxane B2 (TxB2) and 2,3-dinor-TxB2 and methyl ester/trimethylsilyl ether derivative of 11-dehydro-TxB2 are presented. Additionally, the derivatives of (2H4)-thromboxanes and methyl ester (2H3)-methoxime/trimethylsilyl ether derivatives of TxB2 and 2,3-dinor-TxB2 and (2H3)-methyl ester/trimethylsilyl ether derivative of 11-dehydro-TxB2 were investigated. Collision-induced dissociation mass spectra of the most intense parent ion in the high-mass region were taken. Collisionally activated decomposition mass spectra of the [C(12)-C(20)]+ ion of TxB2 and 2,3-dinor-TxB2 show an intense but not specific daughter ion, whereas the collision-induced dissociation mass spectrum of the [M-(C(16)-C(20)]+ ion of 11-dehydro-TxB2 results in the formation of numerous daughter ions.

Fast atom bombardment and collision-induced dissociation of prostaglandins and thromboxanes: Some examples of charge remote fragmentation

“…Previous MS/MS studies of prostaglandins and thromboxanes centered on the cm of positive ions generated either by EI of the methyl ester trimethylsilyl (and methoxime derivatives of keto groups) derivatives [18][19][20][21][22] or of the pentafluorobenzyl ester, trimethylsilyl ether derivatives [23]. In the latter investigations, negative ion electron capture chemical ionization mass spectrometry (ClMS) was employed.…”