Gas Chromatography–Mass Spectrometry-Based Metabolite Profiling for the Assessment of Freshness in Gilthead Sea Bream (Sparus aurata)

Mallouchos, Athanasios; Mikrou, Theano; Gardeli, Chrysavgi

doi:10.3390/foods9040464

Cited by 20 publications

(7 citation statements)

References 29 publications

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“…Compared with fresh samples, the upregulated metabolites of the C group at day 18 were N-acetyl-L-phenylalanine, pipecolic acid, ornithine, with the extend of storage time, the concentration of amino acids such as leucine, tyrosine, and methionine were increased because of autolysis and bacterial spoilage. 45 However, the upregulated metabolite of the LVEF-PF group at day 18 was benzyl isothiocyanate, and the down-regulated metabolites in LVEF-PF group were L-arginine, γ-L-glutamyl-L-cysteinyl-⊎-alanine. The major differential metabolites of the PF group on day 18 were all the downregulated metabolites, containing ornithine, pipecolic acid.…”

Section: Metabolic Changes In Large Yellow Croaker With Different Tre...mentioning

confidence: 92%

Effect of low‐voltage electrostatic field‐assisted partial freezing on large yellow croaker protein properties and metabolomic analysis during storage

Fei,

Ma,

Yue

et al. 2023

J Sci Food Agric

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BackgroundLarge yellow croaker is highly perishable during storage because of high protein and moisture content. The degradation of the fish is mainly attributed to microbial growth and enzyme activity, so it is important to find an efficient storage method to extend its shelf life.MethodologyThis study investigated the effect of a low‐voltage electrostatic field combined with partial freezing treatment on the physicochemical properties of myofibrillar protein (MP) and metabolomic analysis of large yellow croaker during preservation. The samples in chilled storage (C), partial freezing storage (PF) and 6 kV/m low‐voltage electrostatic field partial freezing storage (LVEF‐PF) were analyzed during an 18 day storage period.ResultsIn comparison with the C and PF groups, LVEF‐PF delayed the oxidation of MP by inhibiting the formation of carbonyl groups (2.25 nmol/mg pro), and maintaining higher sulfhydryl content (29.73 nmol/mg pro). Fourier transform infrared (FTIR) spectroscopy and fluorescence spectroscopy analysis also demonstrated that the LVEF‐PF treatment maintained the stability of the protein structure by increasing the a‐helix ratio (19.88%) and reducing the random coil ratio (17.83%). Scanning electron microscopy showed that, compared with the LVEF‐PF group, there was more degeneration and aggregation of MP in the C and PF groups after 18 days’ storage. The results of untargeted metabolomic analysis showed that 415 kinds of differential metabolites were identified after storage, and the difference levels of differential metabolites were least between the samples treated with LVEF‐PF stored on the ninth day and the fresh samples. The main differential metabolic pathways during storage were amino acid metabolism and lipid metabolism.ConclusionThe LVEF‐PF treatment could maintain the stability of myofibrillar protein in large yellow croaker during storage. These results showed a potential application of the LVEF‐PF method for aquatic product preservation. © 2023 Society of Chemical Industry.

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Section: Metabolic Changes In Large Yellow Croaker With Different Tre...mentioning

confidence: 92%

Effect of low‐voltage electrostatic field‐assisted partial freezing on large yellow croaker protein properties and metabolomic analysis during storage

Fei,

Ma,

Yue

et al. 2023

J Sci Food Agric

View full text Add to dashboard Cite

show abstract

“…GC coupled with MS (GC–MS) is frequently used to identify and quantify odorous compounds of food products in the food industry. As the conventional method, GC–MS is used for fish freshness determination applications that are based on the odorous compounds of fish (Dehaut et al., 2016; Mallouchos et al., 2020). Therefore, it is important to focus on the comparison between the GC–MS and e‐noses.…”

Section: Current Applications Of Artificial Senses For Fish Freshness...mentioning

confidence: 99%

Current applications and future trends of artificial senses in fish freshness determination: A review

Madhubhashini,

Liyanage,

Alahakoon

et al. 2023

Journal of Food Science

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Fish is a highly demanding food product and the determination of fish freshness is crucial as it is a fundamental factor in fish quality. Therefore, the fishery industry has been working on developing rapid fish freshness determination methods to monitor freshness levels. Artificial senses that mimic human senses are developed as convenient emerging technologies for fish freshness determination. Computer vision, electronic nose (e‐nose), and electronic tongue (e‐tongue) are the emerging artificial senses for fish freshness determination. This review article is uniquely worked upon to investigate the current applications of the artificial senses in fish freshness determination while describing the steps, and fundamental principles behind each artificial sense, comparing them with their advantages and limitations, and future trends related to fish freshness determination. Among the artificial senses, computer vision determines the freshness of fish in a completely nondestructive way while the e‐tongue determines the freshness of fish in a completely destructive way. There are developed e‐noses for fish freshness determination in both destructive and nondestructive ways. By analyzing visual cues such as color, computer vision systems can assess fish quality without the need for physical contact and it makes computer vision suitable for large‐scale industrial fish quality assessing applications. Overall, this review study reveals artificial senses as a proven replacement for traditional sensory panels in determining fish freshness precisely and conveniently. As future trends, there is a demand for developing applications for consumers to determine fish freshness based on artificial senses.

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“…Mass spectra can be easily compared with available online libraries [ 155 ] and databases such as the National Institute of Advanced Industrial Science and Technology (AIST) spectral library and the National Institute of Standards and Technology (NIST) library or, more commonly, via in-house libraries [ 156 , 157 ]. The GC-MS has been used to track changes in the fraction of polar metabolites of sea bream [ 158 ] and identify phytoconstituents present in the root exudates of Corbichonia decumbens [ 159 ] and chemometric profiles of root extracts of Rhodiola imbricata [ 160 ]. Increased spatial resolution of sampling combined with GC-MS-based analysis characterized four fungal endophytes from the genus Aspergillus and seven bacterial endophytes from the Kocuria , Bacillus , Arthrobacter , Staphylococcus , and Micrococcus genera [ 161 ].…”

Section: Metabolomics: the Bridge Of Multi-omics In Metabolite Discov...mentioning

confidence: 99%

Tapping into Plant–Microbiome Interactions through the Lens of Multi-Omics Techniques

Mishra

Sudalaimuthuasari

Hazzouri

et al. 2022

Cells

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This review highlights the pivotal role of root exudates in the rhizosphere, especially the interactions between plants and microbes and between plants and plants. Root exudates determine soil nutrient mobilization, plant nutritional status, and the communication of plant roots with microbes. Root exudates contain diverse specialized signaling metabolites (primary and secondary). The spatial behavior of these metabolites around the root zone strongly influences rhizosphere microorganisms through an intimate compatible interaction, thereby regulating complex biological and ecological mechanisms. In this context, we reviewed the current understanding of the biological phenomenon of allelopathy, which is mediated by phytotoxic compounds (called allelochemicals) released by plants into the soil that affect the growth, survival, development, ecological infestation, and intensification of other plant species and microbes in natural communities or agricultural systems. Advances in next-generation sequencing (NGS), such as metagenomics and metatranscriptomics, have opened the possibility of better understanding the effects of secreted metabolites on the composition and activity of root-associated microbial communities. Nevertheless, understanding the role of secretory metabolites in microbiome manipulation can assist in designing next-generation microbial inoculants for targeted disease mitigation and improved plant growth using the synthetic microbial communities (SynComs) tool. Besides a discussion on different approaches, we highlighted the advantages of conjugation of metabolomic approaches with genetic design (metabolite-based genome-wide association studies) in dissecting metabolome diversity and understanding the genetic components of metabolite accumulation. Recent advances in the field of metabolomics have expedited comprehensive and rapid profiling and discovery of novel bioactive compounds in root exudates. In this context, we discussed the expanding array of metabolomics platforms for metabolome profiling and their integration with multivariate data analysis, which is crucial to explore the biosynthesis pathway, as well as the regulation of associated pathways at the gene, transcript, and protein levels, and finally their role in determining and shaping the rhizomicrobiome.

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Gas Chromatography–Mass Spectrometry-Based Metabolite Profiling for the Assessment of Freshness in Gilthead Sea Bream (Sparus aurata)

Cited by 20 publications

References 29 publications

Effect of low‐voltage electrostatic field‐assisted partial freezing on large yellow croaker protein properties and metabolomic analysis during storage

Effect of low‐voltage electrostatic field‐assisted partial freezing on large yellow croaker protein properties and metabolomic analysis during storage

Current applications and future trends of artificial senses in fish freshness determination: A review

Tapping into Plant–Microbiome Interactions through the Lens of Multi-Omics Techniques

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