2024
DOI: 10.1111/asj.13896
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Gas chromatography–mass spectrometry‐based quantitative method using tert‐butyldimethylsilyl derivatization for plasma levels of free amino acids and related metabolites in Japanese Black cattle

Hanwool Do,
Emi Yoshida,
Tatsunori Masaki
et al.

Abstract: The quantification of amino acid and related metabolite levels is important for evaluating amino acid metabolism and function in animals. However, a useful quantitative method is not enough. In this study, we developed and validated tert‐butyldimethylsilyl derivatization method using gas chromatography–mass spectrometry to quantify plasma levels of free amino acids and related metabolites in Japanese Black cattle. Of the 51 metabolites examined, 24, including 20 amino acids, one amine, and three keto acids, co… Show more

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