Gas chromatography-mass spectrometry determination of matrine in human plasma

Sit, Daniel

doi:10.1016/j.jchromb.2004.05.010

Cited by 35 publications

(22 citation statements)

References 4 publications

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“…Pure matrine was fine white powders that dissolved readily in water and common organic solvents. Matrine-14, 14-d 2 was synthesized in our laboratory using a chemical reaction that could replace Ͼ97% of the hydrogen at position 14 of the matrine molecule with deuterium (Sit et al, 2004). ACAPHA was a gift from Global Cancer Strategies Ltd. (British Columbia, Canada); it was a mixture of dark brown powders and amorphous solids.…”

Section: Methodsmentioning

confidence: 99%

“…Preliminary studies indicated that matrine equilibrium was established between the blood and the plasma in 20 min under these experimental conditions. Matrine concentrations in the plasma samples were measured by GC/MS (Sit et al, 2004). The concentrations in blood were assumed to be the theoretical concentration.…”

Section: Methodsmentioning

confidence: 99%

“…Plasma/tissue samples were extracted and quantified for matrine according to the procedure of Sit et al (2004). In brief, approximately 1.0 g of tissue sample was weighed and homogenized in 3 ml of distilled water with a Kinematica homogenizer (PCU-2-110; Kinematica, Littau-Lucerne, Switzerland).…”

Section: Methodsmentioning

confidence: 99%

“…These chemicals also are capable of stimulating immunological activity and inhibiting tumor growth in humans (Chang, 1992;Xu and Jiang, 1998). 2) Although matrine constitutes Ͻ2% (w/w) of the S. tonkinensis roots, it is the only alkaloid found in the plasma/tissues of rats after ACAPHA administration (Sit et al, 2004;Gao, 2007). 3) Specific and sensitive analytical methods, such as gas chromatography (GC)/mass spectrometry (MS) (Sit et al, 2004), high-performance liquid chromatography/UV (Wu et al, 2003), and high-performance liquid chromatography/MS (Wang et al, 2005), have been developed to detect and quantify matrine in biological samples.…”

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confidence: 99%

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Physiologically Based Pharmacokinetics of Matrine in the Rat after Oral Administration of Pure Chemical and ACAPHA

Gao

Law²

2009

Drug Metab Dispos

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ABSTRACT:ACAPHA, a botanical drug for the treatment of human esophageal cancer in China, is under investigation as a lung cancer chemoprevention agent at the BC Cancer Agency (Vancouver, BC, Canada). Little or no information is available on the pharmacokinetics of ACAPHA in animals. The objectives of this study were as follows: to examine the disposition kinetics of matrine, a bioactive marker of ACAPHA in the rat; to develop a physiologically based pharmacokinetic (PBPK) model for pure matrine; and to characterize the absorption and clearance of crude matrine in ACAPHAtreated rats using the PBPK model. Pure matrine (15 mg/kg) or crude matrine in the form of ACAPHA (0.38 or 3.8 g/kg) was administered to the rat by gavages. The rats were sacrificed at different time points postdosing. Blood and major organs were removed from the rat, extracted with toluene/butanol, and quantified for matrine using gas chromatography-mass spectrometry. An 11-compartment, flow-limited PBPK model of matrine was developed. The PBPK model was able to simulate closely the empirical data of rats treated with pure matrine. Because the absorption and clearance of crude matrine in ACAPHA-treated rats could not be parameterized a priori, they were estimated by fitting the experimental data to the PBPK model. Results of the study show that pure matrine is absorbed and eliminated by the rat at faster rates than crude matrine. Moreover, the ACAPHA matrix may change the pharmacokinetics of matrine in the rat significantly. The PBPK model is a valuable tool to gain insights into the disposition kinetics of a botanical drug.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Physiologically Based Pharmacokinetics of Matrine in the Rat after Oral Administration of Pure Chemical and ACAPHA

Gao

Law²

2009

Drug Metab Dispos

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“…[2][3][4] Therefore, it is necessary to determine and control the contents in biological samples, such as plasma and urine. Several methods have been developed for the determination of matrine or sophocarpine including high-performance capillary electrophoresis (HPCE), 5 high-performance liquid chromatography (HPLC), 6 gas chromatography-mass spectrometry (GC-MS) 7 and liquid chromatography-mass spectrometry (LC-MS). 8 But most of them utilize plasma protein precipitation to delete the matrix disturbance, which have low accuracy and are difficult to operate.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Matrine Alkaloids in Human Urine by Hollow Fiber Liquid-phase Microextraction with High-performance Liquid Chromatography

Han¹,

Row²

2010

Journal of the Korean Chemical Society

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의 루멘에 pH 1.50의 100 mmol/L of H3PO4 수용체 용액을 주입한다, 1 mol/L NaOH는 공급 용액의 pH, 600 rpm의 교반속도와 60분의 추출시간을 조정하여 사용된다. LPME의 방법은 실제의 소변 샘플에서 마트린과 소포카르핌의 분석에 성공적으로 적용되었다. 주제어: 중공섬유 액상 미세추출, 인간 뇨, Matrine Alkaloids ABSTRACT.A sensitive quantitative method for the determination of matrine alkaloids in human urine was developed based on hollow fiber liquid-phase microextraction (HF-LPME) combined with HPLC. The influence of the different factors on the HF-LPME efficiency including the pH and ion strength of the donar solution, the pH of the acceptor solution, stirring rate and extraction time were examined. The best HF-LPME conditions were as follows: 1-octanol impregnated in the pores of the hollow fiber, 100 mmol/L of H3PO4 at pH 1.50 as the acceptor solution injected into the lumen of the hollow fiber, 1 mol/L NaOH used to adjust the pH of the donor solution, stirring rate of 600 rpm and extraction time of 60 min. The LPME method was applied successfully to the analysis of matrine and sophocarpine in real urine samples.

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An HPLC method for the determination and pharmacokinetic study of lehmannine in rat plasma

Zhao

Yuhui

et al. 2008

Biomedical Chromatography

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A high-performance liquid chromatographic (HPLC) method for determining lehmannine (LMN) in rat plasma was developed for application in the pharmacokinetics study. The plasma was deproteinized with acetonitrile that contained an internal standard and was separated from the aqueous layer by adding sodium chloride. The HPLC assay was carried out using a VP-ODS column at 40 degrees C. The mobile phase was acetonitrile-0.02 mol/L ammonium acetate buffer-triethylamine (35:65:0.04, v/v/v). The flow rate was 1.0 mL/min. The detection wavelength was set at 220 nm. The method was used to determine the concentration-time profiles of LMN in the plasma following oral administration or bolus injection of LMN aqueous solution. The pharmacokinetic parameters of LMN were calculated for the first time by Drug and Statistics 1.0 program.

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Gas chromatography-mass spectrometry determination of matrine in human plasma

Cited by 35 publications

References 4 publications

Physiologically Based Pharmacokinetics of Matrine in the Rat after Oral Administration of Pure Chemical and ACAPHA

Physiologically Based Pharmacokinetics of Matrine in the Rat after Oral Administration of Pure Chemical and ACAPHA

Analysis of Matrine Alkaloids in Human Urine by Hollow Fiber Liquid-phase Microextraction with High-performance Liquid Chromatography

An HPLC method for the determination and pharmacokinetic study of lehmannine in rat plasma

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