Gas Chromatography-Mass Spectrometry (GC-MS) and evaluation of antioxidant and antimicrobial activities of essential oil of Campomanesia adamantium (Cambess.) O. Berg (Guavira)

Abstract

The essential oils from Campomanesia adamantium (Cambess.) O. Berg leaves, collected in the reproductive (flowering and fruit-bearing) and vegetative stages, were characterized by GC-MS (Gas Chromatography-Mass Spectrometry). A total of 95 compounds of the essential oils were identified. In the reproductive stage (flowering) the major constituents were monoterpenes (limonene, α-pinene and β-pinene) while during the vegetative stage the major constituents were the sesquiterpenes (bicyclogermacrene and globulol). The essential oil of the reproductive stage shows high antimicrobial activity against Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans, and all show moderate activity against Escherichia coli. The essential oils were also evaluated for their radicalscavenging activity by DPPH. The chemogeographical variations of the oil composition from the four distinct localities studied all contained α-pinene, β-pinene, limonene, linalool, β-caryophyllene, germacrene D and bicyclogermacrene, however the samples from Jardim city contained neither limonene nor linalool. Uniterms:Campomanesia adamantium/antioxidant activity. Campomanesia adamantium/antimicrobial activity. Guavira. Essential oil/caracterization.Os óleos essenciais obtidos das folhas de Campomanesia adamantium foram caracterizados através da combinação de CG-EM e índice de retenção, sendo identificado um total de 95 compostos. Na floração as substâncias majoritárias foram monoterpenos (limoneno, α-pineno e β-pineno) e durante o estágio vegetativo as substâncias majoritárias foram sesquiterpenos (biciclogermacreno e globulol). Os óleos essenciais obtidos da floração e frutificação mostraram alta atividade contra Staphylococcus aureus, Pseudomonas aeruginosa e Candida albicans e moderada contra Escherichia coli em todos os estágios. Foi avaliada a atividade antioxidante dos óleos essenciais usando o método do DPPH. O óleo essencial das 4 cidades mostrou a presença de α-pineno, β-pineno, limoneno, linalol, β-cariofileno, germacreno D e biciclogermacreno, mas a amostra da cidade de Jardim não apresentou limoneno e linalol.Unitermos: Campomanesia adamantium/atividade antioxidante. Campomanesia adamantium/atividade antimicrobiana. Guavira. Óleo essencial/caracterização.

Antimicrobial activity of plants used as medicinals on an indigenous reserve in Rio das Cobras, Paraná, Brazil

“…Campomanesia adamantum also had activity against Candida albicans (Bonilla et al, 2005;Coutinho et al, 2009;Pavan et al, 2009). No reports of the antifungal activity of Luehea paniculata and others species of the same genus were found in the literature.…”

Atividades biológicas dos óleos essenciais de Endlicheria citriodora, uma lauraceae rica em geranato de metila

“…Entretanto, nos trabalhos reportados na literatura os rendimentos foram inferiores a 10%. [24][25][26][27][28][35][36][37] Este, portanto, é o primeiro relato da presença dessa substância em concentração tão alta. Sua elevada taxa de biossíntese pode ser normal, uma característica da espécie, ou pode ter sido provocada por fatores bióticos ou abióti-cos, circadianos ou sazonais, por exemplo.…”

Improved Analytical Method for Estimation of Essential Oil in Drug Ointment Through Rapid Static Chromatography Headspace for Quality Control Analysis

“…Gas chromatography (GC) is frequently used for the estimates these oil contents used in the preparation of different products manufactured in pharmaceutical industries [2]. As essential oils (EOs) have medicinal value and used in the pharmaceutical industries for the manufacturing of various types of ointment, these oils are natural and having more impurities, which sometimes give erratic results during drug estimations and resulted out of specification (OOS).…”