Gas Chromatography-Mass Spectrometry (GC-MS) based Metabolomics of Promising Chickpea Genotypes against Callosobruchus chinensis (L.)

Abstract

Background: The pulse beetle, Callosobruchus chinensis L. is one of the main biotic threats to chickpea in both field and storage in the world. The evaluation of chickpea genotypes for pulse beetle resistance has increased motivation for the identification and application of host plant resistance as a critical component of pest management. Methods: In the present study, different chickpea genotypes were evaluated under laboratory conditions to identify the antixenosis, antibiosis and GC-MS based untargeted metabolomics of promising chickpea genotypes to the C. chinensis. Result: Genotype NBeG1004 (36.61 eggs/100 seeds) was less prone to egg laying, while ICCV14872 (180.45 eggs/100 seeds) was the most preferred. However, maximal developmental periods, minimum per cent adult emergence and growth index was observed in ICCV92944 Genotype HC1 showed substantial resistance with maximum phenolic, flavonoid and protease inhibitors content. The results revealed that presence of 9-Octadecenoic acid (Z) in HC1 conferred resistance to C. chinensis. These results show assurance for incorporation into an IPM program against C. chinensis.

Host Plant Resistance and Analysis of Chemical Compounds Responsible for Bruchid Resistance in Greengram Vigna radiata (L.) Wilczek

“…Among the seven compounds identified four compounds viz., Hexadecanoic acid, methyl ester; n-Hexadecanoic acid; 9, 12-octadecadienoic acid (Z, Z) and Octadecaenoic acid were observed in all the four lines including susceptible check with some difference in per cent peak area. The compounds viz., Hexadecanoic acid, methyl ester; n-Hexadecanoic acid and 9, 12-octadecadienoic acid (Z,Z) were reported while studying bruchid resistant and susceptible lines in chickpea (Reddy et al, 2021) and in blackgram (Ragul et al, 2022) through GC-MS analysis. Bharathithasan et al (2021) also reported insecticidal property of Octadecaenoic acid against insect pest of Areca nut.…”

“…2). The compound 9, 12-Octadecadienoic acid, methyl ester was reported against bruchid infestation in chickpea (Reddy et al, 2021). Hexadecanoic acid, 1-(hydroxymethyl)-1, 2-ethanediyl ester and Hexadecanoic acid, 2-oxiranyl methyl ester were known to have insecticidal properties against insect pest of Areca nut (Bharathithasan et al, 2021).…”

“…The bruchid infestation begins in the field, when adult beetles lay eggs on green pods and larva bore through the pod and feed on the growing seed, causing 1-2 per cent of the damage at field level. When the seeds are stored, the insects continue to feed, mature into adults, generate a secondary infestation and resulting in the complete destruction of the seeds in 3-4 months (Reddy et al, 2021).…”

Growth Performance and Metabolic Changes in Susceptible Mung Bean [Vigna radiata (L.) Wilczek] during Interaction with Rhizoctonia solani and Trichoderma virens

“…In contrast, pathogen infection reduced several compounds such as myo-inositol, acetic acid, D-manitol, D-ribose and D-galactopyranoside. Those accumulated metabolites are pathogenesis-related (PR) metabolites which are produced et al, 2021) and some responsible for resistance against pests (Reddy et al, 2021). Higher intensities of fatty acids such as ethanedioic and stearic acids were observed in all treated plants.…”