Gas chromatography-mass spectrometry metabolomics-based prediction of potato tuber sprouting during long-term storage

Fukuda, Teruyo; Takamatsu, Kiyofumi; Bamba, Takeshi; Fukusaki, Eiichiro

doi:10.1016/j.jbiosc.2019.01.016

Cited by 12 publications

(10 citation statements)

References 26 publications

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“…These results are consistent with our models as well as with the literature since authors conducted studies on different varieties and showed that high temperatures during the growing season lead to reduced dormancy (Levy and Veilleux, 2007;Magdalena and Dariusz, 2018;Zommick et al, 2014). This impact of temperatures during the growing season on the dormancy length can be explained because high temperatures during the growing season are known to accelerate the physiological aging of the tubers (Caldiz et al, 2001) leading to several physiological and biochemical modifications related to the end of dormancy (Delaplace et al, 2009;Fukuda et al, 2019).…”

Section: Discussionsupporting

confidence: 93%

Prediction of potato sprouting during storage

Visse-Mansiaux

Soyeurt

Herrera

et al. 2022

Field Crops Research

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Section: Discussionsupporting

confidence: 93%

Prediction of potato sprouting during storage

Visse-Mansiaux

Soyeurt

Herrera

et al. 2022

Field Crops Research

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“…A range of distinct metabolites was detected that could be characterized mainly as sugars, sugar alcohols, amino acids, and organic acids (Table S2). The accumulation of these metabolites during various storage temperatures from different potato varieties have been previously reported 16,24,25 . Furthermore, PLS-DA analysis showed a divergent separation on the scores plot of PC1 and PC2, accounting for a 48.1% and 28.5% variation in the metabolites extracted from the FH and CS treated tubers of the different cultivars used in the study (Fig.…”

Section: Resultsmentioning

confidence: 90%

Cold storage reveals distinct metabolic perturbations in processing and non-processing cultivars of potato (Solanum tuberosum L.)

Datir

Yousf

Sharma

et al. 2020

Sci Rep

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Cold-induced sweetening (CIS) causes considerable losses to the potato processing industry wherein the selection of potato genotypes using biochemical information has found to be advantageous. Here, 1 H NMR spectroscopy was performed to identify metabolic perturbations from tubers of five potato cultivars (Atlantic, Frito Lay-1533, Kufri Jyoti, Kufri Pukhraj, and PU1) differing in their CIS ability and processing characteristics at harvest and after cold storage (4 °C). Thirty-nine water-soluble metabolites were detected wherein significantly affected metabolites after cold storage were categorized into sugars, sugar alcohols, amino acids, and organic acids. Multivariate statistical analysis indicated significant differences in the metabolic profiles among the potato cultivars. Pathway enrichment analysis revealed that carbohydrates, amino acids, and organic acids are the key players in CIS. Interestingly, one of the processing cultivars, FL-1533, exhibited a unique combination of metabolites represented by low levels of glucose, fructose, and asparagine accompanied by high citrate levels. Conversely, non-processing cultivars (Kufri Pukhraj and Kufri Jyoti) showed elevated glucose, fructose, and malate levels. Our results indicate that metabolites such as glucose, fructose, sucrose, asparagine, glutamine, citrate, malate, proline, 4-aminobutyrate can be potentially utilized for the prediction, selection, and development of potato cultivars for long-term storage, nutritional, as well as processing attributes. Potato (Solanum tuberosum L.)-an important staple non-grain vegetable food crop-is used globally for both processing and table purposes. In order to ensure the year-round supply of quality tubers for consumption, cold storage of potato tubers after harvesting is mandatory to reduce sprouting, prevent diseases, avoid losses due to shrinkage, and extend post-harvest shelf life 1,2. Usually 8-10 °C is the recommended storage temperature for potato tubers along with sprout suppressants, such as isopropyl N-(3-chlorophenyl) carbamate. The use of such chemical suppressants-due to their environmental safety and toxicity issues-are being replaced and reliance on storage of potatoes at colder temperatures even below 4 °C has been increased gradually 3-5. During cold storage, potato tubers undergo cold-induced sweetening (CIS), wherein rapid degradation of starch and sucrose hydrolysis leads to the accumulation of reducing sugars (RS)-such as glucose and fructose 6. During the frying process, these RS react with free amino acids in a Maillard reaction to generate dark-pigmented products that are bitter and unsightly to consumers. In addition to this, one of the products of the Maillard reaction is acrylamide-a potent neurotoxin and carcinogen 4,7. Hence, CIS is considered as one of the critical parameters in potato production as well as in processing; therefore, the identification and development of potato tubers

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“…Metabolomics combined with ionomics detected 2656 compounds in potato tubers including bioactives, nutrients, lipids and metals, nevertheless, only 184 out of them were identified (Chaparro et al, 2018 ). Fukuda et al ( 2019 ) demonstrated that GC–MS could be used even for metabolomics-based prediction of potato tuber sprouting during long-term storage. Metabolomics enabled identifying biomarkers of tuber black dot-causing Colletotrichum coccodes infection by detecting constitutive and induced resistance-related metabolites (Massana-Codina et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Metabolite profiling of tubers of an early- and a late-maturing potato line and their grafts

2022

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Introduction Earliness of tuberisation and the quality of potato tubers are important traits in potato breeding. The qualitative traits rely on the metabolite profile of tubers, which are storage organs and net importers of assimilates. Thus, the quality of tubers largely depends on the metabolites transported from leaves to developing tubers. Objectives To test the influence of canopy on the quality of tubers by metabolite profiling of tubers of an early- and a late-maturing potato line and their grafts. Methods Potatoes were grown under greenhouse conditions, grafted and the tubers harvested at the end of the scions’ vegetation period. Metabolite profiling of freshly harvested tubers was performed using gas chromatography coupled with mass spectrometry. Statistical analyses were applied to determine the significant differences between the different tubers. Results 99 metabolites were identified and an additional 181 peaks detected in chromatograms, out of which 186 were polar and 94 non-polar compounds. The concentrations of 113 metabolites were significantly different in the tubers from the early-maturing CE3130 and the late-maturing CE3027 line. Hetero-grafting resulted in considerable changes in the metabolite content of tubers. Especially, the effect of CE3027 on the metabolite composition of tubers formed on CE3130 rootstocks was readily apparent. Nevertheless, many compounds were present at similar levels in the tubers of hetero-grafted plants as was found in the tubers of their scion counterparts. Conclusion Hetero-grafting resulted in many compounds at similar concentrations in rootstock tubers as in scion tubers suggesting that these are transported from the source leaves to tubers.

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Gas chromatography-mass spectrometry metabolomics-based prediction of potato tuber sprouting during long-term storage

Cited by 12 publications

References 26 publications

Prediction of potato sprouting during storage

Prediction of potato sprouting during storage

Cold storage reveals distinct metabolic perturbations in processing and non-processing cultivars of potato (Solanum tuberosum L.)

Metabolite profiling of tubers of an early- and a late-maturing potato line and their grafts

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