Gas chromatography-mass spectrometry of isobutyl ester trimethylsilyl ether derivatives of bile acids and application to the study of bile sterol and bile acid biosynthesis in rat liver epithelial cell lines

Tsaconas, Christos; Padieu, P; Maume, G.; Chessebeuf, M; Hussein, Nadia; Pitoizet, Nicole

doi:10.1016/0003-2697(86)90631-7

Cited by 14 publications

(3 citation statements)

References 32 publications

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“…GC-MS was employed to study the inborn errors of bile acid metabolism [24]. Besides, different types of MS, such as FAB-MS [25], CI-MS [26], EI-MS, and electrospray-MS [27] have been employed routinely for the determination of bile acids. Bile acids were often analyzed by GC-MS as methyl ester-trimethylsilyl ether derivatives because of their good resolution and stability on different capillary columns.…”

Section: Introductionmentioning

confidence: 99%

Determination of bile acids in pig liver, pig kidney and bovine liver by gas chromatography-chemical ionization tandem mass spectrometry with total ion chromatograms and extraction ion chromatograms

Tsai

Zhong

Weng

et al. 2011

Journal of Chromatography A

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Section: Introductionmentioning

confidence: 99%

Determination of bile acids in pig liver, pig kidney and bovine liver by gas chromatography-chemical ionization tandem mass spectrometry with total ion chromatograms and extraction ion chromatograms

Tsai

Zhong

Weng

et al. 2011

Journal of Chromatography A

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“…Thus, the GLC retention time of the TMS ether of cholesterol is close to that of the methyl ester of lithocholic acid on a CP-Sil-5 CB capillary column, while the plant sterols, campesterol and sitosterol, which are usually present in the stool, are eluted in the general bile acid region (14). Because the retention times of bile acids increase when their esters with higher homologs are used instead of the methyl ester-trimethylsilyl ethers (19), Tsaconas et al (20) prepared the isobutyl ester-TMS ethers of bile acids and found that most common bile acids were well resolved from sterols on capillary GLC. However, we found that although the isobutyl ester-TMS ethers of bile acids were eluted later than the TMS ether of cholesterol on a CP-Sil-5 CB capillary column, the plant sterol, sitosterol, a major fecal sterol, was eluted later than lithocholic Fig.…”

Section: Discussionmentioning

confidence: 99%

Highly simplified method for gas-liquid chromatographic quantitation of bile acids and sterols in human stool

Batta¹,

Salen

Rapole³

et al. 1999

Journal of Lipid Research

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A simple method for the gas-liquid chromatographic quantitation of human fecal bile acids and sterols is described where bile acids are subjected to n-butyl ester derivatization, without prior isolation from the stool, followed by trimethylsilylation of the sterols and bile acids. Under these conditions, bile acid derivatives are well resolved from each other and from the trimethylsilyl ether derivatives of fecal sterols and no overlap occurs. The method was shown to be highly reproducible and recoveries were similar to those obtained with other methods used for fecal bile acid analysis. Application of the method for bile acid and sterol analysis in human stool is described. -Batta, A

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“…The were identified and assayed by gas-chromatographymass spectometry (GS-MS) according to Padieu et al (1985) and Tsaconas et al (1986).…”

Section: Secretion Of Bile Acids and Bile Saltsmentioning

confidence: 99%

Maintenance of liver function in long term culture of hepatocytes following In Vitro or In Vivo Ha-ras EJ transfection

et al. 1991

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Collagenase isolated rat hepatocytes were transfected with liposome encapsulated pEJ (LE-pEJ), a plasmid carrying the human cellular activated Ha-rasEJ oncogene. A proliferative cell line was cloned from these cells transfected in vitro. It secreted per day 0.87 micrograms albumin and 0.32 microgram transferrin per 10(6) cells, and 11.06 nmol free and conjugated bile acids (BA) per mg protein. Also, it metabolized 2-acetylaminofluorene (2-AAF) into N- and ring-hydroxylated metabolites and 2-aminofluorene at rates of 1.50, 9.73, and 1.98 nmol/mg cell protein/24 hr, respectively. Rats were i.v. injected with both LE-pEJ and LE-p17hGHneo carrying the hGH cDNA gene, and secreted hGH in the plasma which induced the synthesis of anti-hGH antibodies. A cell line was cloned from cultures of primary hepatocytes isolated from the liver of transfected rats. After 2 to 3 months in culture, this cell line secreted per day 18.9 micrograms albumin and 11.0 micrograms transferrin per 10(6) cells, 38.75 nmol total BA per mg cell protein, and up to 31 ng hGH per 10(6) cells without cloning hGH recombinant cells. A 24 hr control culture of primary hepatocytes isolated from non transfected rats secreted 25.5 micrograms albumin and 11.7 micrograms transferrin per 10(6) cells, and produced 21.64 nmol total BA and 2.13 nmol N-OH-2-AAF per mg cell protein. Hence, Ha-rasEJ transfection of either hepatocytes in vitro or liver cells in vivo, initiated cell cycles leading to presumptive proliferating hepatocytes which express liver function.

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Gas chromatography-mass spectrometry of isobutyl ester trimethylsilyl ether derivatives of bile acids and application to the study of bile sterol and bile acid biosynthesis in rat liver epithelial cell lines

Cited by 14 publications

References 32 publications

Determination of bile acids in pig liver, pig kidney and bovine liver by gas chromatography-chemical ionization tandem mass spectrometry with total ion chromatograms and extraction ion chromatograms

Determination of bile acids in pig liver, pig kidney and bovine liver by gas chromatography-chemical ionization tandem mass spectrometry with total ion chromatograms and extraction ion chromatograms

Highly simplified method for gas-liquid chromatographic quantitation of bile acids and sterols in human stool

Maintenance of liver function in long term culture of hepatocytes following In Vitro or In Vivo Ha-ras EJ transfection

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