Gas chromatography‐mass spectrometry of nortriptyline in body fluids of man

Abstract

The application of the mass fragmentographic technique has aUowed accurate identification of a tricyclic antidepressant drug, nortriptyline, and some of its metabolites in biological fluids from patients treated with therapeutic doses and from one patient intoxicated with the same drug. Mass fragmentography in combination with the scanning of mass spectra of compoundy eluted from the gas chromatography column made it possible to identify nortriptyline and the metabolites, desmethylnortriptyline, lO-hydroxynortriptyline, and, tentatively, lO-hydroxydesmethylnortriptyline.

Electron beam ionization mass fragmentographic analysis of tricyclic antidepressants in human plasma

“…By using mass fragmentography, N-trifluoroacetylnortriptyline was measured in cerebrospinal fluid, plasma, and urine (16). The metabolism of nortriptyline was studied in humans using nortriptyline and deuterium-labeled nortriptyline (17).…”

Clinical pharmacology in Stockholm 50 years—report from the jubilee symposium

“…They pointed out that drug analysis has long traditions at KI and mentioned in particular the publications in 1968 by Bo Holmstedt and collaborators [ 18 ], some later becoming involved in clinical pharmacology. These publications concerned massfragmentography for measuring for the first time very low blood levels of chlorpromazine and tricyclic antidepressants [ 19 ]. This technique permits a coupling interface between gas chromatography and mass spectrometry.…”

Analysis of Tricyclic Antidepressants in Human Plasma by GLC–Chemical-Ionization Mass Spectrometry with Selected Ion Monitoring

“…Previous approaches include TLC (7-lo), photometry (11)(12)(13)(14)(15)(16), radioisotopic derivatization (17,18), GLC (9,13,[19][20][21][22][23][24][25][26][27][28], and GLC--mass spectrometry with selected ion monitoring (4,(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39), which is the most accurate and among the most sensitive. To date, assays with this method have utilized electron-impact ionization techniques.…”