Gas chromatography of 3H- and 14C-labelled compounds

Matucha, M.; Smolková, Eva

doi:10.1016/s0021-9673(00)80459-8

Cited by 23 publications

(3 citation statements)

References 327 publications

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“…The isotopic effect is considerably different for deuterium than for carbon isotopes ( 12 C, 13 C, 14 C) 25. 32 To date, only partial separation has been reported for carbon isotope‐containing compounds in GLC 33. We observed a minor separation between [ 12 C 16 ]methyl hexadecanoate and [ 13 C 16 ]methyl hexadecanoate in both dimensions (Figure 2 and Figure S3).…”

Section: Resultsmentioning

confidence: 76%

Biosynthetic Studies of the Male Marking Pheromone in Bumblebees by Using Labelled Fatty Acids and Two‐Dimensional Gas Chromatography with Mass Detection

et al. 2015

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Two‐dimensional comprehensive gas chromatography (GC×GC) coupled with mass detection was used as a tool for biosynthetic studies of bumblebee pheromones. Prior to biosynthetic experiments, the chromatographic behaviour of isotopically modified esters in the GC×GC system as well as their behaviour in mass detection was studied. The male marking pheromones of Bombus lucorum, Bombus lapidarius and Bombus terrestris were investigated. Main pheromonal components are ethyl tetradec‐9‐enoate (53 %) and ethyl dodecanoate (6 %) in B. lucorum, hexadec‐9‐en‐1‐ol (52 %) and hexadecan‐1‐ol (31 %) in B. lapidarius, and 2,3‐dihydrofarnesol (58 %) and ethyl dodecanoate (15 %) in B. terrestris. The research strategy was based on 1) in vivo incubation of isotopically (2H, 13C) modified fatty acids (FAs) and analysis of their metabolites and 2) feeding experiments with 2H‐ and 13C‐labelled FAs mixed with food. It was observed that labelled FAs were modified into the most abundant aliphatic compounds present in labial gland secretions. In feeding experiments, the labelled FAs were transformed into pheromone components. Transport of the FA precursors from the fat body through haemolymph was confirmed. The results show that FAs, stored in the form of triacylglycerols in the fat body, are likely to participate in the biosynthesis of some aliphatic pheromone components.

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Section: Resultsmentioning

confidence: 76%

Biosynthetic Studies of the Male Marking Pheromone in Bumblebees by Using Labelled Fatty Acids and Two‐Dimensional Gas Chromatography with Mass Detection

et al. 2015

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“…In biochemistry and pharmacology it is often possible to employ radiolabeled precursors when characterizing unknown metabolites. Gas chromatography with detection of radioactivity has long utilized nuclear decay to permit very specific compound localization in clomplex chromatograms (1,2). The design of combustion chiambers and radiochemical detectors significantly degrades the chromatographic resolution attainable with capillary columns (3-5).…”

Section: Capillary Gas Chromatography/mass Spectrometry With a Microwmentioning

confidence: 99%

“…LITERATURE CITED (1) Arpiño, P. J.; Krien, P. Proceedings of the 26th Annual Conference on Mass Spectrometry and Allied Topics, St. Louis, MO, 1978; pp 426-428. (2) Arpiño, P. J.; Guiochon, G.; Krien, P.; Devant, G. J.…”

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confidence: 99%

Capillary gas chromatography/mass spectrometry with a microwave discharge interface for determination of radioactive carbon-containing compounds

Markey¹,

Abramson²

1982

Anal. Chem.

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Radio‐chromatography. The chromatography and electrophoresis of radiolabelled compounds. T.R. Roberts. $39.95

Cacace¹

1981

Labelled Comp Radiopharmac

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Gas chromatography of 3H- and 14C-labelled compounds

Cited by 23 publications

References 327 publications

Biosynthetic Studies of the Male Marking Pheromone in Bumblebees by Using Labelled Fatty Acids and Two‐Dimensional Gas Chromatography with Mass Detection

Biosynthetic Studies of the Male Marking Pheromone in Bumblebees by Using Labelled Fatty Acids and Two‐Dimensional Gas Chromatography with Mass Detection

Capillary gas chromatography/mass spectrometry with a microwave discharge interface for determination of radioactive carbon-containing compounds

Radio‐chromatography. The chromatography and electrophoresis of radiolabelled compounds. T.R. Roberts. $39.95

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