Gas chromatography of fatty acids

Shantha, N.C.; Napolitano, Guillermo E.

doi:10.1016/0021-9673(92)85673-h

Cited by 144 publications

(77 citation statements)

References 66 publications

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“…It is therefore necessary to optimize the derivatization procedure in order to obtain accurate, quantitative and qualitative results while addressing potential procedural difficulties associated with ester preparation. 44 Common problems include:…”

Section: Introductionmentioning

confidence: 99%

Derivatization of fatty acids and its application for conjugated linoleic acid studies in ruminant meat lipids

et al. 2005

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Conjugated linoleic acid (c9, t11 CLA) is a dietary fatty acid produced mainly by ruminant animals and exhibits promising health-promoting biological effects. For lipid fatty acid composition analyses, including CLA, lipids must be pre-treated so that the free and esterified fatty acids (triacylglycerols, phospholipids, etc) are available for determination. The most common treatments involve fatty acid methyl ester derivatives from relatively simple chemical reactions, but this becomes complicated when esterification of CLA is involved because of potential changes in its positional and geometrical isomers by reaction with certain reagents. In this review we explain concisely the advantages and disadvantages of the most popular methods (acid-and base-catalysed methods) generally employed for total fatty acid derivatization and their determination on a gas chromatograph. Based on our experiences we put forward the (trimethylsilyl)diazomethane method as an alternative and successful approach for ruminant tissue lipid determinations.

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Section: Introductionmentioning

confidence: 99%

Derivatization of fatty acids and its application for conjugated linoleic acid studies in ruminant meat lipids

et al. 2005

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“…Fatty acids were identified and their concentrations were determined in fat extracted from feed. After the methylation of fatty acids in an acidic environment (Peisker 1964), fatty acid methyl esters were separated using a PYE Unicam 104 gas chromatograph (Shantha and Napolitano 1992) equipped with a flame ionization detector (FID). The results were recorded using a Philips recorder with a tape speed of 0.3 m/h.…”

Section: Methodsmentioning

confidence: 99%

The effect of diets supplemented with fish broth and fish oil on the health of weaners

Bakuła¹,

Lis²,

Iwaniuk³

et al. 2011

Polish Journal of Veterinary Sciences

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The aim of this study was to determine the effect of fish-based feed materials, as a source of readily available protein contained in fish broth and essential polyunsaturated fatty acids (PUFAs) found in fish oil, on the health of piglets and rearing results. The experiment was conducted on a commercial pig fattening farm. The study involved a total of 80 weaners with an approximate body weight of 15 kg. The experiment was carried out over a period of 40 days. Feed samples were subjected to laboratory analyses. Blood samples were collected from experimental group animals to determine serum biochemical and immunological parameters. The body weight gains of weaners, mortality rates and average feed intake per animal were calculated for the entire experimental period. The addition of fish broth and fish oil significantly improved the n3:n6 fatty acid ratio in diets. The presence of EPA and DHA in the experimental diet could have had a positive health effect on piglets, comparable with that exerted by therapeutic doses of zinc often administered to pigs of this age group. During the experiment, feed conversion ratio (FCR) gain was considerably reduced in the experimental group, with similar daily gains in the control and experimental group.

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“…Of notable interest is the use of 14% boron trifluoride/methanol which is widely used in the transmethylation of fatty acids in red blood cells [1,17,27,28]. Other methylating reagents used include acetyl chloride [29], 2% sulfuric acid in methanol [30] and sodium methoxide [16,31].…”

Section: Methylation Proceduresmentioning

confidence: 99%

A Rapid and Simple Method for Fatty Acid Profiling and Determination of ω-3 Index in Red Blood Cells

Akinyemi¹,

Bruckner²,

Johnson³

et al. 2017

TONUTRJ

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Fatty acid profiling has become a very useful and effective tool in the diagnosis, prevention and treatment of several diseases with cardiovascular disease being particularly important. In order to arrive at accurate conclusions that would help promote the health of individuals plagued by such diseases, not only excellent laboratory methods are required, but also very important monitoring responses to treatment. Improvements in methods of fatty acid profiling in biological systems regarding safety of extraction, precision and time for analysis are valuable. The ω-3 index (a measure of the amount of eicosapentaenoic acid, EPA, and docosahexaenoic acid, DHA, in Red Blood Cell membranes expressed as the percent of total fatty acids) is of growing interest because it has been reported to provide prognostic information regarding the risk for heart diseases. Sodium methoxide has been widely used for the determination of ω -3 fatty acids in food samples. This study demonstrates that sodium methoxide can be used effectively in RBC fatty acid profiling and determination of the ω-3 index. Briefly, the fatty acid profiles and ω-3 index of red blood cell samples were analyzed and results compared using three different methods: a two-step extraction and methylation method described by Hara and Radin, a single step extraction and methylation method described by Harris et al. and the sodium methoxide method.Our results revealed that there were no statistically significant differences (p<0.05) between the three methods for the representative fatty acids, [16:0 (p = 0.10), 18:0 (p=0.40), 18:1(ω9) (p = 0.29), 18:2(ω6) (p = 0.95), 18:3(ω3) (p = 0.50), 20:5(ω3) (p=0.56), 22:6(ω3) (p = 0.06)] and ω-3 index (p = 0.11) except for 20:4(ω6), (P = 0.02). In conclusion, we show that sodium methoxide can be used effectively in a one-step extraction and methylation procedure for high throughput analysis of fatty acids in red blood cell membranes. It is rapid (10 minute extraction and methylation), simple, safer than and as accurate as other commonly reported methods.

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Gas chromatography of fatty acids

Cited by 144 publications

References 66 publications

Derivatization of fatty acids and its application for conjugated linoleic acid studies in ruminant meat lipids

Derivatization of fatty acids and its application for conjugated linoleic acid studies in ruminant meat lipids

The effect of diets supplemented with fish broth and fish oil on the health of weaners

A Rapid and Simple Method for Fatty Acid Profiling and Determination of ω-3 Index in Red Blood Cells

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