Gas chromatography–vacuum ultraviolet spectroscopy for analysis of fatty acid methyl esters

Fan, Haibo; Smuts, Jonathan; Bai, Ling; Walsh, Philip; Armstrong, Daniel W.; Schug, Kevin A.

doi:10.1016/j.foodchem.2015.08.004

Cited by 71 publications

(35 citation statements)

References 27 publications

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“…Compared to solution phase absorption analysis, VUV absorption spectra recorded in the gas phase display less spectral broadening due to a lack of interaction between the molecule and solvent . Gas‐phase VUV (and UV) absorption spectra show more features than those observed in solution phase absorption analysis, and spectral features are highly reflective of minor variations in the structure of analytes.…”

Section: Resultsmentioning

confidence: 99%

Analysis of terpenes and turpentines using gas chromatography with vacuum ultraviolet detection

Qiu

Smuts

Schug

2017

J of Separation Science

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The separation and identification of natural mixtures of terpenes is challenging and laborious. A gas chromatographic method based on vacuum ultraviolet spectroscopic detection, which is characterized by full-scan absorption in the range of 125-240 nm, was developed and applied to analyze terpenes. In this study, the vacuum ultraviolet absorption spectra of 41 different standard terpenes were investigated and compared. The spectra were found to be highly featured and easily differentiated. Several commercial turpentine samples were analyzed and the vacuum ultraviolet detector demonstrated good specificity for qualitative identification of constituent terpenes. A total of 31 terpenes were detected in the four turpentine samples. α-Pinene was the predominant terpene ranging from 744.2 ± 9.7 to 917 ± 21 mg/mL. The other major constituents in the turpentines included β-pinene, δ-3-carene, camphene, and p-isopropyltoluene. Deconvolution of co-eluting signals of terpenes was achieved utilizing the data analysis software. The technique has been demonstrated to be a powerful tool for reliable and accurate qualitative and quantitative analysis of terpenes from complex natural mixtures.

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Section: Resultsmentioning

confidence: 99%

Analysis of terpenes and turpentines using gas chromatography with vacuum ultraviolet detection

Qiu

Smuts

Schug

2017

J of Separation Science

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“…In addition to proper sample preparation, selection of appropriate analytical tool is paramount for fatty acid analysis. Wide range of analytical tools, including GC [ 173 , 174 , 176 , 179 , 182 ], GC-MS, gas-chromatography vacuum ultraviolet spectroscopy (GC-Vacuum UV) [ 178 ], gas-chromatography coupled with flame ionization detection (GC-FID) [ 170 , 172 ], liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS) [ 171 , 183 ], UHPLC-MS/MS [ 142 ], Raman spectroscopy [ 181 ], FTIR [ 188 , 189 ] and many more improved platforms have been used to perform FA analysis. Fatty acid analysis in LC-MS instrument showed some drawbacks in term of larger solvent consumption and lower selectivity [ 190 ].…”

Section: Fatty Acids and Cholesterolmentioning

confidence: 99%

“…While Liu et al [ 165 ] combined GC-MS and LC-MS to analyze fatty acids in milk. Application of vacuum ultraviolet (VUV) detector in GC offers additional resolution and advantage of differentiating cis/trans isomers with niche selectivity for FAME analysis [ 178 ].…”

Section: Fatty Acids and Cholesterolmentioning

confidence: 99%

Recent Techniques in Nutrient Analysis for Food Composition Database

et al. 2020

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Food composition database (FCD) provides the nutritional composition of foods. Reliable and up-to date FCD is important in many aspects of nutrition, dietetics, health, food science, biodiversity, plant breeding, food industry, trade and food regulation. FCD has been used extensively in nutrition labelling, nutritional analysis, research, regulation, national food and nutrition policy. The choice of method for the analysis of samples for FCD often depends on detection capability, along with ease of use, speed of analysis and low cost. Sample preparation is the most critical stage in analytical method development. Samples can be prepared using numerous techniques; however it should be applicable for a wide range of analytes and sample matrices. There are quite a number of significant improvements on sample preparation techniques in various food matrices for specific analytes highlighted in the literatures. Improvements on the technology used for the analysis of samples by specific instrumentation could provide an alternative to the analyst to choose for their laboratory requirement. This review provides the reader with an overview of recent techniques that can be used for sample preparation and instrumentation for food analysis which can provide wide options to the analysts in providing data to their FCD.

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“…In a similar fashion, in the work by Fan et al. , the VUV detector was used to distinguish cis ‐/ trans ‐fatty acid distributions in oils and fats using their distinct VUV spectra. Usually, this discrimination is performed using special stationary phases to chromatographically resolve these compounds.…”

Section: The Vacuum Uv Spectrophotometermentioning

confidence: 99%

“…An example of deconvolution is given in Fig. where an almost perfectly co‐eluting peak consisting of p,p’‐DDE and dieldrin pesticides was deconvoluted into two individual peaks .…”

Section: Data Treatmentmentioning

confidence: 99%

Recent advances and applications of gas chromatography vacuum ultraviolet spectroscopy

Santos

Schug

2016

J of Separation Science

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The vacuum ultraviolet spectrophotometer was developed recently as an alternative to existing gas chromatography detectors. This detector measures the absorption of gas-phase chemical species in the range of 120-240 nm, where all chemical compounds present unique absorption spectra. Therefore, qualitative analysis can be performed and quantification follows standard Beer-Lambert law principles. Different fields of application, such as petrochemical, food, and environmental analysis have been explored. Commonly demonstrated is the capability for facile deconvolution of co-eluting analytes. The concept of additive absorption for co-eluting analytes has also been advanced for classification and speciation of complex mixtures using a data treatment procedure termed time interval deconvolution. Furthermore, pseudo-absolute quantitation can be performed for system diagnosis, as well as potentially calibrationless quantitation. In this manuscript an overview of these features, the vacuum ultraviolet spectrophotometer instrumentation, and performance capabilities are given. A discussion of the applications of the vacuum ultraviolet detector is provided by describing and discussing the papers published thus far since 2014.

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Gas chromatography–vacuum ultraviolet spectroscopy for analysis of fatty acid methyl esters

Cited by 71 publications

References 27 publications

Analysis of terpenes and turpentines using gas chromatography with vacuum ultraviolet detection

Analysis of terpenes and turpentines using gas chromatography with vacuum ultraviolet detection

Recent Techniques in Nutrient Analysis for Food Composition Database

Recent advances and applications of gas chromatography vacuum ultraviolet spectroscopy

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