Gas-liquid chromatographic separation of C19 and C21 human urinary steroids by a new procedure

Hypoaldosteronism in three sibs due to 18-dehydrogenase deficiency.

“…The final quantitation of the metabolites of cortisol and cortisone was by the blue tetrazolium reaction using a twice purified blue tetrazolium preparation alkaline solution (Visser and Cost, 1964). The metabolites of compounds A, B, and 18-OHA were quantitated by GLC of their MO-TMSi derivatives (methyloxime-trimethylsilyl ether) after the method of Gardiner and Herning (1966) against a reliable standard of each compound injected on to the column 15 minutes after the respective test samples.…”

Virilizing adrenal tumour in a 9-year-old female

“…Urinary 17 hydroxycorticosteroid output was elevated and ranged from 120 /xmol/24 h to 164 /(mol/24 h. (Normal 3-5-21 /(mol/1 h.) Urinary 17 oxosteroid output was also grossly elevated, ranging from 169 /imol/24 h to 247 /jmol/24 h. (Normal O-9-IO-4 /imol/24 h.) There was no suppression of urinary steroid output following the administration of dexamethasone 8 mg/day. Fractionation of urinary steroids, using a modification of the gas chromatographic technique of Gardiner & Horning (1966) showed elevation of most steroids. In particular, there was a disproportionate increase in the excretion of dehydroepiandrosterone (DHA) see Eig.…”

Familial Pseudohypoaldosteronism

“…In brief, steroid profiling was done on a dual column system equipped with column switching facilities for better peak resolution. Enzymatically hydrolysed steroids were analysed as methoxime-trimethylsilyl ethers (17,18). Results were evaluated by means of an integrator interface coupling device.…”