Gas-liquid chromatography: retention volumes of the methyl esters of fatty acids with special reference to n-odd-numbered ISO and (+)-Anteiso acids

Hawke, J. C.; Hansen, R. P.; Shorland, F. B.

doi:10.1016/s0021-9673(01)86340-8

Cited by 68 publications

(18 citation statements)

References 15 publications

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“…This conclusion was supported by the fact that the mass spectrum of peak a could be distinguished clearly from those of other methylbranched pentadecanoate, such as 12-methylpentadecanoate, 11-pentadecanoate etc [1]. The retention time of peak a by gas-liquid chromatography is identical with that of iso-pentadecanoic acid methyl ester [13]. Similarly, peak d (Fig.…”

Section: Methodsmentioning

confidence: 82%

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Chemical and Immunological Studies on the Cell Walls of Propionibacterium acnes Strain C7 and Corynebacterium parvum ATCC 11829

Azuma

Sugimura

Taniyama

et al. 1975

Japanese Journal of Microbiology

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The chemical and immunological properties of the cell walls prepared from the cells of anaerobic coryneforms, Propionibacterium acnes C7 and Corynebacterium parvum ATCC 11829, were partially investigated. The cell walls prepared from P. acnes C7 and C. parvum ATCC 11829 were composed of fatty acids, polysaccharides consisting glucose, galactose and mannose and mucopeptides consisting mainly of alanine, glutamic acid, a, ε‐diaminopimelic acid, glycine, muramic acid and glucosamine. As the fatty acid constituents of the cell wall of P. acnes C7, iso‐pentadecanoic acid and iso‐heptadecanoic acid were detected as major components. Both cell walls prepared from P. acnes C7 and C. parvum ATCC 11829 showed potent adjuvant activity on the formation of circulating antibody and development of delayed type hypersensitivity in vivo and on the primary immune response to sheep erythrocytes in vitro, however, could not augment helper function of carrier‐primed T cells and on the development of cell‐mediated cytotoxicity to mastocytoma P815‐X2 cells in C57BL/6J mice. It is also shown that the cell walls of P. acnes C7 and C. parvum ATCC 11829 act on mouse spleen cells as mitogen.

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Section: Methodsmentioning

confidence: 82%

“…1). The peaks b,c and e were identified as the methyl esters of n-pentadecanoic acid, palmitic acid, and n-heptadecanoic acid respectively by the retention times of gas-liquid chromatographic [13] and mass spectrometric [1] analysis by using authentic materials. The mass spectrum of peak a (Fig.…”

Section: Methodsmentioning

confidence: 99%

Chemical and Immunological Studies on the Cell Walls of Propionibacterium acnes Strain C7 and Corynebacterium parvum ATCC 11829

Azuma

Sugimura

Taniyama

et al. 1975

Japanese Journal of Microbiology

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“…The preparation of the columns and the operating parameters for the instrument have been described in detail by Vorbeck et al (1961). The techniques described by James (1959) and Hawke et al (1959), using the relative retention volume on a polar and nonpolar stationary phase and establishing a grid using known acids, were employed to identify the fatty acids. The acids were quantitated by peak-area integration.…”

Section: Lipid Fractionationmentioning

confidence: 99%

The Effect of Boron on the Lipid Content and Discoloration of Potatoes

Mondy¹,

Bourque²,

Breslow³

et al. 1965

Journal of Food Science

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SUMMARY Ontario, Katahdin, and Pontiac potatoes grown with and without boron foliar spray were examined for discoloration and lipid content. The lipid was fractionated into free fatty acids, neutral fat, and phospholipids. The free fatty acids from each of the three fractions were esterified and determined quantitatively by gas chromatography. Discoloration was measured with a Hunter color‐difference meter. The total lipid of all three varieties was higher in potatoes treated with boron than in the control potatoes. Cortex tissue was significantly higher in lipid content that center tissue. Pontiac potatoes, the variety most resistant to discoloration, had the highest lipid content. The phospholipid fraction of all three varieties was higher in the potatoes treated with boron, and the neutral‐fat fraction was higher in Pontiac potatoes treated with boron than in untreated potatoes. Treatment of potatoes with boron tended to increase the amount of unsaturated and decrease the amount of saturated fatty acids. The free fatty acid fractions of potatoes receiving boron treatment were lower in palmitic and higher in linoleic acid than the control, the neutral fat fraction was higher in linolenic acid, and the phospholipid fraction was lower in palmitic acid. Potatoes receiving boron discolored less than control potatoes.

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“…(Table I), but it is feasible t o group these acids in possible arrangements based on literature values (9,18) for the separation of iso, anteiso, and neo acids from the straight chain fatty acids with the same number of carbon atoms (Tables VI and VII). The final identification of the structures depends on determination of number of carbon atoms and on physicochemical (19) or chemical (20) examination, but the order of appearance in the urea separation and the groupings by separation from potential normal acids suggest t h a t B, D, and F are respectively CI5, C16, and C17 is0 acids; C and Y respectively C16 and C16 anteiso acids, although C16 and C17 neo acids cannot be entirely excluded as possibilities;…”

Section: Saturated Branch Chain Acidsmentioning

confidence: 99%

“…------ Odd-nz~mbercd Fatty A cids These acids may originate from microbiological activity (21) but are now accepted a s normal components of shark liver (18,22) and menhaden oils (9,23). In the seal oil there is clear evidence for the saturated C16, C17, and C19 acids, but not for the C21.…”

Section: Saturated Branch Chain Acidsmentioning

confidence: 99%

Systematic Identification of Fatty Acids in the Gas–liquid Chromatography of Fatty Acid Methyl Esters: A Preliminary Survey of Seal Oil

Ackman¹,

Burgher²,

Jangaard³

1963

Can. J. Biochem. Physiol.

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The fatty acids of a commercial seal blubber oil have been surveyed by application of the linear log plot and separation factor procedures to the gas-liquid chromatograins of methyl esters obtained from urea complexing. These techniques, coupled with the use of two polyesters, have permitted the tentative identification of virtually all of the components. The overall composition of the seal blubber oil appears to cover the same range of fatty acids as the body oils of inenhaden and herring.

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Gas-liquid chromatography: retention volumes of the methyl esters of fatty acids with special reference to n-odd-numbered ISO and (+)-Anteiso acids

Cited by 68 publications

References 15 publications

Chemical and Immunological Studies on the Cell Walls of Propionibacterium acnes Strain C7 and Corynebacterium parvum ATCC 11829

Chemical and Immunological Studies on the Cell Walls of Propionibacterium acnes Strain C7 and Corynebacterium parvum ATCC 11829

The Effect of Boron on the Lipid Content and Discoloration of Potatoes

Systematic Identification of Fatty Acids in the Gas–liquid Chromatography of Fatty Acid Methyl Esters: A Preliminary Survey of Seal Oil

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