Gas phase silylation, a rapid method for preparation of high-performance liquid chromatography supports

Wikström, Pernilla; Mandenius, Carl Fredrik; Larsson, Per‐Olof

doi:10.1016/s0021-9673(01)82110-5

Cited by 49 publications

(28 citation statements)

References 14 publications

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“…We found that vapor depositions of short chain amine-terminated alkylsilanes could be accomplished with relatively mild vacuum (125-150 Torr) and at room temperature to produce thin-films of sufficient quality for our detection platform (see assay results below). This method avoided the use of complex instrumentation, high temperature, or high pressure or high vacuum methods to create our desired films [27,[35][36][37][38][39][40][41][42]. Water contact angles for vapor-deposited APMDES SAMs ranged from 60-65°, which was consistent with previous reports for APMDES SAMs [33].…”

Section: Preparation Of Functional Silane Filmssupporting

confidence: 87%

Robust sensing films for pathogen detection and medical diagnostics

et al. 2009

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Our team has developed polyethylene glycol (PEG)-modified, self-assembled monolayers (SAMs) for biological detection on either planar or spherical substrates, which resist non-specific binding while facilitating specific ligand attachment. The preparation and characterization of these thin films, their validation against B. anthracis protective antigen (PA) in a sandwich assay format, and the application of these thin films for quantitative analysis of several medically interesting targets (breast cancer, tuberculosis, and influenza) will be shown.

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Section: Preparation Of Functional Silane Filmssupporting

confidence: 87%

Robust sensing films for pathogen detection and medical diagnostics

et al. 2009

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“…Silane films were prepared by methods described elsewhere with slight modifications (Wikstrom et al ., 1988;Falsey et al ., 2001).…”

Section: Silane Film Preparationmentioning

confidence: 99%

Morphology and Amine Accessibility of (3‐Aminopropyl) Triethoxysilane Films on Glass Surfaces

Wang

Vaughn

2008

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3-Aminopropyl) triethoxysilane (APTES) is commonly used to functionalize glass substrates because it can form an amine-reactive film that is tightly attached to the surface. In this study, we investigated the morphology and chemical reactivity of APTES films prepared on glass substrates using common deposition techniques. Films were prepared using concentrated vapor-phase deposition, dilute vapor-phase deposition, anhydrous organic-phase deposition and aqueous-phase deposition. All films were annealed, or cured, at 150 degrees C. The morphology of the films was quantified by fluorescence and by atomic force microscopy (AFM). The optical equivalent of the AFM images was computed and then used to directly compare optical and AFM images. Reactive amine density was determined by a picric acid assay and by a method that employed N-succinimidyl 3-[2-pyridyldithio]-propionamido (SPDP) cross-linked rhodamine. Fluorescence and AFM images showed that silane films prepared from dilute vapor-phase and aqueous-phase deposition were more uniform and had fewer domains than those deposited by the other methods. The ratio of picric acid-accessible amino groups to SPDP cross-linked rhodamine-accessible groups varied with the preparation method, suggesting reactant size-dependent difference in amine accessibility. We found a larger number of accessible amino groups on films prepared by vapor-phase deposition than on those prepared from solution deposition. The dilute vapor-phase deposition technique produced relatively few domains, and it should be a good choice for bioconjugation applications. There were appreciable differences in the films produced by each method. We suggest that these differences originate from differences in film rearrangement during annealing.

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“…Collagen type I (rat tail)‐coated plates were prepared by covalent cross‐linking of soluble collagen (1 mg/ml) to polystyrene four‐well plates. The plastic surface was activated by incubation with 3‐aminopropyltrimethoxysilane (4%) in 0·1 mol/l sodium acetate (pH 5·5) for 3 h at 90°C (Wikstrom et al , 1988). The plates were washed and further incubated with glutaraldehyde (3%) for 18 h, rewashed and exposed to collagen (1 mg/ml in 10 mmol/l HCl) for 2 h at 22°C, followed by an extensive wash and maintenance at 4°C for up to 1 week prior to use.…”

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Transient adhesion refractoriness of circulating platelets under shear stress: the role of partial activation and microaggregate formation by suboptimal ADP concentration

et al. 2001

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Summary. Exposure of whole blood (WB) to subendothelial extracellular matrix (ECM) under shear stress in the cone and plate(let) analyser (CPA) results in platelet adhesion, followed by release reaction and aggregation of circulating platelets on the adherent platelets. The properties of circulating non-adhered platelets in the CPA was studied by exposure of WB to ECM at a high shear rate (1300/s) for 2 min (1st run), followed by transfer of the suspension to a new ECM-coated well for a second run (2nd run) under similar conditions. The results of the 2nd run demonstrated transient adhesion refractoriness associated with platelet microaggregate formation in the suspension. The adhesion refractoriness was dependent on platelet activation during the 1st run and was prevented by addition of apyrase (ADP scavenger) or ADP receptor inhibitor, suggesting a role for ADP in mediating this response. Furthermore, exposure of WB samples to suboptimal concentrations of ADP (0´4± 1 mmol/l) or a thrombin receptor activating peptide (TRAP) (5 mmol/l) for 2 min resulted in a similar transient platelet adhesion refractoriness to ECM under flow conditions. The transient platelet refractoriness and microaggregate formation induced by ADP was associated with a transient reduction in glycoprotein (GP)Ib, increased P-selectin expression and increased fibrinogen binding by circulating platelets. These data suggest a role for platelet agonists at suboptimal concentrations in modulating platelet function and limiting the expansion of the thrombus.

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Gas phase silylation, a rapid method for preparation of high-performance liquid chromatography supports

Cited by 49 publications

References 14 publications

Robust sensing films for pathogen detection and medical diagnostics

Robust sensing films for pathogen detection and medical diagnostics

Morphology and Amine Accessibility of (3‐Aminopropyl) Triethoxysilane Films on Glass Surfaces

Transient adhesion refractoriness of circulating platelets under shear stress: the role of partial activation and microaggregate formation by suboptimal ADP concentration

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