Gaseous Ozone-Treated Human Dental Pulp Stem Cells Secretome Enhances Angiogenesis in a Chick Embryo Model

Abstract

Background: Ozone has become more common in medicine across the globe as an adjuvant treatment method for a variety of illnesses. The combination of ozone and treated human dental pulp stem cell-conditioned media hDPSCs-CM can enhance angiogenesis due to their synergistic action, resulting in increased growth factor expression. Objectives: The aim of the study was to evaluate the angiogenic potential of ozone-treated human dental pulp stem cell-conditioned media (hDPSCs-CM). Materials and Method: HDPSCs were isolated from the extracted tooth. Passaged four cells were characterized with flow cytometry and then exposed to 10μg/mL gaseous Ozone concertation. The conditioned media (CM) were obtained from the treated cells, and growth factor analysis was performed. The functionality of the Ozonated hDPSCs-CM was assessed by the Chick Yolk Sac Membrane (YSM) assay. Results: Ozonated hDPSCs-CM had significantly higher (p<0.01) expression of angiopoietin-2 (Ang-2), fibroblast growth factor (FGF), hepatocyte growth factor (HGF), macrophage colony stimulating factor (M-CSF), and vascular endothelial growth factor (VEGF). The in-ovo YSM assay revealed a notably greater pro-angiogenic potential associated with Ozonated hDPSC-CM