Gases invernadero en aguas con bajo oxígeno en el reservorio eutrófico de Prado (Colombia)

González, Maribeb Castro; Torres-Valdés, Victoria

doi:10.18257/raccefyn.228

Cited by 1 publication

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References 28 publications

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“…For the reservoir, nitrate levels are related to the planktonic and benthic activities (Márquez and Guillot 2001), and the increase in nitrate level observed at Isla del Sol may be due to a higher nitrifying (to 1.2mg/L O 2 ) than denitrifying activity, to difference that observed at Lozanía where the lower O 2 level (0.01mg/L) favored the denitrification by a great variety of nosZ-OTUs. Our assessment is supported by studies showing as both ammonia and nitrite oxidation occurred at very low oxygen concentrations (even < 50 µM) in marine (Peng et al 2015) and freshwater ecosystems (Wetzel 2001, Castro-González andTorres 2015).…”

Section: Discussionsupporting

confidence: 80%

Spatial Variation of -communities in the Low Oxygen Waters of Prado Hydroelectric (South-West of Colombia)

Montealegre¹,

González²

2016

Open Ecol. J.

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Denitrification is a process of reduction of nitrate to dinitrogen by facultative anaerobic microorganisms, which have functional genes encoding denitrification enzymes (reductases). The nosZ gene encoding the enzyme that reduces nitrous oxide to dinitrogen was utilized in this research, as molecular marker for denitrifying communities into low oxygen waters of Prado reservoir. Our objective was to analyze and compare the composition (richness and abundance) of nosZ-type denitrifiers in relationship with physicochemical variables (oxygen, pH, temperature, nitrate, nitrite and ammonium) in three areas of this dam: Isla del Sol, Lozanía and Tomogó which are distant and have different anthropogenic influences. For this, we performed DNA extraction, amplification, 454 pyrosequencing and phylogenetic analysis of nosZ gene. The Chao1 estimator and Shannon index were used for compare richness and diversity of nosZ gene; and the relationship between compositions of operational taxonomic units (OTU) with physicochemical variables was established by canonical correspondence analysis (CCA). In the reservoir 45 nosZ-OTUs to species level (80% similarity) were detected. Lozanía had the highest number of OTUs (25) and greatest diversity (S CHAO1 : 35; H: 2.0), compared to the other areas sampling. The phylogenetic analysis showed the presence of many OTUs (28 of 45) with low similarity to Proteobacteria group and high similarity with sequences of environmental clones reported previously. The CCA showed that the nosZ-community composition of Prado dam was related with low pH (6.2), oxygen (0.01mg / L) and nitrate (<0.25 mg / L) recorded in the water column.

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Section: Discussionsupporting

confidence: 80%