Gastric atrial natriuretic peptide regulates endocrine secretion in antrum and fundus of human and rat stomach

Abstract

. Gastric atrial natriuretic peptide regulates endocrine secretion in antrum and fundus of human and rat stomach. Am J Physiol Gastrointest Liver Physiol 284: G638-G645, 2003; 10.1152/ajpgi. 00427.2002-Atrial natriuretic peptide (ANP) is present in gastric mucosa and preferentially binds to two subtypes of natriuretic peptide receptors (NPR), NPR-A and NPR-C. The present study examines the role of endogenous ANP in regulating endocrine secretion in rat and human stomachs. NPR-A protein expression and transcripts were identified in rat antral and fundic mucosa by Western blot and RT-PCR. In superfused rat and human antral and fundic segments, ANP (0.1 pM to 0.1 M) caused a concentration-dependent increase in somatostatin secretion. In antrum, this was accompanied by a decrease in gastrin, and in fundus, this was accompanied by a decrease in histamine secretion. Changes in gastrin and histamine secretion reflected changes in somatostatin secretion and were abolished by somatostatin antibody. The NPR-A receptor antagonist anantin 1) inhibited basal somatostatin secretion and 2) abolished the somatostatin, gastrin, and histamine responses to ANP. We conclude that endogenous ANP, acting via the NPR-A receptor, stimulates somatostatin secretion from both antrum and fundus of rat and human stomach. Stimulation of somatostatin secretion is coupled to inhibition of gastrin secretion in the antrum and inhibition of histamine secretion in the fundus.

Natriuretic peptide receptor A inhibition suppresses gastric cancer development through reactive oxygen species-mediated G2/M cell cycle arrest and cell death

“…Accumulating evidence has shown that ANP and its receptor NPRA are also synthesized and expressed locally by many other organs [38], including the digestive system [39][40][41], and they are implicated in physiological and pathological activities of the human digestive system, such as pancreatic and gastric secretion [38,42,43]. These properties remind us about the possible regulatory role of ANP-NPRA signaling in gastric cancer progression.…”

Atrial stretch increases the gastric tonus of anesthetized rats

“…*, P b 0.05 vs. basal interval after ANOVA and Bonferroni's test. released from the gastric antrum (Gower et al 2003), we also cannot rule out a paracrine component in the action of ANP on GV. However, C-type natriuretic peptide, another member of the natriuretic family, has inhibited the gastric smooth muscle contractile behavior in vitro (Guo et al 2003).…”

Aortocaval fistula delays gastric emptying of liquid test meal in awake rats

“…In addition to their vasoactive and diuretic properties, these agents alter the activity of gut smooth muscle and may enhance gastric tonus (16). Moreover, we have shown that blood volume redistribution modifies gastrointestinal motor behavior (14,40).…”