Gastric exocrine and endocrine secretion

Schubert, Mitchell L.

doi:10.1097/mog.0b013e328331b62a

Cited by 64 publications

(36 citation statements)

References 53 publications

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“…Another important feature is that the degree of indirect or direct mediation of histamine by gastrin is not altered during ACh stimulation, suggesting that under our experimental conditions ACh may have no effect on the G cell. This is different from other studies on various species where gastrin levels have been shown to be elevated or inhibited by cholingeric agents (13,34).…”

Section: Discussioncontrasting

confidence: 50%

“…In A, gastrin predominately stimulates gastric acid secretion by activating the cholecysokinin-2 (CCK2) receptors on the parietal cell rather than via the ECL cell. However, it is currently believed that gastrin stimulates the ECL cell to release histamine (34,35). When decomposing the total output of the parietal cell, gastrin plays a more significant role on gastric acid release than histamine.…”

Section: Discussionmentioning

confidence: 99%

“…We have stimulated acid secretion using ACh to enhance acid output, but importantly, to activate the release of somatostatin, which is known to inhibit acid secretion and histamine release from the ECL cell (34,35) under basal conditions. The activation of the ACh acts as a mimic to the meal ingestion state and thus can provide useful information on the alterations in acid secretion during this phase.…”

Section: Discussionmentioning

confidence: 99%

“…ACh is released from postganglionic neurons and stimulates the parietal cells directly via M3 receptors and indirectly via M2 and M4 inhibition of somatostatin secretion (34,35). Many studies have shown that ACh stimulates the ECL cells to drive acid secretion (6,29,31).…”

Section: Studies Utilizing Isolated Ecl Cells and In Vitro Assays Havementioning

confidence: 99%

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Simultaneous detection of gastric acid and histamine release to unravel the regulation of acid secretion from the guinea pig stomach

Bitziou

Patel

2012

American Journal of Physiology-Gastrointestinal and Liver Physi

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Gastric acid secretion is regulated by three primary components that activate the parietal cell: histamine, gastrin, and acetylcholine (ACh). Although much is known about these regulatory components individually, little is known on the interplay of these multiple activators and the degree of regulation they pose on the gastric acid secretion mechanism. We utilized a novel dual-sensing approach, where an iridium oxide sensor was used to monitor pH and a boron-doped diamond electrode was used for the detection of histamine from in vitro guinea pig stomach mucosal sections. Under basal conditions, gastrin was shown to be the main regulatory component of the total acid secretion and directly activated the parietal cell rather than by mediating gastric acid secretion through the release of histamine from the enterochromaffin-like cell, although both pathways were active. Under stimulated conditions with ACh, the gastrin and histamine components of the total acid secretion were not altered compared with levels observed under basal conditions, suggestive that ACh had no direct effect on the enterochromaffin-like cell and G cell. These data identify a new unique approach to investigate the regulation pathways active during acid secretion and the degree that they are utilized to drive total gastric acid secretion. The findings of this study will enhance our understanding on how these signaling mechanisms vary under pathophysiology or therapeutic management.

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Section: Discussioncontrasting

confidence: 50%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Studies Utilizing Isolated Ecl Cells and In Vitro Assays Havementioning

confidence: 99%

See 2 more Smart Citations

Simultaneous detection of gastric acid and histamine release to unravel the regulation of acid secretion from the guinea pig stomach

Bitziou

Patel

2012

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…The main functions of ghrelin are stimulation of growth hormone secretion and regulation of energy balance. In addition, loss of weight caused by lowmedium caloric diet, exercise, anorexia nervosa, organ (heart, lung, kidney and liver) failure, or malignant tumor cachexia could lead to increased level of ghrelin [10,11]. It has been indicated that in addition to being a short-term signal for eating regulation, ghrelin is also a long-term signal for nutritional status.…”

Section: Introductionmentioning

confidence: 99%

Study on the influence of acupoints selection on ghrelin and GHSR expression in gastric fundus tissue of DGP rats

Yue¹,

Li²,

Liu³

et al. 2018

biomedicalresearch

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Objective: To explore the acupoint selection compatibility according to different departments and to study the influence on ghrelin and GHSR expression in the gastric fundus in rats with Diabetic Gastroparesis (DGP). Methods: Sixty male SPF SD rats were given adaptive feeding for one week and were randomly divided into a blank control group, a model group, the Zusanli and Zhongwan (ZZ) group, the Zusanli and Neiguan group (ZN), as well as the Zusanli and non-meridian acupoint (ZNA) group, with 12 rats in each group. Except for the blank group, the other 48 rats were used to establish a DGP model by using streptozotocin. Acupuncture therapy was performed from the end of the tenth week for an additional four weeks. Then the rats were sacrificed and the serum and gastric fundus tissues were collected. ELISA assay was used to detect the ghrelin content in the serum. The expression of ghrelin positive cells in the gastric fundus tissue was detected by immunohistochemistry. The expression of ghrelin and GHSR mRNA in the gastric fundus tissue was detected by real-time PCR. Results: Compared with the blank group, the food intake and the expression of ghrelin and GHSR mRNA in the gastric fundus tissue in the model group were significantly increased, while the small intestinal propulsion rate, the ghrelin content in the serum and the gray value of the gastric fundus were significantly decreased (P<0.05). Compared with the model group, the expression of ghrelin and GHSR mRNA in the gastric fundus tissue, the small intestinal propulsion rate, the ghrelin content in the serum and the gray value of the gastric fundus were significantly increased (P<0.05). Compared with the ZZ group, the food intake were significantly increased in the ZN and the ZNA groups (P<0.05), and the expression of ghrelin and GHSR mRNA in the gastric fundus tissue of rats were significantly increased (P<0.05) but the ghrelin content in the serum in ZN group was significantly decreased (P<0.05). Conclusions: The improvement of gastrointestinal motility in DGP by acupuncture therapy was associated with the ghrelin content in the serum and GHSR expression in the gastric fundus. The treatment effect of local acupoints compatibility was better than that of the distal acupoints compatibility, and the acupoint selection according to department was one of the main influencing factors of acupoints compatibility.

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Measurement of Gastric Acid Secretion in the Anaesthetized Rat

Adami

Coruzzi

2010

CP Toxicology

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The protocols described in this unit are designed to assess the effects of substances on gastric acid secretion by the rat stomach, with the animal under general anesthesia. Both stimulatory and inhibitory effects of compounds can be evaluated and specific mechanisms of action can also be investigated. Acid secretion is induced by substances that directly activate parietal cell receptors (histamine and bethanechol), by indirect stimuli, like 2-deoxy-D-glucose, by electrical stimulation of vagal nerves, or by the peptide pentagastrin. Reference antisecretory drugs are represented by histamine H(2) receptor antagonists and proton pump inhibitors. This model allows the evaluation of complete dose-response curves together with a time-course of the secretory/antisecretory effects. Indirect effects involving activation or inhibition of vagal pathways are evaluated in intact animals by means of electrical vagal stimulation or in vagotomized animals.

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Gastric exocrine and endocrine secretion

Cited by 64 publications

References 53 publications

Simultaneous detection of gastric acid and histamine release to unravel the regulation of acid secretion from the guinea pig stomach

Simultaneous detection of gastric acid and histamine release to unravel the regulation of acid secretion from the guinea pig stomach

Study on the influence of acupoints selection on ghrelin and GHSR expression in gastric fundus tissue of DGP rats

Measurement of Gastric Acid Secretion in the Anaesthetized Rat

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