Gastric Juice Levels of Lactoferrin and<i>Helicobacter pylori</i>Infection

Nakao, Koji; Imoto, Ichiro; Gabazza, Esteban C.; Yamauchi, Koji; Yamazaki, Natsuko; Taguchi, Yukiko; Shibata, Toshihiro; Takaji, Satoshi; Ikemura, Norihisa; Misaki, Moriharu

doi:10.3109/00365529709025094

Cited by 33 publications

(19 citation statements)

References 26 publications

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“…23 A 70-kDa LF-binding protein highly specific for human LF was identified in outer membrane proteins of H. pylori grown under iron-limiting conditions, and this might constitute the iron uptake system of H. pylori. 24 Because the human LF concentration is estimated to be less than 0.05 mg/ml in gastric juice, 25,26 it is plausible to assume that a high dosage of orally administrated bLF might sequester iron from human LF in the stomach, resulting in inhibition of the growth of H. pylori. Also, recombinant human LF exerted an antibacterial action against 8 of 13 clinical isolates of H. pylori tested in vitro, but 5 of 13 strains were resistant to a high concentration (3 mg/ml) of recombinant human LF.…”

Section: Discussionmentioning

confidence: 99%

Bovine lactoferrin is effective to suppress Helicobacter pylori colonization in the human stomach: A randomized, double-blind, placebo-controlled study

Okuda

Nakazawa

Yamauchi

et al. 2005

Journal of Infection and Chemotherapy

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Section: Discussionmentioning

confidence: 99%

Bovine lactoferrin is effective to suppress Helicobacter pylori colonization in the human stomach: A randomized, double-blind, placebo-controlled study

Okuda

Nakazawa

Yamauchi

et al. 2005

Journal of Infection and Chemotherapy

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“…Therefore, the acquisition of iron plays a critical role in the host interaction with pathogenic bacteria, which have in consequence developed speci®c strategies for iron uptake. In the gastric mucosa, the amount of free ferric iron should be restricted due to the presence of secreted lactoferrin which removes iron by sequestration [26,27]. The presence of speci®c uptake systems for acquisition of iron from host-borne and nutritional sources, including lactoferrin [15,21], transferrin [40], citrate [21,40], and hemin [21,40,45,46] in H. pylori indicates that the iron acquisition of the pathogen is highly adapted to this unusual environment.…”

Section: Introductionmentioning

confidence: 99%

The ferric uptake regulator (Fur) homologue of Helicobacter pylori : functional analysis of the coding gene and controlled production of the recombinant protein in Escherichia coli

Bereswill¹,

Lichte²,

Greiner³

et al. 1999

Medical Microbiology and Immunology

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A homologue of the ferric uptake regulator protein Fur has recently been identified within the Helicobacter pylori genome. The promoterless gene on a plasmid did partially complement a fur-negative mutant of Escherichia coli, and was strongly positive in the Fur titration assay (FURTA). The genetic and functional characterization of the complete fur homologue performed in this study revealed that the gene is conserved among H. pylori strains ( > 95% identity), and does not carry nucleotide transitions in iron-resistant mutants of H. pylori. The fur homologue on a plasmid mediated full iron-dependent ferric uptake regulator activity in the fur-deficient mutant strains H1681 and H1780 of E. coli. Immunoblot analysis revealed that Fur from H. pylori cross-reacts with antibodies raised against Fur from E. coli. The fact that inactivation of the fur gene abolished the FURTA-positive phenotype in the E. coli indicator strain H1717, indicated that this phenotype is rather caused by the encoded protein than by real Fur titration. Subcloning of the fur gene into an expression vector allowed controlled production in E. coli, and purification of a recombinant version of the H. pylori Fur protein. In summary, the results confirm the function of the H. pylori Fur homologue as iron-dependent transcriptional repressor by its ability to interact with the Fur-regulated promoters of the genes fiu and fhuF in E. coli.

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“…Sequestration of iron in lactoferrin in the gastric mucosa is also suggested as possible. 15,16 In our study, 76 of 102 (74.50%) patients with IDA were H. pylori positive. Majority of the cases (53.32%) were in the age group of 20 -39 years.…”

Section: Hb (Gm%)mentioning

confidence: 51%

Role of Anti-Helicobacter Pylori Therapy in Iron Deficiency Anaemia- A Prospective Study

Parambil¹,

Shahulhameed²

2017

jemds

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BACKGROUNDAccumulating evidence suggests that Helicobacter pylori (H. Pylori) infection account for many unexplained cases of Iron Deficiency Anaemia (IDA), which may benefit from testing and treatment for H. pylori infection. This study attempts to evaluate the role of anti H. pylori treatment in management of iron deficiency anaemia among patients treated at a tertiary care centre in North India. MATERIALS AND METHODSThis was a prospective study which included 60 patients with IDA, proven positive for H. pylori by Rapid urease test and histopathological staining. They were divided into three groups (n= 20) and received different treatments, i.e. only anti-H. pylori therapy/ oral iron therapy plus anti-H. pylori therapy/ only oral iron therapy. Haemoglobin, RBC indices, Reti count, S. iron and S. ferritin were compared at the end of treatment with One-Way ANOVA.

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Gastric Juice Levels of Lactoferrin andHelicobacter pyloriInfection

Cited by 33 publications

References 26 publications

Bovine lactoferrin is effective to suppress Helicobacter pylori colonization in the human stomach: A randomized, double-blind, placebo-controlled study

Bovine lactoferrin is effective to suppress Helicobacter pylori colonization in the human stomach: A randomized, double-blind, placebo-controlled study

The ferric uptake regulator (Fur) homologue of Helicobacter pylori : functional analysis of the coding gene and controlled production of the recombinant protein in Escherichia coli

Role of Anti-Helicobacter Pylori Therapy in Iron Deficiency Anaemia- A Prospective Study

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